Cargando…

Enzymes : biochemistry, biotechnology and clinical chemistry /

In recent years, there have been considerable developments in techniques for the investigation and utilisation of enzymes. With the assistance of a co-author, this popular student textbook has been updated to include techniques such as membrane chromatography, aqueous phase partitioning, engineering...

Descripción completa

Detalles Bibliográficos
Clasificación:Libro Electrónico
Autores principales: Palmer, Trevor, 1944- (Autor), Bonner, Philip L. R. (Autor)
Formato: Electrónico eBook
Idioma:Inglés
Publicado: Oxford : Woodhead Publishing, [2011]
Edición:Second edition.
Temas:
Acceso en línea:Texto completo

MARC

LEADER 00000cam a2200000 i 4500
001 SCIDIR_ocn871174123
003 OCoLC
005 20231120111527.0
006 m o d
007 cr cnu---unuuu
008 140227s2011 enka ob 001 0 eng d
040 |a N$T  |b eng  |e rda  |e pn  |c N$T  |d E7B  |d OPELS  |d EBLCP  |d YDXCP  |d DEBSZ  |d OCLCQ  |d MERUC  |d D6H  |d OTZ  |d OCLCQ  |d S2H  |d OCLCO  |d OCLCQ  |d YDXIT  |d OCLCO  |d OCLCQ  |d OCLCO 
019 |a 871224731 
020 |a 9780857099921  |q electronic book 
020 |a 0857099922  |q electronic book 
020 |z 9781904275275 
020 |z 1904275273 
020 |z 9788176710596 
020 |z 8176710598 
035 |a (OCoLC)871174123  |z (OCoLC)871224731 
050 4 |a QP601  |b .P35 2011 
072 7 |a SCI  |x 008000  |2 bisacsh 
082 0 4 |a 572.7  |2 23/eng/20220603 
082 0 4 |a 574.1925  |2 22 
100 1 |a Palmer, Trevor,  |d 1944-  |e author. 
245 1 0 |a Enzymes :  |b biochemistry, biotechnology and clinical chemistry /  |c Trevor Palmer, Philip L. Bonner. 
250 |a Second edition. 
264 1 |a Oxford :  |b Woodhead Publishing,  |c [2011] 
300 |a 1 online resource 
336 |a text  |b txt  |2 rdacontent 
337 |a computer  |b c  |2 rdamedia 
338 |a online resource  |b cr  |2 rdacarrier 
504 |a Includes bibliographical references and index. 
505 0 |a pt. 1. Structure and function of enzymes. An introduction to enzymes ; The structure of proteins ; The biosynthesis and properties of proteins ; Specificity of enzyme action ; Monomeric and oligomeric enzymes -- pt. 2. Kinetic and chemical mechanisms of enzyme-catalysed reactions. An introduction to bioenergetics, catalysis and kinetics ; Kinetics of single-substrate enzyme-catalysed reactions ; Enzyme inhibition ; Kinetics of multi-substrate enzyme-catalysed reactions ; The investigation of active site structure ; The chemical nature of enzyme catalysis ; The binding of ligands to proteins ; Sigmoidal kinetics and allosteric enzymes ; The significance of sigmoidal behaviour -- pt. 3. Application of enzymology. Investigation of enzymes in biological preparations ; Extraction and purification of enzymes ; Enzymes as analytical reagents ; Instrumental techniques available for use in enzymatic analysis ; Applications of enzymatic analysis in medicine, forensic science and industry ; Biotechnological applications of enzymes ; Genomics, proteomics and bioinformatics. 
520 |a In recent years, there have been considerable developments in techniques for the investigation and utilisation of enzymes. With the assistance of a co-author, this popular student textbook has been updated to include techniques such as membrane chromatography, aqueous phase partitioning, engineering recombinant proteins for purification and due to the rapid advances in bioinformatics/proteomics, a discussion of the analysis of complex protein mixtures by 2D-electrophoresis and RPHPLC prior to sequencing by mass spectroscopy. Written with the student firmly in mind, no previous knowledge of biochemistry, and little of chemistry, is assumed. It is intended to provide an introduction to enzymology, and a balanced account of all the various theoretical and applied aspects of the subject which are likely to be included in a course. Provides an introduction to enzymology and a balanced account of the theoretical and applied aspects of the subjectDiscusses techniques such as membrane chromatography, aqueous phase partitioning and engineering recombinant proteins for purificationIncludes a discussion of the analysis of complex protein mixtures by 2D-electrophoresis and RPHPLC prior to sequencing by mass spectroscopy. 
588 |a Description based on online resource; title from digital title page (viewed on June 03, 2022). 
650 0 |a Enzymes. 
650 6 |a Enzymes.  |0 (CaQQLa)201-0002557 
650 7 |a enzyme.  |2 aat  |0 (CStmoGRI)aat300212647 
650 7 |a SCIENCE  |x Life Sciences  |x Biology.  |2 bisacsh 
650 7 |a Enzymes  |2 fast  |0 (OCoLC)fst00913605 
700 1 |a Bonner, Philip L. R.,  |e author. 
776 0 8 |i Print version:  |a Palmer, Trevor, 1944-  |t Enzymes.  |b Second edition /.  |z 9781904275275  |w (DLC) 2008399376  |w (OCoLC)192026804 
856 4 0 |u https://sciencedirect.uam.elogim.com/science/book/9781904275275  |z Texto completo