Laboratory methods in microbiology /
Laboratory Methods in Microbiology is a laboratory manual based on the experience of the authors over several years in devising and organizing practical classes in microbiology to meet the requirements of students following courses in microbiology at the West of Scotland Agricultural College. The pr...
Clasificación: | Libro Electrónico |
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Autores principales: | , |
Formato: | Electrónico eBook |
Idioma: | Inglés |
Publicado: |
London ; New York :
Academic Press,
1966.
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Temas: | |
Acceso en línea: | Texto completo |
Tabla de Contenidos:
- Cover image; Title page; Table of Contents; Copyright; PREFACE; Part I: BASIC METHODS; Chapter 1: GENERAL NOTES ON LABORATORY PROCEDURE; Chapter 2: LABORATORY REPORTS; Chapter 3: PROCEDURE FOR THE USE OF A MICROSCOPE WITH AN OIL-IMMERSION OBJECTIVE; Chapter 4: EXAMINATION OF CULTURES FOR MOTILITY BY "HANGING DROP" PREPARATIONS; Chapter 5: STAINING METHODS; Publisher Summary; A Preparation of Smears for Staining; B Simple Stains; C Gram's Staining Method; D Ziehl-Neelsen Method for Staining Acid-fast Bacteria; E Staining of Bacterial Flagella; F The Demonstration of Bacterial Capsules.
- The Wet Indian-ink FilmG Negative Stain; H The Staining of Bacterial Spores; Bartholomew and Mittwer's Spore Staining Method; Chapter 6: CULTIVATION OF MICRO-ORGANISMS; Publisher Summary; A Types of Culture; B Incubation of Cultures; C Method of Inoculation: Aseptic Technique; D Maintenance of Pure Cultures in the Laboratory; Chapter 7: THE DESCRIPTION OF THE MORPHOLOGICAL AND CULTURAL CHARACTERISTICS OF AN ORGANISM; Publisher Summary; A Morphological Characters; B Cultural Characters; Chapter 8: PLATE CULTURES; Publisher Summary; A Preparation of Plates for Streaking.
- B Streak Plates: The Separation of Mixed CulturesChapter 9: DETERMINATION OF THE NUMBER OF VIABLE ORGANISMS IN A SAMPLE; Publisher Summary; A Colony Count Methods; B Membrane Filtration; C Dilution Tube Count; D Dye Reduction Methods; Chapter 10: DETERMINATION OF THE TOTAL NUMBER OF ORGANISMS IN A SAMPLE; Publisher Summary; A The Breed's Smear Method for Direct Microscopic Counts; B Direct Microscopic Counts by Membrane Filtration; C Brown's Opacity Tubes; Chapter 11: STATISTICAL METHODS FOR THE SELECTION AND EXAMINATION OF MICROBIAL COLONIES; Publisher Summary.
- Chapter 12: COMPOSITION OF CULTURE MEDIAPublisher Summary; A Introduction; B Dehydrated Media; C The Determination of the pH of Culture Media; D Examples of Non-selective Culture Media; E Separation of Mixed Cultures-Enrichment Procedures, Elective and Selective Media; F Examples of Selective Culture Media; Chapter 13: METHODS OF ANAEROBIC CULTURE; Publisher Summary; A Robertson's Cooked Meat Medium; B Shake Cultures; C Semi-solid Media; D Vaseline, Paraffin Wax or Agar Seals; E The Anaerobic Jar; Chapter 14: CULTIVATION IN A CARBON DIOXIDE-ENRICHED ATMOSPHERE; Publisher Summary.
- Chapter 15: BIOCHEMICAL TESTS FOR BACTERIAPublisher Summary; A Reactions Involving Protein, Amino Acids and other Nitrogen Compounds, Including Tests for Proteolytic Activity; B Reactions Involving Carbohydrates and other Carbon Compounds, Including Tests for Saccharolytic Activity; C Reactions Involving Fats and Related Substances, Including Tests for Lipolytic Activity; D Miscellaneous Tests; Chapter 16: CLEANING OF GLASSWARE AND APPARATUS; Publisher Summary; A Treatment of New Glassware; B Treatment of Used Glassware and other Apparatus; C Disposable Apparatus; Chapter 17: STERILIZATION.