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The guide to investigation of mouse pregnancy /

"The Guide to Investigation of Mouse Pregnancy is the first publication to cover the mouse placenta or the angiogenic tree the mother develops to support the placenta. This much-needed resource covers monitoring of the cardiovascular system, gestational programming of chronic adult disease, epi...

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Detalles Bibliográficos
Clasificación:Libro Electrónico
Otros Autores: Croy, B. A. (B. Anne) (Editor ), Yamada, Aureo T. (Editor ), DeMayo, Francesco J. (Editor ), Adamson, S. Lee, 1954- (Editor )
Formato: Electrónico eBook
Idioma:Inglés
Publicado: Amsterdam : Elsevier/Academic Press, [2014]
Temas:
Acceso en línea:Texto completo
Tabla de Contenidos:
  • Part I: Atlas. The cycling and pregnant mouse: gross anatomy
  • Implant site dissections
  • Microscopic anatomy of the pregnant mouse uterus throughout gestation
  • Anatomy of the mouse placenta throughout gestation
  • Whole-mount immunohistochemistry of early implantation sites
  • Magnetic resonance imaging of the mouse placenta
  • Part II: Basic biology. Reproductive tract changes during the mouse estrous cycle
  • First cell fate decisions in early development: towards establishment of the trophoblast lineage
  • Tetraploid complementation assay
  • Biology of the ectoplacental cone
  • Decidualization and endometrial extracellular matrix remodeling
  • Postimplantation development of the chorioallantoic placenta
  • Unique features of endometrial dynamics during pregnancy
  • Pathology analysis of the placenta
  • Changes in structures and relationships of trophoblasts, leukocytes, and maternal vessels in implantation sites to midpregnancy
  • The uteroplacental, fetoplacental, and yolk sac circulations in the mouse
  • Physiological exchange across mouse placenta
  • Hypoxia-associated regulation within implantation sites
  • Leukocyte population dynamics and functions at the maternal--fetal interface
  • In vivo tracking of mononuclear cells in the virgin uterus and in implantation sites
  • Microchimerism in mouse pregnancy
  • Systems biology analyses of the placenta
  • Contribution of placental genomic imprinting and identification of imprinted genes
  • Epigenetics of placental development and function
  • Genome-wide analysis of the mouse deciduoma
  • Conditional gene deletion in the placenta using the Cre-loxP system
  • Genetic engineering of mice to investigate uterine function in early pregnancy
  • Lentivirus-mediated transduction of optical reporter genes in blastocysts for placental studies
  • Blood pressure regulation during mouse pregnancy
  • Intravital imaging of vasoactivity in the uterine arterial tree during pregnancy
  • Multimodal imaging of the mouse placenta
  • Regulation of parturition
  • Cervical changes accompanying birth
  • Peri-partum changes to mouse pubic symphysis
  • Mammary gland morphogenesis and function during pregnancy
  • Gestational programming of the cardiovascular system
  • Maternal stress-mediated gene expression changes in the mouse placenta: a mosaic hypothesis of epigenesis
  • Pregnancy alterations from environmental pollutants
  • Part III: Protocols. Monitoring mouse estrous cycles
  • Lentivirus transduction of gene tags in blastocysts
  • Tetraploid complementation assay
  • Pseudopregnant bead-induced mouse deciduoma model
  • Ectoplacental cone isolation, culture and assessment
  • Layer-enriched tissue dissection of the mouse placenta in late gestation
  • Protocols for placental histology
  • Best practice for quantifying the microscopic structure of mouse placenta: the stereological approach
  • Electron microscopy and immunoelectromicroscopy protocols
  • Laser capture microdissection
  • Immunofluorescence and immunohistochemistry
  • Nonradioactive in situ hybridization: optimization for tissue sections from pregnant uteri and placenta during the first half of pregnancy
  • Whole mount in situ immunohistochemistry
  • Guidelines for evaluation of reproductive and developmental endpoints
  • Flow cytometric analysis of myometrial and decidual cell suspensions
  • Uterine leukocyte isolation and culture
  • Scanning electron microscopy and micro-computed tomography imaging of the utero- and fetoplacental circulations
  • Radiotelemetry in mouse pregnancy
  • Ex vivo perfusion of the mouse placenta for maternal--fetal interaction studies during pregnancy
  • Evaluation of placental transport function
  • Hypoxia-associated regulation within implantation sites
  • Method to locate the uterine artery in mice for micro-ultrasound doppler blood velocity examination
  • Two-photon intravital microscopy of the virgin and pregnant mouse uterus
  • Intravital imaging of vasoactivity in the uterine arterial vasculature tree during pregnancy
  • MRI, intra-vital, and ex-vivo fluorescence microscopy of the mouse uterine vasculature and placenta
  • Assessment of changes in the peripartum cervix
  • Pubic symphysis evaluation
  • Labor and preterm labor
  • Epithelial transplantation into the cleared mammary fat pad
  • Dietary and hypoxic protocols that alter placental gene expression in response to maternal stress
  • Parental-specific gene expression and epigenetic analyses of imprinted genes in mouse placenta
  • A method for obtaining epigenomic data
  • Methods for genetic engineering in mice
  • Appendix: reproductive parameters of common, commercially available mouse strains.