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Cell Structure and Function by Microspectrofluorometry.
Cell Structure and Function by Microspectrofluorometry.
| Clasificación: | Libro Electrónico |
|---|---|
| Autor principal: | |
| Formato: | Electrónico eBook |
| Idioma: | Inglés |
| Publicado: |
Burlington :
Elsevier Science,
1989.
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| Temas: | |
| Acceso en línea: | Texto completo |
Tabla de Contenidos:
- Cover image; Title page; Table of Contents; ANALYTICAL CYTOLOGY SERIES; Copyright; Dedication; Contributors; Preface; Tomas Hirschfeld-In Memoriam; PART I: HISTORY; Chapter 1: The Origins of Modern Fluorescence Microscopy and Fluorescent Probes; I INTRODUCTION; II THE FIRST FLUORESCENCE MICROSCOPES; III TECHNICAL PROGRESS; IV ADVANCES IN BIOMEDICAL APPLICATIONS; V MODERN FLUORESCENCE MICROSCOPY IN CELL AND MOLECULAR BIOLOGY; VI DEVELOPMENT OF IMMUNOFLUORESCENCE; ACKNOWLEDGMENTS; PART II: METHODS; Chapter 2: Microspectroscopy and Flow Cytometry; Publisher Summary.
- Chapter 3: From Solution Spectroscopy to Image SpectroscopyPublisher Summary; I FLUORESCENCE SPECTRA; II FLUORESCENCE EXCITATION SPECTRUM; III FLUORESCENCE LIFETIME AND YIELD; IV FLUORESCENCE POLARIZATION; Chapter 4: High-Resolution Fluorescence and Phase Microscopy in Conjunction with Micromanipulation for in Situ Study of Metabolism in Living Cells; Publisher Summary; I INTRODUCTION; II RESOLUTION OF TRANSMISSION AND FLUORESCENCE MICROSCOPES; III MICROSCOPIC METHODS; IV LONG-WORKING-DISTANCE CONDENSER FOR MICROMANIPULATION; V APPLICATIONS OF SPECTROSCOPY TO FLUORESCENCE MICROSCOPY.
- VI INSTRUMENT DESIGNVII APPLICATION OF PHOTOGRAPHY; VIII FUTURE DEVELOPMENTS; Chapter 5: FRET Microscopy: Digital Imaging of Fluorescence Resonance Energy Transfer. Application in Cell Biology; Publisher Summary; I INTRODUCTION; II THEORY OF FLUORESCENCE RESONANCE ENERGY TRANSFER; III MEASUREMENT OF FRET: DATA ACQUISITION AND ANALYSIS; IV EXPERIMENTAL METHODS AND RESULTS; V DISCUSSION AND FUTURE PROSPECTS; ACKNOWLEDGMENTS; Chapter 6: Fluorescence Scanning Instrumentation; Publisher Summary; I INTRODUCTION; II STAGE SCANNING MICROFLUOROMETERS; III LASER SCANNING MICROFLUOROMETERS.
- IV COMPOSITION OF A LASER SCANNING MICROSCOPEV CONFOCAL LASER SCANNING; VI CHARACTERISTICS OF LASER SCANNING; VII APPLICATIONS IN LASER SCANNING; ACKNOWLEDGMENTS; Chapter 7: Fluorescence Microscopy in Three Dimensions: Microtomoscopy; Publisher Summary; I INTRODUCTION; II CONFOCAL MICROSCOPY; III APPLICATIONS; IV DISCUSSION; ACKNOWLEDGMENTS; Chapter 8: Fluorescence Photochemical Techniques for the Study of Transport in Cytoplasm and Cytoplasmic Models; Publisher Summary; I INTRODUCTION; II APPARATUS AND METHODOLOGY; III APPLICATIONS TO CYTOPLASMIC TRANSPORT.
- IV APPLICATIONS TO CYTOPLASMIC MODELS IN VITROV FLUORESCENCE PHOTOACTIVATION; VI CONCLUSIONS; ACKNOWLEDGMENT; Chapter 9: Principles of Frequency-Domain Fluorescence Spectroscopy and Applications to Protein Fluorescence; Publisher Summary; I INTRODUCTION; II COMPARISON OF TIME- AND FREQUENCY-DOMAIN MEASUREMENTS; III THEORY OF FREQUENCY-DOMAIN FLUOROMETRY; IV TRYPTOPHAN FLUORESCENCE FROM PROTEINS; V 2-GHz FREQUENCY-DOMAIN FLUOROMETRY; VI ADDITIONAL APPLICATIONS OF FREQUENCY-DOMAIN FLUOROMETRY; VII FUTURE DEVELOPMENTS; ACKNOWLEDGMENTS; Chapter 10: The First Picosecond in Vision.