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A practical guide to membrane protein purification /
A Practical Guide to Membrane Protein Purification is written especially for researchers who have some familarity with separation of water-soluble proteins, but who may not be aware of the pitfalls they face with membrane proteins. This guide presents techniques in a concise form, emphasizing the as...
| Clasificación: | Libro Electrónico |
|---|---|
| Otros Autores: | , |
| Formato: | Electrónico eBook |
| Idioma: | Inglés |
| Publicado: |
San Diego :
Academic Press,
[1994]
|
| Colección: | Separation, detection, and characterization of biological macromolecules.
|
| Temas: | |
| Acceso en línea: | Texto completo |
MARC
| LEADER | 00000cam a2200000 i 4500 | ||
|---|---|---|---|
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| 003 | OCoLC | ||
| 005 | 20231120111650.0 | ||
| 006 | m o d | ||
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| 008 | 140627s1994 caua ob 001 0 eng d | ||
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| 019 | |a 1162088374 | ||
| 020 | |a 9780080571720 |q (electronic bk.) | ||
| 020 | |a 0080571727 |q (electronic bk.) | ||
| 020 | |z 0127255508 | ||
| 020 | |z 9780127255507 | ||
| 035 | |a (OCoLC)881853640 |z (OCoLC)1162088374 | ||
| 050 | 4 | |a QP552.M44 |b P73 1994eb | |
| 060 | 4 | |a 1995 E-047 | |
| 060 | 4 | |a QU 25 |b P895 1994 | |
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| 084 | |a WD 5275 |2 rvk | ||
| 084 | |a WE 5000 |2 rvk | ||
| 084 | |a CHE 887f |2 stub | ||
| 084 | |a CHE 820f |2 stub | ||
| 245 | 0 | 2 | |a A practical guide to membrane protein purification / |c edited by Gebhard von Jagow, Hermann Schagger. |
| 264 | 1 | |a San Diego : |b Academic Press, |c [1994] | |
| 264 | 4 | |c �1994 | |
| 300 | |a 1 online resource (xvii, 166 pages) : |b illustrations | ||
| 336 | |a text |b txt |2 rdacontent | ||
| 337 | |a computer |b c |2 rdamedia | ||
| 338 | |a online resource |b cr |2 rdacarrier | ||
| 490 | 1 | |a Separation, detection, and characterization of biological macromolecules | |
| 504 | |a Includes bibliographical references and index. | ||
| 520 | |a A Practical Guide to Membrane Protein Purification is written especially for researchers who have some familarity with separation of water-soluble proteins, but who may not be aware of the pitfalls they face with membrane proteins. This guide presents techniques in a concise form, emphasizing the aspects unique to membrane proteins. The book explains the principles of the methods, permitting researchers and students new to this area to adapt these techniques to their particular needs. The second volume in the series, this book is an essential manual for investigations of structure and function of native membrane proteins, as well as for purification of these proteins for immunization and protein sequencing. Separation, Detection, and Characterization of Biological Macromolecules is a new series of laboratory guides. Each volume focuses on a topic of central interest to scientists and students in biomedical and biological research. Introductory chapters are followed by clear, step-by-step protocols that present principles and practice. These concise manuals are designed for optimal understanding of methods as well as for practical benchtop use. | ||
| 588 | 0 | |a Print version record. | |
| 505 | 0 | |a Front Cover; A Practical Guide to Membrane Protein Purification; Copyright Page; Table of Contents; Contributors; Preface to the Series; Preface to the Volume; Abbreviations; PART I: STRATEGIES AND TECHNIQUES; Chapter 1. Purification Strategies for Membrane Proteins; I. INTRODUCTION; II. GENERAL GUIDE FOR RETAINING CATALYTIC ACTIVITY; III. CHOICE AND SEQUENCE OF PURIFICATION TECHNIQUES; IV. CHOICE OF PROTEIN SOURCE, DISRUPTION OF CELLS, AND PREPARATION OF ORGANELLES AND MEMBRANES; V. PROTEIN ASSAY; VI. SOLUBILIZATION AND STABILIZATION OF MEMBRANE PROTEINS; REFERENCES | |
| 505 | 8 | |a Chapter 2. Chromatographic Techniques and Basic Operations in Membrane Protein PurificationI. INTRODUCTION; II. SOLUBILIZATION-PRECIPITATION; III. CONCENTRATION OF SAMPLES, EXCHANGE OF BUFFER, AND EXCHANGE OF DETERGENT; IV. CHROMATOGRAPHIC TECHNIQUES; REFERENCES; Chapter 3. Denaturing Electrophoretic Techniques; I. INTRODUCTION; II. CHOICE OF OPTIMAL SDS-PAGE SYSTEM AND GEL TYPE; III. TRICINE-SDS-PAGE; IV. COOMASSIE BLUE-SDS-PAGE AND QUANTITATIVE RECOVERY OF LARGE MEMBRANE PROTEINS AND PEPTIDES; V. ELECTROBLOTTING; REFERENCES; Chapter 4. Native Gel Electrophoresis; I. INTRODUCTION | |
| 505 | 8 | |a II. NATIVE ELECTROPHORESIS: TECHNIQUESIII. NATIVE ELECTROPHORESIS: APPLICATIONS; IV. ISOELECTRIC FOCUSING AND TWO-DIMENSIONAL ELECTROPHORESIS; REFERENCES; PART II: DISCUSSION OF SELECTED ISOLATION PROTOCOLS; Chapter 5. Isolation of Complex III, Subcomplexes, and Protein Subunits from Bovine Heart; I. INTRODUCTION; II. PREPARATION OF HYDROXYLAPATITE; III. ISOLATION OF COMPLEX III; IV. DELIPIDATION/ISOLATION OF SUBCOMPLEXES; V. ISOLATION OF PROTEIN SUBUNITS; REFERENCES; Chapter 6. Chromatographic Purification of F1F0-ATPase and Complex I from Bovine Heart Mitochondria; I. INTRODUCTION | |
| 505 | 8 | |a II. PREPARATORY STEPSIII. PURIFICATION OF F1F0-ATPASE; IV. PURIFICATION OF COMPLEX I; V. CONCLUDING REMARKS; REFERENCES; Chapter 7. Isolation of Ubiquinol: Cytochrome c Oxidoreductase (Complex III) from Fungal and Plant Mitochondria; I. INTRODUCTION; II. ISOLATION OF NEUROSPORA CRASSA MITOCHONDRIAL MEMBRANES; III. ISOLATION OF COMPLEX III; IV. RECONSTITUTION OF ISOLATED COMPLEX III; V. APPLICATION OF AFFINITY CHROMATOGRAPHY TO COMPLEX III FROM PLANTS; REFERENCES; Chapter 8. Isolation of Wolinella succinogenes Hydrogenase; I. INTRODUCTION; II. GROWTH OF WOLINELLA SUCCINOGENES | |
| 505 | 8 | |a III. ISOLATION OF HYDROGENASEIV. CHARACTERIZATION OF HYDROGENASE; REFERENCES; Chapter 9. Photosystem I from Cyanobacteria Isolated in Crystallizing Form by Preparative Isoelectric Focusing; I. INTRODUCTION; II. TECHNIQUES; III. APPLICATION: PURIFICATION OF PHOTOSYSTEM I; IV. CONCLUSIONS; REFERENCES; Index | |
| 546 | |a English. | ||
| 650 | 0 | |a Membrane proteins |x Purification. | |
| 650 | 1 | 2 | |a Membrane Proteins |x isolation & purification |0 (DNLM)D008565Q000302 |
| 650 | 7 | |a SCIENCE |x Life Sciences |x Biology. |2 bisacsh | |
| 650 | 7 | |a Membrane proteins |x Purification. |2 fast |0 (OCoLC)fst01015853 | |
| 650 | 7 | |a Biomembran |2 gnd |0 (DE-588)4006884-5 | |
| 650 | 7 | |a Isolierung |g Chemie |2 gnd |0 (DE-588)4122223-4 | |
| 650 | 7 | |a Membranproteine |2 gnd |0 (DE-588)4130026-9 | |
| 650 | 1 | 7 | |a Planten. |2 gtt |
| 650 | 1 | 7 | |a Membranen. |2 gtt |
| 650 | 1 | 7 | |a Eiwitten. |2 gtt |
| 650 | 1 | 7 | |a Zuiveringstechnieken. |2 gtt |
| 650 | 7 | |a Prot�eines membranaires |x Purification. |2 ram | |
| 655 | 2 | |a Laboratory Manual |0 (DNLM)D020484 | |
| 655 | 7 | |a Laboratory manuals. |2 fast |0 (OCoLC)fst01920776 | |
| 655 | 7 | |a Laboratory manuals. |2 lcgft | |
| 655 | 7 | |a Manuels de laboratoire. |2 rvmgf |0 (CaQQLa)RVMGF-000001214 | |
| 700 | 1 | |a Jagow, Gebhard von. | |
| 700 | 1 | |a Schagger, Hermann. | |
| 740 | 0 | |a Membrane protein purification. | |
| 776 | 0 | 8 | |i Print version: |t Practical guide to membrane protein purification |z 0127255508 |w (DLC) 94014643 |w (OCoLC)30110539 |
| 830 | 0 | |a Separation, detection, and characterization of biological macromolecules. | |
| 856 | 4 | 0 | |u https://sciencedirect.uam.elogim.com/science/book/9780080571720 |z Texto completo |