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Conceptual background and bioenergetic/mitochondrial aspects of oncometabolism. Volume 542 /
Volume 542 of Methods in Enzymology continues the legacy of this premier serial with quality chapters authored by leaders in the field. This new volume covers research methods providing a theoretical overview on metabolic alterations of cancer cells and a series of protocols that can be employed to...
| Clasificación: | Libro Electrónico |
|---|---|
| Otros Autores: | , |
| Formato: | Electrónico eBook |
| Idioma: | Inglés |
| Publicado: |
Amsterdam ; Boston :
Elsevier/Academic Press,
2014.
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| Edición: | First edition. |
| Colección: | Methods in enzymology ;
volume 542. |
| Temas: | |
| Acceso en línea: | Texto completo Texto completo |
Tabla de Contenidos:
- Metabolic alterations of cancer cells / Marco Sciacovelli, Edoardo Gaude, Mika Hilvo, and Christian Frezza
- Autophagy and cancer metabolism / Juliet Goldsmith, Beth Levine, and Jayanta Debnath
- Regulation of cancer metabolism by oncogenes and tumor suppressors / Raffaella Iurlaro, Clara Luc�ia Le�on-Annicchiarico, and Cristina Mu�noz-Pinedo
- Cross talk between cell death regulation and metabolism / Simone Fulda
- Techniques to monitor glycolysis / Tara TeSlaa and Michael A. Teitell
- Measurement of enolase activity in cell lysates / Keigo Fukano and Kazuhiro Kimura / Extracellular flux analysis to monitor glycolytic rates and mitochondrial oxygen consumption / Martin Pelletier, Leah K. Billingham, Madhu Ramaswamy, and Richard M. Siegel
- Conventional techniques to monitor mitochondrial oxygen consumption / H�el�ene Simonnet, Arnaud Vigneron, and Jacques Pouyss�egur
- Use of safranin for the assessment of mitochondrial membrane potential by high-resolution respirometry and fluorometry / Gerhard Krumschnabel, Andrea Eigentler, Mario Fasching, and Erich Gnaiger
- Kinetic analysis of local oxygenation and respiratory responses of mammalian cells using intracellular oxygen-sensitive probes and time-resolved fluorometry / Alexander V. Zhdanov, Ruslan I. Dmietriev, James Hynes, and Dmitri B. Papkovsky
- Cell-based measurements of mitochondrial function in human subjects / Ju-Gyeong Kang, Ping-yuan Wang, and Paul M. Hwang
- Use of chemical probes to detect mitochondrial ROS by flow cytometry and spectrofluorometry / Jing Chen and Clayton E. Mathews
- Methods to monitor ROS production by fluorescence microscopy and fluorometry / Aleksandra Wojtala, Massimo Bonora, Dominika Malinska, Paolo Pinton, Jerzy Duszynski, and Mariusz R. Wieckowski
- Genetically encoded redox sensor / Wai Kan Chiu, Atif Towheed, and Michael J. Palladino
- Use of genetically encoded sensors to monitor cytosolic ATP/ADP ratio in living cells / Andrei I. Tarasov and Guy A. Rutter
- Methods to monitor and compare mitochondrial and glycolytic ATP production / Simone Patergnani, Federica Baldassari, Elena De Marchi, Agnieszka Karkucinska-Wieckowska, Mariusz R. Wieckowski, and Paolo Pinton
- Measurement of ADP-ATP exchange in relation to mitochondrial transmembrane potential and oxygen consumption / Christos Chinopoulos, Gergely Kiss, Hibiki Kawamata, and Anatoly A. Starkov
- Real-time assessment of the metabolic profile of living cells with genetically encoded NADH sensors / Yuzheng Zhao, Yi Yang, and Joseph Loscalzo
- 13C isotope-assisted methods for quantifying glutamine metabolism in cancer cells / Jie Zhang, Woo Suk Ahn, Paulo A. Gameiro, Mark A. Keibler, Zhe Zhang, and Gregory Stephanopoulos
- Measurement of fatty acid oxidation rates in animal tissues and cell lines / Frank K. Huynh, Michelle F. Green, Timothy R. Koves, and Matthew D. Hirschey
- Methods to assess lipid accumulation in cancer cells / J�rgen Sikkeland, Yang Jin, and Fahri Saatcioglu
- Analysis of hypoxia-induced metabolic preprogramming / Chendong Yang, Lei Jiang, Huafeng Zhang, Larissa A. Shimoda, Ralph J. Bernardinis, and Gregg L. Semenza.