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Laboratory methods in enzymology. Volume 541, Protein, Part C /
In this volume we have brought together a number of core protocols concentrating on Protein, carefully written and edited by experts.
| Clasificación: | Libro Electrónico |
|---|---|
| Otros Autores: | |
| Formato: | Electrónico eBook |
| Idioma: | Inglés |
| Publicado: |
Amsterdam :
Elsevier/Academic Press,
2014.
|
| Colección: | Methods in enzymology ;
volume 541. |
| Temas: | |
| Acceso en línea: | Texto completo Texto completo |
MARC
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| 245 | 0 | 0 | |a Laboratory methods in enzymology. |n Volume 541, |p Protein, |n Part C / |c edited by Jon Lorsch. |
| 246 | 3 | |a Protein | |
| 264 | 1 | |a Amsterdam : |b Elsevier/Academic Press, |c 2014. | |
| 300 | |a 1 online resource (xvii, 272 pages) : |b illustrations (black and white) | ||
| 336 | |a text |b txt |2 rdacontent | ||
| 337 | |a computer |b c |2 rdamedia | ||
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| 490 | 1 | |a Methods in enzymology, |x 0076-6879 ; |v volume 541 | |
| 504 | |a Includes bibliographical references and index. | ||
| 520 | |a In this volume we have brought together a number of core protocols concentrating on Protein, carefully written and edited by experts. | ||
| 505 | 0 | |a Front Cover; Laboratory Methods in Enzymology: Protein Part C; Copyright; Contents; Contributors; Miscellaneous; Preface; Section I: Protein Protocols/Protein Precipitation; Chapter One: TCA Precipitation; 1. Theory; 2. Equipment; 3. Materials; 3.1. Solutions and buffers; 4. Protocol; 4.1. Preparation; 4.2. Duration; 4.3. Caution; 5. Step 1A Trichloroacetic Acid Precipitation; 5.1. Overview; 5.2. Duration; 5.3. Tip; 5.4. Tip; 5.5. Tip; 6. Step 1B Deoxycholate-Trichloroacetic Acid Precipitation; 6.1. Overview; 6.2. Duration; 6.3. Tip; 6.4. Tip; 6.5. Tip; References. | |
| 505 | 8 | |a Referenced Protocols in Methods NavigatorSection II: Protein Protocols/Protein Pull-Down Methods; Chapter Two: Coimmunoprecipitation of Proteins from Yeast; 1. Theory; 2. Equipment; 3. Materials; 3.1. Solutions and buffers; 4. Protocol; 4.1. Duration; 4.2. Preparation; 5. Step 1 Preparation of Whole Cell Lysates; 5.1. Overview; 5.2. Duration; 6. Step 2 Normalization of Cell Lysates; 6.1. Overview; 6.2. Duration; 6.3. Tip; 7. Step 3 Coimmunoprecipitation; 7.1. Overview; 7.2. Duration; 7.3. Tip; 7.4. Tip; 7.5. Tip; 7.6. Tip; 7.7. Tip; 7.8. Tip; 7.9. Note. | |
| 505 | 8 | |a 8. Step 4 Wash and Elute the Immunoprecipitates8.1. Overview; 8.2. Duration; 8.3. Tip; 8.4. Tip; 9. Step 5 Analysis of Immunoprecipitations; 9.1. Overview; 9.2. Duration; References; Referenced Literature; Related Literature; Referenced Protocols in Methods Navigator; Chapter Three: Coupling Antibody to Cyanogen Bromide-Activated Sepharose; 1. Theory; 2. Equipment; 3. Materials; 3.1. Solutions and buffers; 4. Protocol; 4.1. Duration; 4.2. Preparation; 5. Step 1 Preparation of Antibody and Resin; 5.1. Overview; 5.2. Duration; 5.3. Tip; 5.4. Tip; 5.5. Tip; 5.6. Tip. | |
| 505 | 8 | |a 6. Step 2 Coupling the Antibody to the Resin6.1. Overview; 6.2. Duration; 6.3. Tip; 7. Step 3 Quench the Reaction; 7.1. Overview; 7.2. Duration; 7.3. Tip; 8. Step 4 Wash the Resin; 8.1. Overview; 8.2. Duration; References; Referenced Protocols in Methods Navigator; Chapter Four: Analysis of Protein-Protein Interactions by Coimmunoprecipitation; 1. Theory; 2. Equipment; 3. Materials; 3.1. Solutions and buffers; 4. Protocol; 4.1. Preparation; 4.2. Duration; 5. Step 1 Isolation of the Protein of Interest by Immunoprecipitation; 5.1. Overview; 5.2. Duration; 5.3. Tip; 5.4. Tip; 5.5. Tip. | |
| 505 | 8 | |a 6. Step 2 Detection of the Binding Partner by Immunoblotting6.1. Overview; 6.2. Duration; 6.3. Tip; 6.4. Tip; References; Referenced Literature; Referenced Protocols in Methods Navigator; Section III: Protein Protocols/Protein Purification; Chapter Five: Use and Application of Hydrophobic Interaction Chromatography for Protein Purification; 1. Theory; 1.1. Latest technology in HIC adsorbents; 1.2. Advantages and disadvantages of using HIC; 2. Equipment; 3. Materials; 3.1. Solutions and buffers; 3.2. Preparation; 4. Protocol; 4.1. Preparation; 4.2. Duration; 5. Step 1 Column Equilibration. | |
| 650 | 0 | |a Proteins |x Analysis. | |
| 650 | 0 | |a Proteins |x Purification. | |
| 650 | 6 | |a Prot�eines |x Analyse. |0 (CaQQLa)201-0031951 | |
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| 650 | 7 | |a Proteins |x Purification |2 fast |0 (OCoLC)fst01079750 | |
| 700 | 1 | |a Lorsch, Jon, |e editor. | |
| 776 | 0 | 8 | |i Print version: |a Lorsch, Jon. |t Laboratory Methods in Enzymology. |d Academic Press 2014 |z 1306540887 |
| 830 | 0 | |a Methods in enzymology ; |v volume 541. |x 1557-7988 | |
| 856 | 4 | 0 | |u https://sciencedirect.uam.elogim.com/science/book/9780124201194 |z Texto completo |
| 856 | 4 | 0 | |u https://sciencedirect.uam.elogim.com/science/bookseries/00766879/541 |z Texto completo |