Tabla de Contenidos:
  • Cover image; Title page; Table of Contents; Copyright page; Contributors to Volume 3; Preface; Methods in Neurosciences; Section I: Autoradiography; Chapter 1: Autoradiographic and Microchemical Methods for Quantitation of Steroid Receptors; Publisher Summary; Introduction; Microchemical Measurement of Steroid Receptors in the Central Nervous System; Quantitative Autoradiography for Measurement of Steroid Receptor Levels in Central Nervous System; Conclusions and Prospectus; Chapter 2: High-Resolution Autoradiographic Mapping of Drug and Hormone Receptors; Publisher Summary
  • Thaw-Mount and Dry-Mount Autoradiography with Thin Frozen SectionsApposition (Sandwich) Techniques; Conclusions; Chapter 3: High-Resolution Autoradiographic Imaging of Brain Activity Patterns with Radiolabeled 2-Deoxyglucose and Glucose; Publisher Summary; Preparation of Rats for Intravenous Injection of Deoxy[14C]glucose or [14C]glucose; High-Resolution Autoradiography at Regional Topographic Level; Comparison of Radiolabeled 2-Deoxyglucose and Glucose as Metabolic Indicators and Consideration of Time Course for 2-Deoxyglucose Studies; Dry-Mount Autoradiography for Cellular Resolution
  • Quantitative Considerations in 2-Deoxyglucose StudiesSummary and Conclusions; Chapter 4: Double- and Single-Label Quantitative Autoradiography for Cerebral Physiology; Publisher Summary; Quantitative Autoradiography: The Basics; Physiological Quantitative Autoradiography; Digital Image Analysis for Double-Label Quantitative Autoradiography; Double-Label Techniques; Summary and Conclusion; Chapter 5: Combination of Tritiated Thymidine Autoradiography and Neuropeptide Immunocytochemistry to Determine Birthdates and Migration Routes of Luteinizing Hormone-Releasing Hormone Neurons
  • Publisher SummaryIntroduction; Preparation of Animals for Autoradiography; Preparation of Paraffin Sections; Immunocytochemical Procedures; Controls for Immunocytochemistry; Autoradiography; Controls for Autoradiography; Section II: Quantification of Structures: Statistical and Computational Methods; Chapter 6: Techniques and Technology for Dynamic Video Imaging of Cellular Fluorescence; Publisher Summary; Introduction; Chemical Probes for Cellular Function; Digital Video Imaging Technology; Biological Results of Dynamic Video Imaging; Comparison of Imaging with Photometric Systems
  • Limitations and ArtifactsFuture Developments; Chapter 7: Three-Dimensional Computer Reconstruction of Perforated Synapses; Publisher Summary; Introduction; Computer-Assisted Three-Dimensional Reconstruction; Study of Perforated Synapses; Results and Conclusions; Chapter 8: Determination of Numerical Density of Perforated and Nonperforated Synapses; Publisher Summary; Introduction; Disector as Sampling Probe; Disector Method in Synaptic Studies; Numerical Density of Synapses and Perforated Synapses; Results and Conclusions