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Electrophysiology and microinjection /
| Clasificación: | Libro Electrónico |
|---|---|
| Otros Autores: | |
| Formato: | Electrónico eBook |
| Idioma: | Inglés |
| Publicado: |
San Diego, California :
Academic Press, Inc.,
[1991]
|
| Colección: | Methods in neurosciences ;
v. 4. |
| Temas: | |
| Acceso en línea: | Texto completo |
Tabla de Contenidos:
- Cover image; Title page; Table of Contents; Copyright page; Contributors to Volume 4; Preface; Methods in Neurosciences; Section I: Recording Methodologies; Chapter 1: Single-Unit Recording in Conscious Sheep; Publisher Summary; Introduction; Methods; Results; Quality and Stability of Recordings; Chapter [2]: Single-Unit Recording from Pontomedullary Neuraxis in Awake, Freely Behaving Animals; Publisher Summary; Introduction; Technical Descriptions; Results; Discussion; Acknowledgment; Chapter 3: Measurement of Whole-Cell Calcium Current in Cardiac Myocytes; Publisher Summary; Introduction.
- ProceduresConclusions; Chapter 4: Measurement of Noninactivating Calcium Current in Smooth Muscle Cells; Publisher Summary; Cell Isolation and Measurement of Membrane Current; Measurement of Noninactivating Ca2+ Current; Ca2+ Window Current and Noninactivating Ca2+ Current; Advantages and Limitations of the Method; Acknowledgment; Chapter 5: Two-Suction Electrode Voltage-Clamp Recording; Publisher Summary; Introduction; Voltage-Clamp Circuit; Procedures for Voltage Clamping; Conclusion; Acknowledgment; Section II: Voltage Clamping and Voltammetry.
- Chapter 6: Voltage-Clamp Measurement of Steady State Currents and Isotope FluxPublisher Summary; Introduction; Methods; Results; Discussion; Acknowledgment; Chapter 7: Current Pump-Assisted Voltage-Clamp Apparatus; Publisher Summary; Introduction; Theory; Preparation and Setup; How to Use Current Pump-Assisted Voltage Clamp; Discussion; Acknowledgments; Chapter 8: X-Ray Microprobe Analysis of Voltage-Clamped Single Heart Ventricular Myocytes; Publisher Summary; Introduction; Methods; EPMA Quantifies Calcium Loading via ICa2+
- EPMA Identification of Calcium Buffers and Calcium Stores at High Cellular Calcium LoadPaired Pulsing Increases Myoplasmic Diastolic Total [Ca] (EPMA); Total Myoplasmic [Ca] during Systole; Total [Ca] of Mitochondria Increases during Systole; Activity-Related Changes in Total [Ca] of Sarcoplasmic Reticulum; Acknowledgment; Chapter [9]: In Vivo Voltammetry; Publisher Summary; Introduction; Principles; Objectives; Electrodes; Voltammetry; Apparatus; Current Applications; Summary; Acknowledgment.
- Chapter [10]: Simultaneous in Vivo Voltammetric and Electrophysiological Recording with Carbon Fiber MicroelectrodesPublisher Summary; Introduction; Extracellular Unit Recording; Manufacture of Carbon Fiber Electrodes; Time-Share Technique of Single-Unit and Fast Cyclic Voltammetric Recording; Section III: Electrophysiology and Purification of Specific Receptors; Chapter 11: Electrophysiological Expression of Ion Channels in Xenopus Oocytes; Publisher Summary; Introduction; Preparation of RNA; Poly(A)+ Selection.