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In vitro toxicity indicators /
About the Series: In the tradition of Methods in Enzymology and Methods in Neurosciences, Academic Press is pleased to announce a new serial: Methods in Toxicology. There is a pressing need among researchers involved in toxicologic investigation for a series of publications that organizes and presen...
| Clasificación: | Libro Electrónico |
|---|---|
| Otros Autores: | , |
| Formato: | Electrónico eBook |
| Idioma: | Inglés |
| Publicado: |
San Diego :
Academic Press,
[1994]
|
| Colección: | Methods in toxicology ;
v. 1B. |
| Temas: | |
| Acceso en línea: | Texto completo |
Tabla de Contenidos:
- Perspectives
- Cellular and molecular pathobiology of reversible and irreversible injury
- Molecular mechanisms of toxic cell death: an overview
- Membrane lipid peroxidation: cellular mechanisms and toxicological implications
- Membrane integrity and function
- Enzyme leakage as an indicator of cytoxicity in cultured cells
- Cellular K
- Morphological and biochemical investigations on plasma membrane blebbing during cell injury
- Morphological evaluation of gap junctional intercellular communication.
- Fluorescence microscopic examination of cytoskeletal elements
- Energy coupled Na cotransport
- Renal organic ion transport
- Endoplasmic reticulum calcium pump
- Energy metabolism
- Measurement of oxygen consumption
- ATP and other energetic parameters as indicators of cell injury
- Evaluation of glucose metabolism in vitro: altered competence of intermediary metabolism as an index of hepatotoxicity
- DNA-related indices
- Quantitation of DNA by fluorometry
- Techniques for measuring cell proliferation.
- Nuclease P1-enhanced ³²P-postlabeling assay for aromatic carcinogen-DNA adducts
- Genomic hypomethylation
- DNA degradation: double-strand breaks
- Genotypic mutation analysis by RFLP/PCR
- Functional indices
- Gluconeogenesis in liver cells
- Measurement of protein synthesis
- Measuring collagen synthesis
- Measurement of macrophage phagocytosis using a sheep red blood cell assay
- Biochemical indices
- Cell function studies using fluorescent Ca² indicators
- Ca²-bioluminescent indicators.
- Measurement of receptor-activated accumulation of inositol phosphates as an index of phospholipase C activity
- Covalent binding to proteins
- Glutathione, oxidized glutathione and mixed disulfides in biological samples
- Lipid peroxidation
- Thiobarbituric acid assay reactants
- Volatile hydrocarbons
- Electronically excited state formation
- Spectrophotometric detection of lipid-conjugated dienes
- Quantitation of conjugated dienes by a dieles-alder reaction
- Quantitation of conjugated dienes by second-derivative UV spectroscopy.
- Specialized techniques
- Centrifuge filtration separation of cells and the measurement of constituents
- Flow cytometry
- Single-cell microscopic techniques for studying toxic injury
- Measurement of macrophage and neutrophil chemotaxis
- Two-dimensional electrophoresis as applied to problems of in vitro and in vivo toxicology.