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Cell-wide metabolic alterations associated with malignancy /
This new volume of Methods in Enzymology continues the legacy of this premier serial with quality chapters authored by leaders in the field. This volume covers research methods providing a theoretical overview on metabolic alterations of cancer cells and a series of protocols that can be employed to...
| Clasificación: | Libro Electrónico |
|---|---|
| Otros Autores: | , |
| Formato: | Electrónico eBook |
| Idioma: | Inglés |
| Publicado: |
Amsterdam ; Boston :
Academic Press,
2014.
|
| Edición: | First edition. |
| Colección: | Methods in enzymology ;
v. 543. |
| Temas: | |
| Acceso en línea: | Texto completo Texto completo |
Tabla de Contenidos:
- Methods to measure cytoplasmic and mitochondrial Ca2+ concentration using Ca2+ -sensitive dyes
- Methods to measure intracellular Ca2+ fluxes with organelle-targeted aequorin-bsaed probes
- Measuring baseline Ca2+ levels iin subcellular compartments using genetically engineered fluorescent indicators
- Autophagy and autophagic flux in tumor cells
- Methods to assess autophagy in situ-transmisson electron microscopy versus immunohistochemistry
- Methods to measure the enzymatic activity of P13Ks
- Luciferase-based reporter to monitor the transciptional activity of the SIRT3 promoter
- Meabolomic profiling of cultured cancer cells
- Pulsed stable isotope-resolved metaolomic studies
- Single-cell imaging for the study of oncometabolism
- Study of cellular oncometabolism via multidimensional protein identification technology
- In vivo quantitative proteomics for the study of oncometabolism
- Metabolomic profiling of neoplastic lesions in mice
- Metabolomic profiling of tumor-bearing mice
- Metabolomic studies of patient material by high-resolution magic angle spinning nuclear magnetic resonance spectroscopy
- Analysis of metabolomic profiling data acquired on GC-MS.