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Immunological methods /
| Clasificación: | Libro Electrónico |
|---|---|
| Otros Autores: | , |
| Formato: | Electrónico eBook |
| Idioma: | Inglés |
| Publicado: |
Orlando :
Academic P.,
1985.
|
| Temas: | |
| Acceso en línea: | Texto completo |
Tabla de Contenidos:
- Front Cover; Immunological Methods; Copyright Page; Table of Contents; Contributors; Preface; Contents of Previous Volumes; Abbreviations List; Chapter 1. Methods in Molecular Immunology: Chromosomal Walking in the Major Histocompatibility Complex; I. INTRODUCTION; II. MATERIALS; III. CONSTRUCTION OF COSMID LIBRARIES; IV. SCREENING OF COSMID LIBRARIES; V. CHARACTERIZATION OF COSMID CLONES; VI. APPRAISAL; REFERENCES; Chapter 2. DNA-Mediated Tranformation of Lymphoid Cells; I. INTRODUCTION; II. DOMINANT SELECTABLE MARKERS; III. TECHNIQUES OF STABLE GENE TRANSFER.
- IV. OTHER TECHNIQUES OF STABLE GENE TRANSFERREFERENCES; Chapter 3. Cloning of cDNAs with Vectors Permitting Expression in Host Cells; I. INTRODUCTION; II. GENERAL STRATEGIES AND THEIR PROBLEMS; III. EXPERIMENTAL PROCEDURES; IV. SOME COMMENTS; REFERENCES; Chapter 4. Construction of Vectors for Immunoglobulin Reverse Genetics; I. INTRODUCTION; II. GENERAL STRATEGY; III. MATERIAL AND STOCK SOLUTIONS; IV. EXPERIMENTAL PROCEDURES; V. EXAMPLES OF CONSTRUCTIONS; REFERENCES; Chapter 5. Strategies of Oligonucleotide Synthesis; I. INTRODUCTION; II. STRATEGIES; III. EXPERIMENTAL SECTION.
- IV. REAGENTS AND SOLVENTS THAT MUST BE PURIFIED OR PREPAREDREFERENCES; Chapter 6. High-performance Liquid Chromatography of Biologically Active Proteins in the Nanogram (Picomole) Range; I. INTRODUCTION; II. MATERIALS AND METHODS; III. SEPARATION BY SIZE: GEL PERMEATION; IV. SEPARATION BY CHARGE: ION EXCHANGE AND HYDROXYAPATITE; V. SEPARATION BY HYDROPHOBICITY: REVERSE PHASE; VI. QUANTITATION AND LIMITS OF DETECTION; VII. PROTEIN PURIFICATION; REFERENCES; Chapter 7. The HPLC of Immunoglobulins; I. INTRODUCTION; II. HPLC: THEORY, PRACTICE, AND EQUIPMENT; III. IMMUNOGLOBULINS ON HPLC.
- IV. DISCUSSIONREFERENCES; Chapter 8. Preparation of Liposomes Carrying Lymphocyte Membrane Proteins; I. INTRODUCTION; II. ISOLATION OF MEMBRANE PROTEINS; III. PREPARATION OF LIPOSOMES; IV. APPLICATIONS; REFERENCES; Chapter 9. Detection of Glycolipid Antigens with Monoclonal Antibodies; I. INTRODUCTION; II. PRELIMINARY TESTS; III. LIPID EXTRACTION; IV. SOLID PHASE RIA; V. HAPTEN INHIBITION AND HAPTEN BINDING; VI. THIN-LAYER CHROMATOGRAPHY; VII. CRITICAL APPRAISAL; REFERENCES; Chapter 10. Mapping of Novel B Lymphocyte Differentiation Antigens; I. INTRODUCTION.
- II. DEFINITION OF B CELL DIFFERENTIATION ANTIGENS BY NEGATIVE SELECTION ASSAYIII. TWO-DIMENSIONAL GEL ANALYSIS OF B CELL DIFFERENTIATION ANTIGENS; IV. APPENDIX; SUGGESTED READING; REFERENCES; Chapter 11. Use of Large-Scale Two-Dimensional ISODALT Gel Electrophoresis System in Immunology; I. OBJECTIVE; II. MATERIALS; III. METHODS; IV. CRITICAL APPRAISAL; REFERENCES; Chapter 12. Tissue Typing Using Biosynthetically Labeled Monoclonal Antibodies; I. INTRODUCTION; II. BIOSYNTHETIC LABELING; III. PURIFICATION; IV. ACTIVITY AND STABILITY; V. BINDING ASSAY; VI. APPLICATIONS AND LIMITATIONS.