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Microbial Synthetic Biology /
The 40th volume of Methods in Microbiology focuses on microbial synthetic biology. Synthetic biology is a rapidly growing discipline that builds on well-established principles of genetic engineering and biotechnology by integrating computational and engineering approaches to the design and construct...
| Clasificación: | Libro Electrónico |
|---|---|
| Otros Autores: | , |
| Formato: | Electrónico eBook |
| Idioma: | Inglés |
| Publicado: |
Amsterdam [Netherlands] ; Boston :
Elsevier/AP,
2013.
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| Edición: | First edition. |
| Colección: | Methods in microbiology ;
v. 40. |
| Temas: | |
| Acceso en línea: | Texto completo |
Tabla de Contenidos:
- Front Cover; Microbial Synthetic Biology; Copyright; Contents; Contributors; Preface; Chapter 1: Computational Intelligence in the Design of Synthetic Microbial Genetic Systems; 1. Introduction; 2. Computational Infrastructure for Synthetic Biology; 2.1. Biological parts; 2.2. Circuit design and simulation; 2.2.1. Bottom-up versus top-down design; 2.2.2. Computer-aided design; 2.2.3. Standard virtual parts; 2.3. Exploring design space; 3. Computational Intelligence; 3.1. Evolutionary algorithms; 3.1.1. Modularity in evolutionary design; 3.1.2. Simulated annealing
- 3.1.3. The practice and promise of EAs for synthetic biology3.2. Other CI techniques; 3.2.1. Neural networks; 3.2.2. Fuzzy systems; 3.2.3. Swarm intelligence; 4. Discussion; References; Chapter 2: Constraints in the Design of the Synthetic Bacterial Chassis; 1. Introduction; 2. Top-Down Versus Bottom-Up Framing; 3. An Unlimited List of Functions (Organised Starting from the Cell's Structure); 3.1. Compartmentalisation and shaping: the cell's casing; 3.1.1. Interface with the environment; 3.1.2. The need for a casing or a skeleton; 3.2. Information transfer; 3.2.1. Synthesis of macromolecules
- 3.2.2. Regulation3.2.3. Memory; 3.3. Metabolism; 4. Perspective: The Fourth Dimension (When Time Measures and Shapes); Acknowledgements; References; Chapter 3: Social Dimensions of Microbial Synthetic Biology; 1. Introduction: Making Space for a New Discussion; 2. From Implications to Dimensions; 2.1. Metaphors and analogies; 3. From Speculation to Anticipation; 4. From Public Acceptance to Public Good; 4.1. Synthetic biology for a particular purpose-the arsenic biosensor; 4.2. The public good; 5. From Regulation to Governance; 5.1. Responsible research and innovation; Conclusions; References
- Chapter 4: Bacillus subtilis: Model Gram-Positive Synthetic Biology Chassis1. Introduction; 2. The B. subtilis Genome; 2.1. General features; 2.2. Genome annotation; 3. Genome Management and Analysis of Gene Function; 3.1. Gene transfer and recombination; 3.2. Transformation; 3.3. Conjugation; 3.4. Plasmid-based vector systems; 3.5. Special-purpose vectors; 3.6. Expression vectors; 3.7. Genome minimalisation; 4. Analysis of the Transcriptome; 4.1. Transcription and transcription profiling; 4.2. Sigma factors; 4.3. Transcription termination; 4.4. Reporter gene technology
- 4.5. Reporter gene libraries/live cell arrays4.6. Analysis of populations and single cells; 5. Analysis of the Proteome; 6. Analysis of the Metabolome; 7. Parts, Devices, Systems and Applications; 7.1. Parts, systems and devices; 7.2. Engineering genomes with B. subtilis; 7.3. Biosensors; 8. Computational Tools and Resources; Summary; References; Chapter 5: Engineering Microbial Biosensors; 1. Introduction; 2. Areas of Application; 2.1. What to target?; 2.2. Finding potential sensors; 3. Types of Biosensors; 3.1. Response characteristics; 4. Reporters; 4.1. Fluorescence; 4.2. Bioluminescence