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A practical guide to membrane protein purification /
A Practical Guide to Membrane Protein Purification is written especially for researchers who have some familarity with separation of water-soluble proteins, but who may not be aware of the pitfalls they face with membrane proteins. This guide presents techniques in a concise form, emphasizing the as...
| Clasificación: | Libro Electrónico |
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| Otros Autores: | , |
| Formato: | Electrónico eBook |
| Idioma: | Inglés |
| Publicado: |
San Diego :
Academic Press,
[1994]
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| Colección: | Separation, detection, and characterization of biological macromolecules.
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| Temas: | |
| Acceso en línea: | Texto completo |
Tabla de Contenidos:
- Front Cover; A Practical Guide to Membrane Protein Purification; Copyright Page; Table of Contents; Contributors; Preface to the Series; Preface to the Volume; Abbreviations; PART I: STRATEGIES AND TECHNIQUES; Chapter 1. Purification Strategies for Membrane Proteins; I. INTRODUCTION; II. GENERAL GUIDE FOR RETAINING CATALYTIC ACTIVITY; III. CHOICE AND SEQUENCE OF PURIFICATION TECHNIQUES; IV. CHOICE OF PROTEIN SOURCE, DISRUPTION OF CELLS, AND PREPARATION OF ORGANELLES AND MEMBRANES; V. PROTEIN ASSAY; VI. SOLUBILIZATION AND STABILIZATION OF MEMBRANE PROTEINS; REFERENCES
- Chapter 2. Chromatographic Techniques and Basic Operations in Membrane Protein PurificationI. INTRODUCTION; II. SOLUBILIZATION-PRECIPITATION; III. CONCENTRATION OF SAMPLES, EXCHANGE OF BUFFER, AND EXCHANGE OF DETERGENT; IV. CHROMATOGRAPHIC TECHNIQUES; REFERENCES; Chapter 3. Denaturing Electrophoretic Techniques; I. INTRODUCTION; II. CHOICE OF OPTIMAL SDS-PAGE SYSTEM AND GEL TYPE; III. TRICINE-SDS-PAGE; IV. COOMASSIE BLUE-SDS-PAGE AND QUANTITATIVE RECOVERY OF LARGE MEMBRANE PROTEINS AND PEPTIDES; V. ELECTROBLOTTING; REFERENCES; Chapter 4. Native Gel Electrophoresis; I. INTRODUCTION
- II. NATIVE ELECTROPHORESIS: TECHNIQUESIII. NATIVE ELECTROPHORESIS: APPLICATIONS; IV. ISOELECTRIC FOCUSING AND TWO-DIMENSIONAL ELECTROPHORESIS; REFERENCES; PART II: DISCUSSION OF SELECTED ISOLATION PROTOCOLS; Chapter 5. Isolation of Complex III, Subcomplexes, and Protein Subunits from Bovine Heart; I. INTRODUCTION; II. PREPARATION OF HYDROXYLAPATITE; III. ISOLATION OF COMPLEX III; IV. DELIPIDATION/ISOLATION OF SUBCOMPLEXES; V. ISOLATION OF PROTEIN SUBUNITS; REFERENCES; Chapter 6. Chromatographic Purification of F1F0-ATPase and Complex I from Bovine Heart Mitochondria; I. INTRODUCTION
- II. PREPARATORY STEPSIII. PURIFICATION OF F1F0-ATPASE; IV. PURIFICATION OF COMPLEX I; V. CONCLUDING REMARKS; REFERENCES; Chapter 7. Isolation of Ubiquinol: Cytochrome c Oxidoreductase (Complex III) from Fungal and Plant Mitochondria; I. INTRODUCTION; II. ISOLATION OF NEUROSPORA CRASSA MITOCHONDRIAL MEMBRANES; III. ISOLATION OF COMPLEX III; IV. RECONSTITUTION OF ISOLATED COMPLEX III; V. APPLICATION OF AFFINITY CHROMATOGRAPHY TO COMPLEX III FROM PLANTS; REFERENCES; Chapter 8. Isolation of Wolinella succinogenes Hydrogenase; I. INTRODUCTION; II. GROWTH OF WOLINELLA SUCCINOGENES
- III. ISOLATION OF HYDROGENASEIV. CHARACTERIZATION OF HYDROGENASE; REFERENCES; Chapter 9. Photosystem I from Cyanobacteria Isolated in Crystallizing Form by Preparative Isoelectric Focusing; I. INTRODUCTION; II. TECHNIQUES; III. APPLICATION: PURIFICATION OF PHOTOSYSTEM I; IV. CONCLUSIONS; REFERENCES; Index