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Laboratory methods in enzymology. DNA / Volume 529 :
Methods in Enzymology volumes provide an indispensable tool for the researcher. Each volume is carefully written and edited by experts to contain state-of-the-art reviews and step-by-step protocols. In this volume, we have brought together a number of core protocols concentrating on DNA, complementi...
| Clasificación: | Libro Electrónico |
|---|---|
| Otros Autores: | |
| Formato: | Electrónico eBook |
| Idioma: | Inglés |
| Publicado: |
Amsterdam ; Boston :
Elsevier/Academic Press,
[2013]
|
| Edición: | First edition. |
| Colección: | Methods in enzymology ;
v. 529. |
| Temas: | |
| Acceso en línea: | Texto completo Texto completo |
MARC
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| 245 | 0 | 0 | |a Laboratory methods in enzymology. |n Volume 529 : |b DNA / |c edited by Jon Lorsch. |
| 250 | |a First edition. | ||
| 264 | 1 | |a Amsterdam ; |a Boston : |b Elsevier/Academic Press, |c [2013] | |
| 300 | |a 1 online resource (lvi, 348 pages) : |b illustrations | ||
| 336 | |a text |b txt |2 rdacontent | ||
| 337 | |a computer |b c |2 rdamedia | ||
| 338 | |a online resource |b cr |2 rdacarrier | ||
| 347 | |a data file | ||
| 490 | 1 | |a Methods in enzymology, |x 0076-6879 ; |v volume 529 | |
| 504 | |a Includes bibliographical references and indexes. | ||
| 505 | 0 | 0 | |g Explanatory chapter: |t PCR primer design / |r Rub�en �Alvarez-Fern�andez -- |g Explanatory chapter: |t How plasmid preparation kits work / |r Laura Koontz -- |g Explanatory chapter: |t Introducing exogenous DNA into cells / |r Laura Koontz -- |t Agarose gel electrophoresis / |r Laura Koontz -- |t Analysis of DNA by southern blotting / |r Gary Glenn and Lefkothea-Vasiliki Andreou -- |t Purification of DNA oligos by denaturing polyacrylamide gel electrophoresis (PAGE) / |r Sara Lopez-Gomollon and Francisco Esteban Nicolas -- |t Molecular cloning / |r Juliane C. Lessard -- |t Rapid creation of stable mammalian cell lines for regulated expression of proteins using the Gateway� recombination cloning technology and Flp-In T-REx� lines / |r Jessica Spitzer, Markus Landthaler, and Thomas Tuschl -- |t Restrictionless cloning / |r Mikkel A. Algire -- |t Isolation of plasmid DNA from bacteria / |r Lefkothea-Vasiliki Andreou -- |t Preparation of genomic DNA from bacteria / |r Lefkothea-Vasiliki Andreou -- Preparation of genomic DNA from Saccharomyces cerevisiae / |r Jessica S. Dymond -- |t Isolation of genomic DNA from mammalian cells / |r Cheryl M. Koh -- |t Sanger dideoxy sequencing of DNA / |r Sarah E. Walker and Jon Lorsch -- |t Preparation of fragment libraries for next-generation sequencing on the applied biosystems SOLiD platform / |r Srinivasan Yegnasubramanian -- |g Explanatory chapter: |t Next generation sequencing / |r Srinivasan Yegnasubramanian -- |t Generating mammalian stable cell lines by electroporation / |r Patti A. Longo, Jennifer M. Kavran, Min-Sung Kim, and Daniel J. Leahy -- |t Transient mammalian cell transfection with polyethylenimine (PEI) / |r Patti A. Longo, Jennifer M. Kavran, Min-Sung Kim, and Daniel J. Leahy -- |t Site-directed mutagenesis / |r Julia Bachman -- |t PCR-based random mutagenesis / |r Jessica S. Dymond -- |t Megaprimer method for mutagenesis of DNA / |r Craig W. Vander Kooi -- |g Explanatory chapter: |t Troubleshooting PCR / |r Kirstie Canene-Adams -- |g Explanatory chapter: |t Quantitative PCR / Jessica S. Dymond -- |t General PCR / |r Kirstie Canene-Adams -- |t Colony PCR / |r Megan Bergkessel and Christine Guthrie -- |t Chemical transformation of yeast / |r Megan Bergkessel and Christine Guthrie -- |t Transformation of E. coli via electroporation / |r Juliane C. Lessard -- |t Transformation of chemically competent E. coli / |r Rachel Green and Elizabeth J. Rogers. |
| 588 | 0 | |a Print version record. | |
| 520 | |a Methods in Enzymology volumes provide an indispensable tool for the researcher. Each volume is carefully written and edited by experts to contain state-of-the-art reviews and step-by-step protocols. In this volume, we have brought together a number of core protocols concentrating on DNA, complementing the traditional content that is found in past, present and future Methods in Enzymology volumes. Indispensable tool for the researcher Carefully written and edited by experts to contain step-by-step protocolsIn this volume we have brought together a number of core protocols concentrating on D. | ||
| 650 | 0 | |a Enzymology. | |
| 650 | 0 | |a DNA. | |
| 650 | 0 | |a Biotechnology |x Methodology. | |
| 650 | 0 | |a Molecular biology |x Methodology. | |
| 650 | 2 | |a DNA |0 (DNLM)D004247 | |
| 650 | 2 | |a Biotechnology |x methods |0 (DNLM)D001709Q000379 | |
| 650 | 2 | |a Biotechnology |x istrumentation | |
| 650 | 2 | |a Molecular Biology |x methods |0 (DNLM)D008967Q000379 | |
| 650 | 2 | |a Molecular Biology |x istrumentation | |
| 650 | 6 | |a Enzymologie. |0 (CaQQLa)201-0269407 | |
| 650 | 6 | |a ADN. |0 (CaQQLa)201-0008510 | |
| 650 | 6 | |a Biotechnologie |0 (CaQQLa)201-0004007 |x M�ethodologie. |0 (CaQQLa)201-0379663 | |
| 650 | 6 | |a Biologie mol�eculaire |0 (CaQQLa)201-0064024 |x M�ethodologie. |0 (CaQQLa)201-0379663 | |
| 650 | 7 | |a SCIENCE |x Life Sciences |x Biochemistry. |2 bisacsh | |
| 650 | 7 | |a Biotechnology |x Methodology |2 fast |0 (OCoLC)fst00832759 | |
| 650 | 7 | |a DNA |2 fast |0 (OCoLC)fst00886555 | |
| 650 | 7 | |a Enzymology |2 fast |0 (OCoLC)fst00913635 | |
| 650 | 7 | |a Molecular biology |x Methodology |2 fast |0 (OCoLC)fst01024745 | |
| 655 | 2 | |a Laboratory Manual |0 (DNLM)D020484 | |
| 655 | 7 | |a Laboratory manuals |2 fast |0 (OCoLC)fst01920776 | |
| 655 | 7 | |a Laboratory manuals. |2 lcgft | |
| 655 | 7 | |a Manuels de laboratoire. |2 rvmgf |0 (CaQQLa)RVMGF-000001214 | |
| 700 | 1 | |a Lorsch, Jon, |e editor. | |
| 776 | 0 | 8 | |i Print version: |t Laboratory methods in enzymology. |b First edition. |d Amsterdam ; Boston : Elsevier/Academic Press, [2013] |z 9780124186873 |w (OCoLC)858970409 |
| 830 | 0 | |a Methods in enzymology ; |v v. 529. | |
| 856 | 4 | 0 | |u https://sciencedirect.uam.elogim.com/science/book/9780124186873 |z Texto completo |
| 856 | 4 | 0 | |u https://sciencedirect.uam.elogim.com/science/bookseries/00766879/529 |z Texto completo |