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Building a cell from its component parts /
This new volume of Methods in Cell Biology looks at building a cell from its component parts. Chapters cover such topics as engineering motor scaffolds, artificial cytoskeletons, interconnected droplet networks and artificial cells; building cytoskeletal systems and artificial actin cortex on pillar...
| Clasificación: | Libro Electrónico |
|---|---|
| Otros Autores: | , |
| Formato: | Electrónico eBook |
| Idioma: | Inglés |
| Publicado: |
Amsterdam :
Academic Press,
2015.
|
| Edición: | First edition. |
| Colección: | Methods in cell biology ;
v. 128. |
| Temas: | |
| Acceso en línea: | Texto completo Texto completo |
MARC
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| 245 | 0 | 0 | |a Building a cell from its component parts / |c edited by Jennifer Ross, Wallace F. Marshall. |
| 250 | |a First edition. | ||
| 264 | 1 | |a Amsterdam : |b Academic Press, |c 2015. | |
| 300 | |a 1 online resource : |b color illustrations | ||
| 336 | |a text |b txt |2 rdacontent | ||
| 337 | |a computer |b c |2 rdamedia | ||
| 338 | |a online resource |b cr |2 rdacarrier | ||
| 490 | 1 | |a Methods in cell biology, |x 0091-679X ; |v volume 128 | |
| 588 | 0 | |a Online resource; title from PDF title page (EBSCO, viewed May 28, 2015). | |
| 504 | |a Includes bibliographical references and index. | ||
| 505 | 0 | |a Front Cover; Methods in Cell Biology; Series Editors; Methods in Cell Biology; Copyright; Contents; Contributors; Preface; 1. In vitro systems for the study of microtubule-based cell polarity in fission yeast; Introduction; 1. Rationale; 2. Materials; 2.1 Materials for Method 1: Elongated Glass Wells with TiO2 Overhang; 2.1.1 Microfabrication; 2.1.2 Surface functionalization; 2.1.3 Assay; 2.2 Materials for Method 2: Elongated Water in Oil Emulsion Droplets; 2.2.1 Microfluidic chip; 3. Methods; 3.1 Method 1: Elongated Glass Wells with TiO2 Overhang; 3.1.1 Design. | |
| 505 | 8 | |a 3.1.2 Deposition of chromium and titanium oxide by electron-beam evaporation3.1.3 Postannealing of titanium oxide; 3.1.4 UV-lithography with S1813; 3.1.5 Reactive ion etching; 3.1.6 Wet etching with KOH; 3.1.7 Surface functionalization; 3.1.8 Assays; 3.2 Method 2: Elongated Water in Oil Emulsion Droplets; 3.2.1 Microfluidic design; 3.2.2 Masks for microfluidic chip fabrication; 3.2.3 SU-8 negative mould; 3.2.4 PDMS chip (Figure 5); 3.2.5 Microfluidic setup and droplet formation; 3.2.6 Lipids composition; 3.2.7 Assays; Discussion and Perspectives; Acknowledgments; References. | |
| 505 | 8 | |a 2. Microtubules, MAPs, and motor patternsIntroduction; 1. Methods; 2. Simple Filament-gliding Assay with Kinesin-1 and Microtubules; 2.1 Flow Chambers; 2.1.1 Materials; 2.1.2 Chamber construction; 2.1.3 Notes; 2.2 Gliding Assay Reagents and Buffers; 2.3 Experimental Details; 2.4 Analysis and Notes; 2.4.1 Kymographs; 2.4.2 Filament end tracking; 2.4.3 Notes; 3. Gliding Assay with MAP65 Cross-linkers to Visualize Dynamic Cross-linking; 3.1 MAP65 Purification; 3.1.1 Preparation reagents and buffers; 3.1.2 Protein purification; 3.1.2.1 Day 1; 3.1.2.2 Day 2; 3.1.2.3 Day 3. | |
| 505 | 8 | |a 3.2 Gliding Assay Buffers3.3 Experimental Details; 3.4 Analysis and Notes; 4. Cell-like Patterns from Gliding Prebundled Microtubule Filaments; 4.1 Reagents and Buffers; 4.2 Prebundling Microtubules; 4.3 Experimental Details; Discussion and Summary; Acknowledgments; References; 3. Self-organization of motors and microtubules in lipid-monolayered droplets; Introduction; 1. Materials; 1.1 Equipment; 1.1.1 Glass surface treatment and Polydimethylsiloxane (PDMS) chamber preparation; 1.1.2 Lipid-oil mixture preparation; 1.1.3 Buffer droplet in oil production; 1.1.4 Confocal fluorescence microscopy. | |
| 505 | 8 | |a 1.2 Reagents1.2.1 For glass surface treatment and PDMS chamber preparation; 1.2.2 For lipid-oil mixture preparation and buffer droplet in oil production; 1.3 Buffers and Solutions; 1.4 Proteins Used in the Assays; 2. Methods; 2.1 Glass Surface Treatment and PDMS Chamber Preparation; 2.1.1 Cleaning and hydrophobic surface treatment; 2.1.2 PDMS chambers; 2.2 Lipid-Oil Mixture Preparation; 2.3 Buffer Droplet in Oil Production; 2.3.1 Droplet solution preparation; 2.3.2 Resuspension of droplets in oil; 2.4 Confocal Fluorescence Microscopy; Results and Discussion; Acknowledgments; References. | |
| 520 | |a This new volume of Methods in Cell Biology looks at building a cell from its component parts. Chapters cover such topics as engineering motor scaffolds, artificial cytoskeletons, interconnected droplet networks and artificial cells; building cytoskeletal systems and artificial actin cortex on pillar arrays; reconstituting membrane fission, actin bundles at membranes, actin cortex in droplets, dynein-dynactin mediated cargo transport, MinD systems, protein-lipid machines that transform membranes, protein interactions and signaling on artificial lipid bilayers. With cutting-edge material, this c. | ||
| 650 | 0 | |a Cell culture. | |
| 650 | 1 | 2 | |a Cytological Techniques. |
| 650 | 6 | |a Cellules |x Culture. |0 (CaQQLa)201-0005038 | |
| 650 | 7 | |a SCIENCE |x Life Sciences |x Anatomy & Physiology. |2 bisacsh | |
| 650 | 7 | |a Cell culture. |2 fast |0 (OCoLC)fst00850172 | |
| 655 | 4 | |a Internet Resources. | |
| 700 | 1 | |a Ross, Jennifer, |e editor. | |
| 700 | 1 | |a Marshall, Wallace F., |d 1968- |e editor. | |
| 773 | 0 | |t Methods in cell biology. | |
| 773 | 0 | |t Elsevier. | |
| 776 | 0 | 8 | |i Print version: |a Ross, Jennifer. |t Building a Cell from Its Component Parts. |d Burlington : Elsevier Science, �2015 |z 9780128024508 |
| 830 | 0 | |a Methods in cell biology ; |v v. 128. | |
| 856 | 4 | 0 | |u https://sciencedirect.uam.elogim.com/science/book/9780128024508 |z Texto completo |
| 856 | 4 | 0 | |u https://sciencedirect.uam.elogim.com/science/bookseries/0091679X/128 |z Texto completo |