Cargando…
Basic molecular protocols in neuroscience : tips, tricks, and pitfalls /
Basic Neuroscience Protocols: Tips, Tricks, and Pitfalls contains explanatory sections that describe the techniques and what each technique really tells the researcher on a scientific level. These explanations describe relevant controls, troubleshooting, and reaction components for some of the most...
| Clasificación: | Libro Electrónico |
|---|---|
| Autor principal: | |
| Formato: | Electrónico eBook |
| Idioma: | Inglés |
| Publicado: |
Burlington :
Elsevier Science,
2014.
|
| Temas: | |
| Acceso en línea: | Texto completo |
Tabla de Contenidos:
- Front Cover; Basic Molecular Protocols in Neuroscience; Copyright Page; Contents; Introduction; 1 General Notes; Aseptic Technique; Aliquoting; 2 DNA and RNA Extraction Protocols; Phenol:Chloroform (TRIzolTM) Extraction Notes; Phenol:Chloroform Extraction Protocol; RNA Extraction; DNA Extraction; Protein Extraction; Kit Extraction of RNA Notes; Solution Recipes; 3 Agarose Gel Electrophoresis; Electrophoresis Notes; Agarose Gel Electrophoresis Protocol; Solution Recipes; 4 Reverse Transcription (RT) and Polymerase Chain Reaction (PCR); Reverse Transcription Notes
- Polymerase Chain Reaction (PCR) NotesEnd-Point PCR; Primer Design; Reverse-Transcription PCR (RT-PCR); Quantitative PCR (Real-Time PCR or qPCR); Quantitation Methods; Running a Plate and Data Analysis; PCR Controls; PCR Troubleshooting; 5 Antibodies and Titrations; 6 Protein Extraction; Protein Extraction Notes; Total Cytosolic/Membrane Protein Extraction Protocol; Subcellular Fractionation Protocol (Adapted from Two Papers); Subcellular Fractionation Protocol (Detergents); Fractionation, Enriched in Neurons and Glia; Fractionation of P1 Pellet (Microvessel-Enriched Fraction)
- Solution Recipes7 Immunoblotting (Western Blot); Immunoblotting Notes; SDS-PAGE and Immunoblot Protocols; SDS-PAGE and Membrane Transfer Protocol; Immunoblotting Protocol; Fluorescence; Chemiluminescence; PVDF Blot Stripping Protocol; Blot Stripping Protocol (Alternative Stripping Buffer); Coomassie Blue Protocol; Fast Green/Ponceau S Protocol; Dot Blot Protocol; Immunoblot Controls; Immunoblot Troubleshooting; Solution Recipes; 8 Immunoprecipitation; Immunoprecipitation Notes; IP Protocol; IP Controls; IP Troubleshooting; Solution Recipes; 9 Perfusion and Immersion Fixation
- Perfusion Fixation NotesTissue Fixation; Tissue Slicing and Storage; Immersion Fixation Protocol; Cell Culture (Grown on Coverslips); Extracted Tissue; Perfusion Fixation Protocol; Sample Storage; Solution Recipes; 10 Immunohistochemistry; Immunohistochemistry Notes; Types of IHC Staining; Secondary Antibodies and Blocking Buffers; Amplification of the Signal; Multiple Labeling; Antibody Titration Protocol (Ni-DAB); Antibody Titration Protocol (Immunofluorescence); IHC Protocol; Cresyl Violet Counterstain (Nissl Stain) Protocol; IHC Controls; IHC Troubleshooting; Solution Recipes
- 11 In Situ HybridizationIn Situ Hybridization Notes; Types of ISH Probe Labeling; Visualizing Your ISH; ISH Probe Design for Oligonucleotide and PCR Probes; ISH Protocol (Developed from Two Sources); ISH Controls; Solution Recipes; References