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Membrane proteins : Production and functional characterization /
Membrane Proteins - Production and Function Characterization a volume of Methods in Enzymology, encompasses chapters from the leading experts in the area of membrane protein biology. The chapters provide a brief overview of the topics covered and also outline step-by-step protocol. Illustrations and...
| Clasificación: | Libro Electrónico |
|---|---|
| Otros Autores: | |
| Formato: | Electrónico eBook |
| Idioma: | Inglés |
| Publicado: |
Amsterdam, Netherlands ; Boston, Mass. :
Elsevier/ Academic Press,
2015.
|
| Colección: | Methods in enzymology ;
v. 556. |
| Temas: | |
| Acceso en línea: | Texto completo |
MARC
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| 082 | 0 | 4 | |a 572/.696 |2 23 |
| 245 | 0 | 0 | |a Membrane proteins : |b Production and functional characterization / |c edited by Arun K. Shukla. |
| 246 | 3 | |a Production and functional characterization | |
| 264 | 1 | |a Amsterdam, Netherlands ; |a Boston, Mass. : |b Elsevier/ Academic Press, |c 2015. | |
| 264 | 4 | |c �2015 | |
| 300 | |a 1 online resource (xxiv, 651 pages and 14 pages of plates) : |b illustrations (some color) | ||
| 336 | |a text |b txt |2 rdacontent | ||
| 337 | |a computer |b c |2 rdamedia | ||
| 338 | |a online resource |b cr |2 rdacarrier | ||
| 347 | |a text file |2 rda | ||
| 490 | 1 | |a Methods in enzymology ; |v volume 556 | |
| 505 | 0 | |a Engineering Escherichia coli for functional expression of membrane proteins -- ACEMBLing a multiprotein transmembrane complex: the functional SecYEG-SecDF-YajC-YidC holotranslocon protein secretase/insertase -- Expression of purification of OsVDAC4 -- Membrane protein expression in Lactococcus lactis -- An unconventional anaerobic membrane protein production system based on Wolinella succinogenes -- Membrane protein expression and analysis in yeast -- Heterologous expression of G-protein-coupled receptors in yeast -- Recombinant G protein-coupled receptor expression in Saccharomyces cerevisiae for protein characterization -- Baculovirus-mediated expression of GPCRs in insect cells -- Expression of membrane proteins in the eyes of transgenic Drosophila melangaster -- Rapid method to express and purify human membrane protein using the Xenopus Oocyte system for functional and low-resolution structural anlaysis -- Expression of G protein-coupled receptors in mammalian cells -- Construction of stable mammalian cell lines for inducible expression of G protein-coupled receptors -- Rapid and facile recombinant expression of bovine rhodopsin in HEK293S GnTI- cells using a PiggyBac inducible system -- Semliki forest virus-based expression of recombinant GPCRs. | |
| 505 | 0 | |a Screening for lipid requirements of membrane proteins by combining cell-free expression with nanodiscs -- Liposome reconstitution and transport assay for recombinant transporters -- Lipid reconstitution and recording of recombinant ion channels -- Reconstitution of membrane proteins: a GPCR as an example -- Ion channel reporter for monitoring the activity of engineered GPCRs -- Methods for labeling skeletal muscle ion channels site-specifically with fluorophores suitable for FRET-based structural analysis -- Structural and fucntional studies of NirC from Salmonella typhimurium -- Surface plasmon resonance analysis of seven-transmembrane receptors -- Cross-linking strategies to study peptide ligand-receptor ineractions -- From recombinant expression to crystals: a step-by-step guide to GPCR crystallography -- Structure-based biophysical analysis of the interaction of rhodopsin with G protein and arrestin. | |
| 520 | |a Membrane Proteins - Production and Function Characterization a volume of Methods in Enzymology, encompasses chapters from the leading experts in the area of membrane protein biology. The chapters provide a brief overview of the topics covered and also outline step-by-step protocol. Illustrations and case example images are included wherever appropriate to help the readers understand the schematics and general experimental outlines. | ||
| 504 | |a Includes bibliographical references and indexes. | ||
| 546 | |a English. | ||
| 588 | |a Description based on online resource; title from digital title page (viewed on February 26, 2021). | ||
| 650 | 0 | |a Membrane proteins. | |
| 650 | 0 | |a Recombinant proteins. | |
| 650 | 1 | 2 | |a Membrane Proteins |0 (DNLM)D008565 |
| 650 | 2 | |a Recombinant Proteins |0 (DNLM)D011994 | |
| 650 | 6 | |a Prot�eines membranaires. |0 (CaQQLa)201-0033976 | |
| 650 | 6 | |a Prot�eines recombinantes. |0 (CaQQLa)201-0217641 | |
| 650 | 7 | |a SCIENCE |x Life Sciences |x Biochemistry. |2 bisacsh | |
| 650 | 7 | |a Membrane proteins |2 fast |0 (OCoLC)fst01015850 | |
| 650 | 7 | |a Recombinant proteins |2 fast |0 (OCoLC)fst01091505 | |
| 655 | 4 | |a Internet Resources. | |
| 655 | 4 | |a Plates. | |
| 700 | 1 | |a Shukla, Arun Kumar, |e editor. | |
| 776 | 0 | 8 | |i Print version: |t Membrane proteins. |d Amsterdam, Netherlands ; Boston, Mass. : Elsevier/ Academic Press, 2015 |z 9780128015216 |w (OCoLC)900032183 |
| 830 | 0 | |a Methods in enzymology ; |v v. 556. |x 0076-6879 | |
| 856 | 4 | 0 | |u https://sciencedirect.uam.elogim.com/science/bookseries/00766879/556 |z Texto completo |