A practical guide to protein and peptide purification for microsequencing /

Why a Second Edition? The Second Edition provides practical answers to the general question, "How can I obtain useful sequence information from my protein or peptide?" rather than the more specific question asked in the first edition, "How can I obtain the N-terminal sequence?" I...

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Detalles Bibliográficos
Clasificación:Libro Electrónico
Otros Autores: Matsudaira, Paul T.
Formato: Electrónico eBook
Idioma:Inglés
Publicado: San Diego : Academic Press, �1993.
Edición:2nd ed.
Temas:
Proteins > Purification.
Peptides > Purification.
Amino acid sequence.
Amino Acid Sequence
Peptides > isolation & purification
Proteins > isolation & purification
S�equence des acides amin�es.
SCIENCE > Life Sciences > Biochemistry.
Amino acid sequence
Peptides > Purification
Proteins > Purification
Aminos�aurensequenz
Methode
Peptide
Proteine
Reinigung
Sequenzanalyse > Chemie
Proteinas.
Prot�eines > Purification.
S�equen�cage des acides amin�es.
Acceso en línea:Texto completo
Tabla de Contenidos:
  • Introduction / Paul Matsudaira
  • Strategies for obtaining partial amino acid sequence data from small quantities (<500 pmol) of pure or partially purified protein / Harry Charbonneau
  • Enzymatic digestion of proteins and HPLC peptide isolation / Kathryn L. Stone and Kenneth R. Willaims
  • Purification of proteins and peptides by SDS-PAGE / Nancy LeGendre [and others]
  • Internal amino acid sequence analysis of proteins after in situ protease digestion on nitrocellulose / Ruedi Aebersold
  • Mass spectrometric strategies for the structural characterization of proteins / Hubert A. Scoble [and others].

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