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Mitochondrial function. Volume 547 /
| Clasificación: | Libro Electrónico |
|---|---|
| Otros Autores: | , |
| Formato: | Electrónico eBook |
| Idioma: | Inglés |
| Publicado: |
Amsterdam :
Academic Press,
2014.
|
| Colección: | Methods in enzymology ;
v. 547. |
| Temas: | |
| Acceso en línea: | Texto completo Texto completo |
MARC
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| 245 | 0 | 0 | |a Mitochondrial function. |n Volume 547 / |c edited by Anne N. Murphy, David C. Chan. |
| 264 | 1 | |a Amsterdam : |b Academic Press, |c 2014. | |
| 264 | 4 | |c �2014 | |
| 300 | |a 1 online resource (xx, 486 pages, 22 unnumbered pages of plates) : |b illustrations (chiefly color). | ||
| 336 | |a text |b txt |2 rdacontent | ||
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| 490 | 1 | |a Methods in enzymology, |x 0076-6879 ; |v volume 547 | |
| 500 | |a Title from PDF title page (ScienceDirect, viewed November 29, 2014). | ||
| 505 | 0 | |a 1.High-Content Functional Genomic Screening to Identify Novel Regulators of the PINK1 -- Parkin Pathway / Robert A. Screaton -- 1.Introduction and Theory -- 2.General Screen Design Strategy -- 3.RNAi Screen for Genes Involved Required for PARK2 Translocation -- 4.Equipment -- 5.Materials -- 6.Genome Screen Protocol -- Acknowledgments -- References -- 2.Measurement of Mitochondrial Turnover and Life Cycle Using MitoTimer / Orian S. Shirihai -- 1.Introduction -- 2.Expressing MitoTimer in Cells -- 3.Imaging MitoTimer -- 4.Interpreting MitoTimer Readout -- 5.Summary -- References -- 3.Monitoring Mitophagy in Mammalian Cells / Quan Chen -- 1.Introduction -- 2.Transmission Electron Microscopy -- 3.Western Blot Analysis of Mitophagy -- 4.Fluorescence Methods for Analyzing Mitophagy -- 5.Analyzing Mitophagy Using Mitochondria Mass -- 6.Mitochondria-Targeting Probes for Mitophagy Assay -- 7.Mitophagy Inducer and Inhibitors -- 8.Future Perspectives -- Acknowledgments -- References -- 4.Photoactivatable Green Fluorescent Protein-Based Visualization and Quantification of Mitochondrial Fusion and Mitochondrial Network Complexity in Living Cells / Brian A. Roelofs -- 1.Introduction -- 2.Visualization and Quantification of Mitochondrial Fusion in Living Cells -- 3.Estimation of Relative Size of Mitochondrial Units and Mitochondrial Network Complexity -- 4.Time-Lapse Imaging of Mitochondria in Living Cells: General Considerations -- 5.Concluding Remarks: Other Potential Applications of Mito-PAGFP -- Acknowledgments -- References -- 5.Characterization of Mitochondrial Transport in Neurons / Zu-Hang Sheng -- 1.Introduction -- 2.Analyses of Mitochondrial Transport in Embryonic Neuron Cultures -- 3.Analyses of Mitochondrial Transport in Adult Neuron Cultures -- 4.Characterization of Mitochondrial Transport at Synapses -- 5.Summary -- Acknowledgments -- References -- 6.Imaging of Mitochondrial Dynamics in Motor and Sensory Axons of Living Mice / Jordi Magrane -- 1.Introduction -- 2.Protocols to Image Mitochondrial Transport in the Peripheral Nerves of Living Mice -- 3.Imaging of Mitochondrial Dynamics in the Peripheral Nerves of Living Mice -- 4.Conclusions -- Acknowledgments -- References -- 7.Analyzing Mitochondrial Dynamics in Mouse Organotypic Slice Cultures / David C. Chan -- 1.Parasagittal Slice Cultures of the Basal Ganglia -- 2.Cerebellar Slice Cultures -- 3.Monitoring Mitochondrial Dynamics in Slice Cultures -- References -- 8.Analysis of Mitochondrial Traffic in Drosophila / Peter J. Hollenbeck -- 1.Introduction -- 2.Dissection Methods for Drosophila Larvae -- 3.Imaging -- 4.Analysis -- 5.Conclusions -- Acknowledgment -- References -- 9.In Vivo Imaging of Mitochondria in Intact Zebrafish Larvae / Thomas Misgeld -- 1.Introduction -- 2.Preparation of Transgenic MitoFish for Live Imaging -- 3.Visualizing Axonal Transport of Mitochondria in Zebrafish Sensory Neurons -- 4.Processing and Quantification of Imaging Files -- 5.Conclusions -- Acknowledgments -- References -- 10.The Use of miniSOG in the Localization of Mitochondrial Proteins / Guy A. Perkins -- 1.Introduction -- 2.Requirements for CLEM Labeling -- 3.miniSOG Features -- 4.Resolution -- 5.Photooxidation Protocol for a Monolayer of Cultured Cells -- 6.Photooxidation Protocol for Tissues -- 7.Example of miniSOG Use with MCU -- 8.Conclusions and Future Work -- Acknowledgments -- References -- 11.Assessing the Function of Mitochondria-Associated ER Membranes / Estela Area-Gomez -- 1.Introduction -- 2.Isolation of MAM -- 3.Assaying MAM Activity -- References -- 12.Measurement of ROS Homeostasis in Isolated Mitochondria / A. Ambrus -- 1.Importance of Quantifying Mitochondrial ROS Homeostasis -- 2.Detection of Mitochondrial ROS Formation -- 3.Mitochondrial Elimination of H2O2 -- 4.Methods for the Detection of Oxidative Stress -- Acknowledgments -- References -- 13.Use of Potentiometric Fluorophores in the Measurement of Mitochondrial Reactive Oxygen Species / Brian A. Roelofs -- 1.Introduction -- 2.ROS Detection Using MitoSOX -- Subcellular Localization and Fluorescence Yield -- 3.Validating MitoSOX Using a Negative Control -- 4.Choosing a Correct MitoSOX Loading Paradigm -- Additional Considerations -- 5.Is MitoSOX Imaging Useful? -- 6.Preparation of Primary Rat Cortical Neurons for Imaging -- 7.Optimizing MitoSOX Concentration and Establishing Mitochondrial Localization -- 8.Imaging Using MitoSOX -- Acknowledgment -- References -- 14.Spatial, Temporal, and Quantitative Manipulation of Intracellular Hydrogen Peroxide in Cultured Cells / Rajiv R. Ratan -- 1.Introduction -- 2.Production of H2O2 Using DAAO -- 3.Two-Photon Fluorescence Imaging of H2O2 -- 4.Summary -- Acknowledgments -- References -- 15.Biochemical and Biophysical Methods for Studying Mitochondrial Iron Metabolism / Tracey A. Rouault -- 1.Introduction -- 2.Measurement of Total Iron Concentration -- 3.In Situ Analysis of Iron in the Mitochondria -- 4.Biophysical Methods for Studying Iron in Isolated Mitochondria -- 5.Conclusions -- Acknowledgments -- References -- 16.Analysis and Interpretation of Microplate-Based Oxygen Consumption and pH Data / Martin Jastroch -- 1.Introduction -- 2.The Oxygen Consumption Rate -- 3.The Extracellular Acidification Rate -- Summary -- Acknowledgments -- References -- 17.Imaging Changes in the Cytosolic ATP-to-ADP Ratio / Gary Yellen -- 1.Introduction -- 2.Methods -- 3.Expected Results -- 4.Summary -- References -- 18.The Use of Mitochondria-Targeted Endonucleases to Manipulate mtDNA / Carlos T. Moraes -- 1.Mitochondrial DNA -- 2.Mitochondria-Targeted Restriction Nucleases to Cleave mtDNA and Model OXPHOS Diseases -- 3.mtDNA Heteroplasmy and Approaches to Alter the Balance Between Wild-Type and Mutant mtDNA -- 4.mtDNA Heteroplasmy Shift Using Restriction Endonucleases -- 5.Designer Endonucleases for the Modulation of mtDNA Heteroplasmy -- 6.mtDNA Heteroplasmy Shift Using Zinc-Finger Nucleases -- 7.Heteroplasmy Shift Using TAL Effector Nucleases -- 8.Single-Strand Annealing Assay to Analyze the Efficacy and Specificity of Designer Nuclease -- 9.The Use of Cybrid Cells to Test Approaches to Change Mitochondrial DNA Heteroplasmy -- 10.Immunodetection and Mitochondrial Localization in Cells and Tissues -- 11.Changing mtDNA Heteroplasmy in Cultured Cells with mito-TALENs -- 12.Evaluation of the mtDNA Content -- 13.Future Perspectives -- Acknowledgments -- References -- 19.Induced Pluripotent Stem Cell-Derived Models for mtDNA Diseases / Riikka H. Hamalainen -- 1.Introduction -- 2.Induced Pluripotent Stem Cells -- 3.Mitochondrial Disease -- 4.Conclusion -- 5.Generation of iPSCs from mtDNA Disease Patients -- Acknowledgment -- References -- 20.The Use of 18F-BCPP-EF as a PET Probe for Complex I Activity in the Brain / Hideo Tsukada -- 1.Introduction -- 2.Design and Assessment of Candidate Compounds for MC-I Imaging Probes -- 3.Application for Stroke/Ischemic Damage Imaging -- 4.Application for Imaging of Aging Effects on MC-I Activity -- 5.Conclusion -- References -- 21.MR OEF Imaging in MELAS / Sheng Xie -- 1.Introduction of Magnetic Resonance Imaging Techniques -- 2.Significance of OEF Imaging in MELAS -- 3.Cerebral OEF Changes In Our Study of MELAS -- References. | |
| 650 | 0 | |a Mitochondrial pathology. | |
| 650 | 0 | |a Mitochondrial DNA. | |
| 650 | 0 | |a Neurology |x Research. | |
| 650 | 2 | |a DNA, Mitochondrial |0 (DNLM)D004272 | |
| 650 | 6 | |a Maladies mitochondriales. |0 (CaQQLa)201-0215121 | |
| 650 | 6 | |a ADN mitochondrial. |0 (CaQQLa)201-0015244 | |
| 650 | 6 | |a Neurologie |x Recherche. |0 (CaQQLa)201-0011407 | |
| 650 | 7 | |a SCIENCE |x Life Sciences |x Anatomy & Physiology. |2 bisacsh | |
| 650 | 7 | |a Mitochondrial DNA |2 fast |0 (OCoLC)fst01024018 | |
| 650 | 7 | |a Mitochondrial pathology |2 fast |0 (OCoLC)fst01024022 | |
| 650 | 7 | |a Neurology |x Research |2 fast |0 (OCoLC)fst01036400 | |
| 655 | 4 | |a Internet Resources. | |
| 700 | 1 | |a Murphy, Anne N. |q (Anne Neville), |e editor. | |
| 700 | 1 | |a Chan, David C., |d 1966- |e editor. | |
| 776 | 0 | 8 | |i Print version: |t Mitochondrial function. |b First edition. |d Waltham, MA : Elsevier Academic Press, 2014 |z 9780128014158 |w (OCoLC)900218702 |
| 830 | 0 | |a Methods in enzymology ; |v v. 547. |x 0076-6879 | |
| 856 | 4 | 0 | |u https://sciencedirect.uam.elogim.com/science/book/9780128014158 |z Texto completo |
| 856 | 4 | 0 | |u https://sciencedirect.uam.elogim.com/science/bookseries/00766879/547 |z Texto completo |
| 856 | 4 | 0 | |u https://sciencedirect.uam.elogim.com/science/bookseries/00766879/547 |z Texto completo |