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00000cam a2200000 i 4500 |
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SCIDIR_ocn892069063 |
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OCoLC |
| 005 |
20231120111803.0 |
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m o d |
| 007 |
cr cnu---unuuu |
| 008 |
141003s1985 flu o 000 0 eng d |
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|a OPELS
|b eng
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|c OPELS
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|d OCLCF
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| 019 |
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|a 898422338
|a 907115642
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| 020 |
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|a 9781483269634
|q (electronic bk.)
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| 020 |
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|a 1483269639
|q (electronic bk.)
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| 020 |
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|z 0124427030
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| 020 |
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|z 9780124427037
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| 035 |
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|a (OCoLC)892069063
|z (OCoLC)898422338
|z (OCoLC)907115642
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4 |
|a QR183
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| 072 |
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|a NAT
|x 019000
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|a SCI
|x 070030
|2 bisacsh
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| 082 |
0 |
4 |
|a 599.02/9/028
|2 22
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| 245 |
0 |
0 |
|a Immunological methods /
|c edited by Ivan Lefkovits and Benvenuto Pernis. V.3.
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| 264 |
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1 |
|a Orlando :
|b Academic P.,
|c 1985.
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| 300 |
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|a 1 online resource
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| 336 |
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|a text
|b txt
|2 rdacontent
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| 337 |
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|a computer
|b c
|2 rdamedia
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| 338 |
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|a online resource
|b cr
|2 rdacarrier
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| 588 |
0 |
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|a Print version record.
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| 504 |
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|a References.
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0 |
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|a Front Cover; Immunological Methods; Copyright Page; Table of Contents; Contributors; Preface; Contents of Previous Volumes; Abbreviations List; Chapter 1. Methods in Molecular Immunology: Chromosomal Walking in the Major Histocompatibility Complex; I. INTRODUCTION; II. MATERIALS; III. CONSTRUCTION OF COSMID LIBRARIES; IV. SCREENING OF COSMID LIBRARIES; V. CHARACTERIZATION OF COSMID CLONES; VI. APPRAISAL; REFERENCES; Chapter 2. DNA-Mediated Tranformation of Lymphoid Cells; I. INTRODUCTION; II. DOMINANT SELECTABLE MARKERS; III. TECHNIQUES OF STABLE GENE TRANSFER.
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| 505 |
8 |
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|a IV. OTHER TECHNIQUES OF STABLE GENE TRANSFERREFERENCES; Chapter 3. Cloning of cDNAs with Vectors Permitting Expression in Host Cells; I. INTRODUCTION; II. GENERAL STRATEGIES AND THEIR PROBLEMS; III. EXPERIMENTAL PROCEDURES; IV. SOME COMMENTS; REFERENCES; Chapter 4. Construction of Vectors for Immunoglobulin Reverse Genetics; I. INTRODUCTION; II. GENERAL STRATEGY; III. MATERIAL AND STOCK SOLUTIONS; IV. EXPERIMENTAL PROCEDURES; V. EXAMPLES OF CONSTRUCTIONS; REFERENCES; Chapter 5. Strategies of Oligonucleotide Synthesis; I. INTRODUCTION; II. STRATEGIES; III. EXPERIMENTAL SECTION.
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|a IV. REAGENTS AND SOLVENTS THAT MUST BE PURIFIED OR PREPAREDREFERENCES; Chapter 6. High-performance Liquid Chromatography of Biologically Active Proteins in the Nanogram (Picomole) Range; I. INTRODUCTION; II. MATERIALS AND METHODS; III. SEPARATION BY SIZE: GEL PERMEATION; IV. SEPARATION BY CHARGE: ION EXCHANGE AND HYDROXYAPATITE; V. SEPARATION BY HYDROPHOBICITY: REVERSE PHASE; VI. QUANTITATION AND LIMITS OF DETECTION; VII. PROTEIN PURIFICATION; REFERENCES; Chapter 7. The HPLC of Immunoglobulins; I. INTRODUCTION; II. HPLC: THEORY, PRACTICE, AND EQUIPMENT; III. IMMUNOGLOBULINS ON HPLC.
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| 505 |
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|a IV. DISCUSSIONREFERENCES; Chapter 8. Preparation of Liposomes Carrying Lymphocyte Membrane Proteins; I. INTRODUCTION; II. ISOLATION OF MEMBRANE PROTEINS; III. PREPARATION OF LIPOSOMES; IV. APPLICATIONS; REFERENCES; Chapter 9. Detection of Glycolipid Antigens with Monoclonal Antibodies; I. INTRODUCTION; II. PRELIMINARY TESTS; III. LIPID EXTRACTION; IV. SOLID PHASE RIA; V. HAPTEN INHIBITION AND HAPTEN BINDING; VI. THIN-LAYER CHROMATOGRAPHY; VII. CRITICAL APPRAISAL; REFERENCES; Chapter 10. Mapping of Novel B Lymphocyte Differentiation Antigens; I. INTRODUCTION.
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| 505 |
8 |
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|a II. DEFINITION OF B CELL DIFFERENTIATION ANTIGENS BY NEGATIVE SELECTION ASSAYIII. TWO-DIMENSIONAL GEL ANALYSIS OF B CELL DIFFERENTIATION ANTIGENS; IV. APPENDIX; SUGGESTED READING; REFERENCES; Chapter 11. Use of Large-Scale Two-Dimensional ISODALT Gel Electrophoresis System in Immunology; I. OBJECTIVE; II. MATERIALS; III. METHODS; IV. CRITICAL APPRAISAL; REFERENCES; Chapter 12. Tissue Typing Using Biosynthetically Labeled Monoclonal Antibodies; I. INTRODUCTION; II. BIOSYNTHETIC LABELING; III. PURIFICATION; IV. ACTIVITY AND STABILITY; V. BINDING ASSAY; VI. APPLICATIONS AND LIMITATIONS.
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| 650 |
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0 |
|a Immunology
|v Laboratory manuals.
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| 650 |
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0 |
|a Experimental immunology.
|
| 650 |
|
6 |
|a Immunologie
|0 (CaQQLa)201-0066630
|v Manuels de laboratoire.
|0 (CaQQLa)201-0376994
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| 650 |
|
6 |
|a Immunologie exp�erimentale.
|0 (CaQQLa)201-0084124
|
| 650 |
|
7 |
|a NATURE
|x Animals
|x Mammals.
|2 bisacsh
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| 650 |
|
7 |
|a SCIENCE
|x Life Sciences
|x Zoology
|x Mammals.
|2 bisacsh
|
| 650 |
|
7 |
|a Experimental immunology
|2 fast
|0 (OCoLC)fst00918456
|
| 650 |
|
7 |
|a Immunology
|2 fast
|0 (OCoLC)fst00968006
|
| 655 |
|
7 |
|a Laboratory manuals
|2 fast
|0 (OCoLC)fst01920776
|
| 655 |
|
7 |
|a Laboratory manuals.
|2 lcgft
|
| 655 |
|
7 |
|a Manuels de laboratoire.
|2 rvmgf
|0 (CaQQLa)RVMGF-000001214
|
| 700 |
1 |
|
|a Lefkovits, Ivan.
|
| 700 |
1 |
|
|a Pernis, Benvenuto.
|
| 776 |
0 |
8 |
|i Print version:
|t Immunological methods
|z 0124427030
|w (OCoLC)847307771
|
| 856 |
4 |
0 |
|u https://sciencedirect.uam.elogim.com/science/book/9780124427037
|z Texto completo
|
