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Applications of advanced omics technologies : from genes to metabolites /
The book contains contributions concerning the application of the new instrumental and methodological developments in omics technologies, including those related to Genomics, Transcriptomics, Proteomics, Peptidomics and Metabolomics, Lipidomics and Foodomics. The16 chapters discuss in detail: innova...
| Clasificación: | Libro Electrónico |
|---|---|
| Otros Autores: | , , |
| Formato: | Electrónico eBook |
| Idioma: | Inglés |
| Publicado: |
Kidlington, Oxford, UK :
Elsevier,
2014.
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| Colección: | Wilson and Wilson's comprehensive analytical chemistry ;
v. 64. |
| Temas: | |
| Acceso en línea: | Texto completo Texto completo |
Tabla de Contenidos:
- Front Cover; Applications of Advanced Omics Technologies: From Genes to Metabolites; Copyright; Dedication; Contents; Contributors to Volume 64; Series Editor�s Preface; Preface; Part I: Genomics and Transcriptomics; Chapter 1: Applications of Functional Gene Microarrays for Profiling Microbial Communities; 1. Introduction; 2. Comparison of Microarrays with High-Throughput Sequencing; 3. Functional Gene Arrays; 3.1. GeoChip Microarrays; 3.2. Other FGAs; 3.2.1. Hydrogenase Chip; 3.2.2. Food Safety and Health Applications; 3.2.3. Detection of Novel Sequences.
- 3.2.4. Other Microarrays: Unique Applications4. Key Issues in Microarray Application; 5. Future Advancements; 6. Conclusions; Acknowledgments; References; Chapter 2: microRNA Profiling: An Overview of Current Technologies and Applications; 1. Introduction; 2. miRNA Biogenesis and Nomenclature; 3. Considerations for miRNA Profiling; 4. miRNA Extraction; 5. qPCR Analysis of miRNAs; 6. Microarray Profiling of miRNAs; 7. Small RNA-seq; 8. In Silico miRNA Analysis Resources; 9. miRNA Functional Analysis; 10. Recent Developments in the Application of miRNA Profiling to Cancer Research.
- 11. ConclusionsReferences; Chapter 3: Application of Novel Genotyping Microarray Technologies in Cancer Research; 1. Introduction; 2. Cancer Genetics: From Biology to Clinical Practice; 2.1. The Discovery of Cancer Genetics; 2.2. The Introduction of Cancer Genetics on Cancer Therapy; 2.3. Tumor Genome Analysis Applied to Improve Cancer Therapy; 3. Genotyping Technologies; 3.1. The Study of Defined Variants at the Level of DNA; 3.1.1. Variants Discovery by Sequencing; 3.1.2. Alternative Techniques for the Analysis of Defined Variants; 3.1.3. Real-Time PCR-Based Techniques.
- 3.1.4. Whole-Genome SNP Analysis3.2. Analysis of DNA Variation Independent from Single Nucleotide Polymorphisms; 3.3. Variants Found in Small Numbers; 3.4. Gene Expression Studies; 4. Next-Generation Genomic Techniques; 5. Applications of Novel SNP Technologies in Cancer Research; 6. Conclusions and Future Directions; References; Chapter 4: Applications of Next-Generation Sequencing Technologies to the Study of the Human Microbiome; 1. Introduction; 1.1. 16S Sequencing Technologies; 2. Next-Generation Sequencing; 2.1. Roche 454 Pyrosequencing; 2.1.1. Barcoding; 2.1.2. Sequencing Parameters.
- 2.2. Illumina SBS Platform (Genome Analyzer IIx, HiSeq, MiSeq)2.3. AB (Applied Biosystems) SOLiD Platform (AB 5500, 5500xl); 2.4. Pacific Bioscience SMRT Platform (PacBio RS II); 2.5. Ion Torrent (PostLightTM) Semiconductor Platform (Ion PGM, Ion Proton); 3. Bioinformatics; 3.1. Denoising; 3.2. Detecting Chimeras; 3.3. Operational Taxonomic Units; 3.4. Sequence Alignment; 3.5. Phylogenetic Analysis; 3.6. Estimating Diversity; 3.7. Comparing Between Communities; 3.8. Unifying Platforms; 4. The Microbiome in Health and Disease; 4.1. The Gastric Microbiome.