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Cell separation. methods and selected applications / Volume 4 :

Detalles Bibliográficos
Clasificación:Libro Electrónico
Otros Autores: Pretlow, Thomas G. (Editor ), Pretlow, Theresa P. (Editor )
Formato: Electrónico eBook
Idioma:Inglés
Publicado: New York : Academic Press, 1987.
Temas:
Acceso en línea:Texto completo
Tabla de Contenidos:
  • Front Cover; Cell Separation: Methods and Selected Applications; Copyright Page; Table of Contents; Preface; Chapter 1. Separation and Characterization of Liver Cells; I. Introduction; II. Characterization of Liver Cells; III. Preparation of Isolated Liver Cells; IV. Conclusions; ACKNOWLEDGMENTS; REFERENCES; Chapter 2. Purification and Characterization of Epidermal Langerhans Cells; I. Introduction; II. Cellular Constituents of the Epidermis; III. Characterization of Epidermal Langerhans Cells; IV. Enrichment of Epidermal Langerhans Cells; ACKNOWLEDGMENT; REFERENCES
  • Chapter 3. Purification and Culture of Oval Cells from Rat LiverI. Biological Importance of Oval Cells: Does Normal Adult Liver Contain Stem Cells?; II. Isolation and Characterization of Oval Cells and Comparison with Other Liver Cell Types from Normal and Carcinogen-Treated Rats; III. Growth of Liver Epithelial Cells in Vitro; IV. Perspectives; ACKNOWLEDGMENTS; REFERENCES; Chapter 4. Isolation and Characterization of Lymphocytes from Mature Mouse Liver; I. Introduction; II. Preparation of Suspensions of Cells from Liver; III. Separation of Lymphocytes from Mouse Liver
  • IV. Further Characterization of Lymphocytes from LiverV. Concluding Remarks; ACKNOWLEDGMENTS; REFERENCES; Chapter 5. A Comparison of Cell Separations Obtained with Centrifugal Elutriation and Sedimentation at Unit Gravity; I. Introduction; II. A Theoretical Comparison and Factors Influencing Separation; III. Applications; IV. Summary and Conclusions; ACKNOWLEDGMENTS; REFERENCES; Chapter 6. Limiting Dilution Assays for the Separation, Characterization, and Quantitation of Biologically Active Particles and Their Clonal Progeny; I. Introduction; II. Sample LDAs: SHPM Validity Tests
  • III. Sample LDAs: Sample EstimatorsIV. Population LDAs; V. Assay Design; VI. Comparative Experiments; VII. Clonal Analysis; VIII. Partition Analysis; IX. Conclusion; ACKNOWLEDGMENTS; REFERENCES; Chapter 7. Purification and Functional Evaluation of Human Polymorphonuclear Leukocytes; I. Introduction; II. Background; III. Techniques for the Isolation of Polymorphonuclear Neutrophils; IV. Considerations in Choosing a Technique for the Purification of Polymorphonuclear Neutrophils; V. Functional Analysis of Circulating Human Polymorphonuclear Neutrophils Isolated by Different Methods
  • VI. Concluding RemarksACKNOWLEDGMENTS; REFERENCES; Chapter 8. Fabrication of a Manually Operable Countercurrent Distribution Apparatus and Its Application for Separation of Cells in Aqueous Two-Phase Polymer System; l. Introduction; II. Countercurrent Distribution (CCD); III. Separation of Rat Testicular Cells by Manually Operable CCD Apparatus; IV. Conclusion; ACKNOWLEDGMENTS; REFERENCES; Chapter 9. Separation and Characterization of Phagocytes from Human Colon; I. Introduction; II. Dissociation of Mucosal Cells; III. Enrichment of Mucosal Phagocytes