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Enzymatic methods of analysis /
Enzymatic Methods of Analysis attempts to cover all the aspects of modern enzymatic analysis. Enzymes possess a great potential usefulness in analytical chemistry. The specificity of enzymes can solve the primary problem of most analytical chemists-the analysis of one substance in the presence of ma...
| Clasificación: | Libro Electrónico |
|---|---|
| Autor principal: | |
| Formato: | Electrónico eBook |
| Idioma: | Inglés |
| Publicado: |
Oxford ; New York :
Pergamon Press,
[1970]
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| Edición: | First edition]. |
| Colección: | International series of monographs in analytical chemistry ;
v. 34. |
| Temas: | |
| Acceso en línea: | Texto completo |
Tabla de Contenidos:
- Front Cover; Enzymatic Methods of Analysis; Copyright Page; Table of Contents; PREFACE; Dedication; CHAPTER 1. GENERAL CONSIDERATIONS; A. PRINCIPLES OF ENZYMATIC ANALYSIS; B. PROPERTIES OF ENZYMES; C. FACTORS INFLUENCING THE ENZYME REACTION RATE; D. DETERMINATION OF CONCENTRATIONS; E. HANDLING BIOCHEMICAL REAGENTS; F. SOURCES OF REAGENTS; G. ENZYME ACTIVITY; REFERENCES; CHAPTER 2. METHODS OF ASSAY; A. CHEMICAL METHODS; B. INSTRUMENTAL METHODS; REFERENCES; CHAPTER 3. DETERMINATION OF ENZYMES; A. GENERAL; B. HYDROLYTIC ENZYMES; C. OXIDATIVE ENZYMES; D. DEHYDROGENASE ENZYMES.
- E. kinases and transaminasesf. other enzymes; chapter 4. determination of substrates; a. general; b. carbohydrates; c. amines; d. amino acids; e. organic acids; references; chapter 5. determination of activators and coenzymes; a. general; b. determination of inorganic substances; c. determination of coenzymes; references; chapter 6. determination of inhibitors; a. general; b. determination of inorganic substances; c. determination of pesticides; d. determination of other organics; references; chapter 7. the immobilized enzyme; a. general; b. immobilization in starch gel.
- C. immobilization in polyacrylamide gelsd. other methods for immobilizing enzymes; e. commercial availability of immobilized enzymes; f. future applications; references; chapter 8. use of automation in enzymic analysis; a. general; b. initial slope method; c fixed concentration (variable time) methods; d. fixed time method; references; appendix; author index; subject index; other titles in the series in analytical chemistry.