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Methods in virology /
| Clasificación: | Libro Electrónico |
|---|---|
| Otros Autores: | , |
| Formato: | Electrónico eBook |
| Idioma: | Inglés |
| Publicado: |
New York ; London :
Academic Press,
1984.
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| Temas: | |
| Acceso en línea: | Texto completo |
Tabla de Contenidos:
- Front Cover; Methods in Virology; Copyright Page; Table of Contents; Contributors; Preface; Contents of Previous Volumes; Chapter 1. Monoclonal Antibody Techniques Applied to Viruses; I. Introduction; II. Immunization Protocol; III. Preparation of Cells; IV. Hybridization; V. Maintenance and Specificity Testing of Hybridomas; VI. Cloning of Hybridomas; VII. Determination of Immunoglobulin Class; VIII. Preparation of Ascites Fluid; IX. Concentration and Purification of Immunoglobulins; X. Characterization of Viral Antigens Using Monoclonal Antibodies; XI. Prospectus.
- XII. Appendix: Preparation of ReagentsREFERENCES; Chapter 2. Competition Radioimmunoassays forCharacterization of Antibody Reactionsto Viral Antigens; I. Introduction; II. Preparation and Characterization of Reagents; III. Basic Methodologies; IV. Applicability to Identification of Antibody Populations; V. Applicability to Characterization of Viral Antigens; VI. Biological Tracking of Viral Proteins; VII. Concluding Comments; ACKNOWLEDGMENT; REFERENCES; Chapter 3. Enzyme Immunosorbent Assays in Plant Virology; I. Introduction; II. Principles of Enzyme Immunosorbent Assay.
- III. Preparation of SampleIV. Production of Antiserum; V. Choice of Enzyme and Preparation of Conjugate; VI. Substrates; VII. Choice of Solid Phase; VIII. Types of ELISA Procedure; IX. Evaluation of Reagents and Analysis of Results; X. Conclusions; XI. Appendix: Buffer Formulations; REFERENCES; Chapter 4. Electron Microscopy for the Identification of Plant Viruses inin Vitro Preparations; I. Introduction; II. The Electron Microscope; III. Support Films; IV. Calibration of Magnification; V. Negative Staining; VI. Metal Shadowing; VII. Immunoelectron Microscopy; VIII. Imaging of Nucleic Acids.
- ACKNOWLEDGMENTSREFERENCES; Chapter 5. Cloning and Expression of ViralAntigens in Escherichia coli and Other Microorganisms; I. Introduction; II. Expression of Foreign Coding Sequences in Escherichia coli; III. Hepatitis B Virus; IV. Foot-and-Mouth Disease Virus; V. Poliovirus: Purification of Polio RNA and Cloning of Polio cDNA; VI. Influenza Virus; VII. Rabies Virus; VIII. Expression of Viral Antigens in Bacillus subtilis; IX. Expression of Viral Antigens in Saccharomyces cerevisiae; X. Conclusion; ACKNOWLEDGMENTS; REFERENCES; Chapter 6. Spot Hybridization for Detection of Viroids and Viruses.
- I. IntroductionII. PSTV Diagnosis by Nucleic Acid Spot Hybridization; III. Application to Other Viroid Diseases; IV. Application to Viral Diseases; V. Supplementary Methodologies; REFERENCES; Chapter 7. Detection of Viral Nucleic Acids by in Situ Hybridization; I. Introduction; II. Materials and Methods; III. Applications; ACKNOWLEDGMENTS; REFERENCES; Chapter 8. Exploring the Gene Organization of Baculoviruses; I. Introduction; II. Establishing the Physical Map of a Baculovirus; III. Mapping Mutuations of Baculoviruses; IV. Correlating Proteins with the Physical Map.