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Laboratory methods in enzymology. Volume 539, Protein, Part B /
Laboratory Methods in Enzymology: Protein Part B brings together a number of core protocols concentrating on protein, carefully written and edited by experts. Indispensable tool for the researcher Carefully written and edited by experts to contain step-by-step protocolsIn this volume we have brought...
| Clasificación: | Libro Electrónico |
|---|---|
| Otros Autores: | |
| Formato: | Electrónico eBook |
| Idioma: | Inglés |
| Publicado: |
San Diego, California :
Academic Press,
2014.
|
| Colección: | Methods in enzymology ;
volume 539, pt. B. |
| Temas: | |
| Acceso en línea: | Texto completo Texto completo |
Tabla de Contenidos:
- In vitro synthesis of proteins in bacterial extracts
- Preparation of a Saccharomyces cerevisiae cell-free extract for in vitro translation
- Yeast two-hybrid screen
- UV cross-linking of interacting RNA and protein in cultured cells
- Analysis of RNA-protein interactions by cell mixing
- General protein-protein cross-linking
- Chromatin immunoprecipitation and multiplex sequencing (ChIP-Seq) to identify global transcription factor binding sites in the nematode Caenorhabditis elegans
- PAR-CLIP (phoactivatable ribonucleoside-enhanced crosslinking and immunoprecipitation): a step-by-step protocol to the transcriptome-wide identification of binding sites of RNA-binding proteins
- Determining the RNA specificity and targets of RNA-binding proteins using a three-hybrid system
- Dissecting a known RNA-protein interaction using a yeast three-hybrid system
- Identifying proteins that bind a known RNA sequence using the yeast three-hybrid system.