Laboratory methods in enzymology. Volume 530, RNA /

Methods in Enzymology volumes provide an indispensable tool for the researcher. Each volume is carefully written and edited by experts to contain state-of-the-art reviews and step-by-step protocols. In this volume, we have brought together a number of core protocols concentrating on RNA, complementi...

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Detalles Bibliográficos
Clasificación:Libro Electrónico
Otros Autores: Lorsch, Jon (Editor )
Formato: Electrónico eBook
Idioma:Inglés
Publicado: Amsterdam ; Boston : Elsevier/Academic Press, 2013.
Edición:First edition.
Colección:Methods in enzymology ; v. 530.
Temas:
Enzymes.
Biochemistry > Technique.
Biotechnology > Methodology.
Molecular biology > Methodology.
Sequence Analysis, RNA > methods
Biotechnology > methods
Biotechnology > istrumentation
Molecular Biology > methods
Molecular Biology > istrumentation
Enzymes
Biochimie > Technique.
Biotechnologie > M�ethodologie.
Biologie mol�eculaire > M�ethodologie.
enzyme.
SCIENCE > Life Sciences > Biochemistry.
Acceso en línea:Texto completo
Texto completo
Tabla de Contenidos:
  • Computational prediction of RNA secondary structure / Walter N. Moss
  • Reverse-transcription PCR (RT-PCR) / Julia Bachman
  • Northern blotting / Shan L. He and Rachel Green
  • Explanatory chapter: nuclease protection assays / Elizabeth Eyler
  • In vitro transcription from plasmid or PCR-amplified DNA / Julie L. Brunelle and Rachel Green
  • Measurement of in vivo RNA synthesis rates / David S. McPheeters and Jo Ann Wise
  • Method for measuring mRNA decay rate in Saccharomyces cerevisiae / Wenqian Hu and Jeff Coller
  • Analysis of polysomes from bacteria / Daoming Qin and Kurt Fredrick
  • Polysome profile analysis : yeast / Martin Posp�isek and Leos Val�asek
  • Polysome analysis of mammalian cells / Shan L. He and Rachel Green
  • Polysome analysis for determining mRNA and ribosome association in Saccharomyces cerevisiae / Wenqian Hu and Jeff Coller
  • Oligo(dT)-primed RT-PCR isolation of polyadenylated RNA degradation intermediates / Shimyn Slomovic and Gadi Schuster
  • Circularized RT-PCR (cRT-PCR) : analysis of the 5' ends, 3' ends, and poly(A) tails of RNA / Shimyn Slomovic and Gadi Schuster
  • RNA radiolabeling / Rishi Porecha and Daniel Herschlag
  • Fluorescently labeling synthetic RNAs / Max Greenfield and Daniel Herschlag
  • Analysis of RNA by analytical polyacrylamide gel electrophoresis / Alexey Petrov, Albet Tsa, and Joseph D. Puglisi
  • RNA purification by preparative polyacrylamide gel electrophoresis / Alexey Petrov, Tinghe Wu, Elisabetta Viani Puglisi, and Joseph D. Puglisi
  • Explanatory chapter : nucleic acid concentration determination / Sarah Kolitz and Jon Lorsch
  • RNA purification : precipitation methods / Sarah E. Walker and Jon Lorsch
  • Reverse transcriptase dideoxy sequencing of RNA / Sarah E. Walker and Jon Lorsch
  • RNA structure experimental analysis : chemical modification / Zhili Xu and Gloria Culver
  • Structural analysis of RNA backbone using in-line probing / Ali Nahvi and Rachel Green.

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