The guide to investigation of mouse pregnancy /
"The Guide to Investigation of Mouse Pregnancy is the first publication to cover the mouse placenta or the angiogenic tree the mother develops to support the placenta. This much-needed resource covers monitoring of the cardiovascular system, gestational programming of chronic adult disease, epi...
Clasificación: | Libro Electrónico |
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Otros Autores: | , , , |
Formato: | Electrónico eBook |
Idioma: | Inglés |
Publicado: |
Amsterdam :
Elsevier/Academic Press,
[2014]
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Temas: | |
Acceso en línea: | Texto completo |
Tabla de Contenidos:
- Part I: Atlas. The cycling and pregnant mouse: gross anatomy
- Implant site dissections
- Microscopic anatomy of the pregnant mouse uterus throughout gestation
- Anatomy of the mouse placenta throughout gestation
- Whole-mount immunohistochemistry of early implantation sites
- Magnetic resonance imaging of the mouse placenta
- Part II: Basic biology. Reproductive tract changes during the mouse estrous cycle
- First cell fate decisions in early development: towards establishment of the trophoblast lineage
- Tetraploid complementation assay
- Biology of the ectoplacental cone
- Decidualization and endometrial extracellular matrix remodeling
- Postimplantation development of the chorioallantoic placenta
- Unique features of endometrial dynamics during pregnancy
- Pathology analysis of the placenta
- Changes in structures and relationships of trophoblasts, leukocytes, and maternal vessels in implantation sites to midpregnancy
- The uteroplacental, fetoplacental, and yolk sac circulations in the mouse
- Physiological exchange across mouse placenta
- Hypoxia-associated regulation within implantation sites
- Leukocyte population dynamics and functions at the maternal--fetal interface
- In vivo tracking of mononuclear cells in the virgin uterus and in implantation sites
- Microchimerism in mouse pregnancy
- Systems biology analyses of the placenta
- Contribution of placental genomic imprinting and identification of imprinted genes
- Epigenetics of placental development and function
- Genome-wide analysis of the mouse deciduoma
- Conditional gene deletion in the placenta using the Cre-loxP system
- Genetic engineering of mice to investigate uterine function in early pregnancy
- Lentivirus-mediated transduction of optical reporter genes in blastocysts for placental studies
- Blood pressure regulation during mouse pregnancy
- Intravital imaging of vasoactivity in the uterine arterial tree during pregnancy
- Multimodal imaging of the mouse placenta
- Regulation of parturition
- Cervical changes accompanying birth
- Peri-partum changes to mouse pubic symphysis
- Mammary gland morphogenesis and function during pregnancy
- Gestational programming of the cardiovascular system
- Maternal stress-mediated gene expression changes in the mouse placenta: a mosaic hypothesis of epigenesis
- Pregnancy alterations from environmental pollutants
- Part III: Protocols. Monitoring mouse estrous cycles
- Lentivirus transduction of gene tags in blastocysts
- Tetraploid complementation assay
- Pseudopregnant bead-induced mouse deciduoma model
- Ectoplacental cone isolation, culture and assessment
- Layer-enriched tissue dissection of the mouse placenta in late gestation
- Protocols for placental histology
- Best practice for quantifying the microscopic structure of mouse placenta: the stereological approach
- Electron microscopy and immunoelectromicroscopy protocols
- Laser capture microdissection
- Immunofluorescence and immunohistochemistry
- Nonradioactive in situ hybridization: optimization for tissue sections from pregnant uteri and placenta during the first half of pregnancy
- Whole mount in situ immunohistochemistry
- Guidelines for evaluation of reproductive and developmental endpoints
- Flow cytometric analysis of myometrial and decidual cell suspensions
- Uterine leukocyte isolation and culture
- Scanning electron microscopy and micro-computed tomography imaging of the utero- and fetoplacental circulations
- Radiotelemetry in mouse pregnancy
- Ex vivo perfusion of the mouse placenta for maternal--fetal interaction studies during pregnancy
- Evaluation of placental transport function
- Hypoxia-associated regulation within implantation sites
- Method to locate the uterine artery in mice for micro-ultrasound doppler blood velocity examination
- Two-photon intravital microscopy of the virgin and pregnant mouse uterus
- Intravital imaging of vasoactivity in the uterine arterial vasculature tree during pregnancy
- MRI, intra-vital, and ex-vivo fluorescence microscopy of the mouse uterine vasculature and placenta
- Assessment of changes in the peripartum cervix
- Pubic symphysis evaluation
- Labor and preterm labor
- Epithelial transplantation into the cleared mammary fat pad
- Dietary and hypoxic protocols that alter placental gene expression in response to maternal stress
- Parental-specific gene expression and epigenetic analyses of imprinted genes in mouse placenta
- A method for obtaining epigenomic data
- Methods for genetic engineering in mice
- Appendix: reproductive parameters of common, commercially available mouse strains.