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Centrifugal separations in molecular and cell biology /
Centrifugal Separations in Molecular and Cell Biology focuses on the application of modern centrifugation technology in molecular and cell biology, including the separation and fractionation of biological particles by centrifugation on the preparative and analytical scales.
| Clasificación: | Libro Electrónico |
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| Otros Autores: | , |
| Formato: | Electrónico eBook |
| Idioma: | Inglés |
| Publicado: |
London ; Boston :
Butterworths,
1978.
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| Temas: | |
| Acceso en línea: | Texto completo |
Tabla de Contenidos:
- Cover image; Title page; Table of Contents; Copyright; Preface; Contributors; Chapter 1: Introduction: Principles and Practices of Centrifugation; Publisher Summary; BASIC CONCEPTS OF SEDIMENTATION THEORY; CENTRIFUGATION METHODS; DESIGNING CENTRIFUGATION EXPERIMENTS; CARE OF CENTRIFUGES AND ROTORS; Chapter 2: The Bases of Centrifugal Separations; Publisher Summary; COMPARISON OF ZONAL CENTRIFUGATION WITH COMPLEMENTARY TECHNIQUES; THEORY OF RATE-ZONAL EXPERIMENTS; ISOPYCNIC ZONAL EXPERIMENTS; STATISTICAL ESTIMATION OF MOLECULAR PARAMETERS; SIMULATION OF ZONAL EXPERIMENTS; APPENDIX.
- Chapter 3: Practical Aspects of Rate-Zonal CentrifugationPublisher Summary; EXPERIMENTAL DESIGN; GRADIENT MATERIALS; RESOLUTION AND GRADIENT SHAPE; LOADING, RUNNING AND ANALYSING GRADIENTS; APPENDIX I DENSITY AND VISCOSITY OF SUCROSE SOLUTIONS; APPENDIX II DENSITY AND VISCOSITY OF GLYCEROL SOLUTIONS; Chapter 4: Fractionations in Zonal Rotors; Publisher Summary; DESIGN AND OPERATION OF BATCH-TYPE ROTORS; FRACTIONATIONS IN BATCH-TYPE ROTORS*; CONTINUOUS-FLOW ZONAL CENTRIFUGATION; CENTRIFUGAL ELUTRIATION; ACKNOWLEDGEMENTS; Chapter 5: Rate-Zonal Centrifugation: Quantitative Aspects.
- Publisher SummarySOME IMPORTANT UNDERLYING ASSUMPTIONS; GUIDE FOR THE COMPUTERIZED CALCULATION OF SEDIMENTATION COEFFICIENTS; GUIDE FOR THE MANUAL CALCULATION OF SEDIMENTATION COEFFICIENTS; ACCURACY OF SEDIMENTATION COEFFICIENTS CALCULATED FROM DENSITY-GRADIENT DATA USING LARGE-SCALE ZONAL ROTORS; OTHER ANALYTICAL USES; ACKNOWLEDGEMENTS; APPENDIX I FORTRAN PROGRAM FOR CALCULATING SEDIMENTATION COEFFICIENTS; APPENDIX II CALCULATING RADIUS-VOLUME RELATIONSHIPS; APPENDIX III SEDIM VALUES FOR VARIOUS PARTICLE DENSITIES; APPENDIX IV CALCULATING THE NATURAL LOGARITHM OF THE ROTOR RADIUS.
- Chapter 6: Isopycnic Centrifugation in Ionic MediaPublisher Summary; BASIC PROCEDURES; OPTIMUM DESIGN OF BUOYANT DENSITY-GRADIENT SEPARATIONS; DATA FROM ISOPYCNIC GRADIENTS; SEPARATIONS BY ISOPYCNIC BANDING; LARGE-SCALE PREPARATIVE PROCEDURES; ACKNOWLEDGEMENTS; Chapter 7: Isopycnic Centrifugation in Non-Ionic Media; Publisher Summary; GENERAL TECHNIQUES; ACKNOWLEDGEMENTS; Chapter 8: Analytical Ultracentrifugation; Publisher Summary; HISTORY AND SCOPE; INSTRUMENTATION; SEDIMENTATION EQUILIBRIUM ANALYSIS; SEDIMENTATION VELOCITY ANALYSIS; CONCLUSIONS.
- Chapter 9: Characteristics of Ultracentrifuge Rotors and TubesPublisher Summary; DESIGNS AND TYPES OF ROTOR; CENTRIFUGE TUBES AND CAPS; DERATING ROTORS; ROUTINE MAINTENANCE OF ROTORS; ACKNOWLEDGEMENTS; APPENDIX: PHYSICAL DIMENSIONS AND CHARACTERISTICS OF ROTORSAPPENDIX: PHYSICAL DIMENSIONS AND CHARACTERISTICS OF ROTORS; Index.