Laboratory methods in enzymology. DNA / Volume 529 :

Methods in Enzymology volumes provide an indispensable tool for the researcher. Each volume is carefully written and edited by experts to contain state-of-the-art reviews and step-by-step protocols. In this volume, we have brought together a number of core protocols concentrating on DNA, complementi...

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Detalles Bibliográficos
Clasificación:Libro Electrónico
Otros Autores: Lorsch, Jon (Editor )
Formato: Electrónico eBook
Idioma:Inglés
Publicado: Amsterdam ; Boston : Elsevier/Academic Press, [2013]
Edición:First edition.
Colección:Methods in enzymology ; v. 529.
Temas:
Enzymology.
DNA.
Biotechnology > Methodology.
Molecular biology > Methodology.
DNA
Biotechnology > methods
Biotechnology > istrumentation
Molecular Biology > methods
Molecular Biology > istrumentation
Enzymologie.
ADN.
Biotechnologie > M�ethodologie.
Biologie mol�eculaire > M�ethodologie.
SCIENCE > Life Sciences > Biochemistry.
Biotechnology > Methodology
Enzymology
Molecular biology > Methodology
Laboratory Manual
Laboratory manuals
Laboratory manuals.
Manuels de laboratoire.
Acceso en línea:Texto completo
Texto completo
Tabla de Contenidos:
  • Explanatory chapter: PCR primer design / Rub�en �Alvarez-Fern�andez
  • Explanatory chapter: How plasmid preparation kits work / Laura Koontz
  • Explanatory chapter: Introducing exogenous DNA into cells / Laura Koontz
  • Agarose gel electrophoresis / Laura Koontz
  • Analysis of DNA by southern blotting / Gary Glenn and Lefkothea-Vasiliki Andreou
  • Purification of DNA oligos by denaturing polyacrylamide gel electrophoresis (PAGE) / Sara Lopez-Gomollon and Francisco Esteban Nicolas
  • Molecular cloning / Juliane C. Lessard
  • Rapid creation of stable mammalian cell lines for regulated expression of proteins using the Gateway� recombination cloning technology and Flp-In T-REx� lines / Jessica Spitzer, Markus Landthaler, and Thomas Tuschl
  • Restrictionless cloning / Mikkel A. Algire
  • Isolation of plasmid DNA from bacteria / Lefkothea-Vasiliki Andreou
  • Preparation of genomic DNA from bacteria / Lefkothea-Vasiliki Andreou
  • Preparation of genomic DNA from Saccharomyces cerevisiae / Jessica S. Dymond
  • Isolation of genomic DNA from mammalian cells / Cheryl M. Koh
  • Sanger dideoxy sequencing of DNA / Sarah E. Walker and Jon Lorsch
  • Preparation of fragment libraries for next-generation sequencing on the applied biosystems SOLiD platform / Srinivasan Yegnasubramanian
  • Explanatory chapter: Next generation sequencing / Srinivasan Yegnasubramanian
  • Generating mammalian stable cell lines by electroporation / Patti A. Longo, Jennifer M. Kavran, Min-Sung Kim, and Daniel J. Leahy
  • Transient mammalian cell transfection with polyethylenimine (PEI) / Patti A. Longo, Jennifer M. Kavran, Min-Sung Kim, and Daniel J. Leahy
  • Site-directed mutagenesis / Julia Bachman
  • PCR-based random mutagenesis / Jessica S. Dymond
  • Megaprimer method for mutagenesis of DNA / Craig W. Vander Kooi
  • Explanatory chapter: Troubleshooting PCR / Kirstie Canene-Adams
  • Explanatory chapter: Quantitative PCR / Jessica S. Dymond
  • General PCR / Kirstie Canene-Adams
  • Colony PCR / Megan Bergkessel and Christine Guthrie
  • Chemical transformation of yeast / Megan Bergkessel and Christine Guthrie
  • Transformation of E. coli via electroporation / Juliane C. Lessard
  • Transformation of chemically competent E. coli / Rachel Green and Elizabeth J. Rogers.

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