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A practical guide to protein and peptide purification for microsequencing /
Why a Second Edition? The Second Edition provides practical answers to the general question, "How can I obtain useful sequence information from my protein or peptide?" rather than the more specific question asked in the first edition, "How can I obtain the N-terminal sequence?" I...
| Clasificación: | Libro Electrónico |
|---|---|
| Otros Autores: | |
| Formato: | Electrónico eBook |
| Idioma: | Inglés |
| Publicado: |
San Diego :
Academic Press,
�1993.
|
| Edición: | 2nd ed. |
| Temas: | |
| Acceso en línea: | Texto completo |
MARC
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| 245 | 0 | 2 | |a A practical guide to protein and peptide purification for microsequencing / |c edited by Paul Matsudaira. |
| 250 | |a 2nd ed. | ||
| 260 | |a San Diego : |b Academic Press, |c �1993. | ||
| 300 | |a 1 online resource (xx, 184 pages) : |b illustrations | ||
| 336 | |a text |b txt |2 rdacontent | ||
| 337 | |a computer |b c |2 rdamedia | ||
| 338 | |a online resource |b cr |2 rdacarrier | ||
| 504 | |a Includes bibliographical references (pages 159-184). | ||
| 505 | 0 | |a Introduction / Paul Matsudaira -- Strategies for obtaining partial amino acid sequence data from small quantities (<500 pmol) of pure or partially purified protein / Harry Charbonneau -- Enzymatic digestion of proteins and HPLC peptide isolation / Kathryn L. Stone and Kenneth R. Willaims -- Purification of proteins and peptides by SDS-PAGE / Nancy LeGendre [and others] -- Internal amino acid sequence analysis of proteins after in situ protease digestion on nitrocellulose / Ruedi Aebersold -- Mass spectrometric strategies for the structural characterization of proteins / Hubert A. Scoble [and others]. | |
| 588 | 0 | |a Print version record. | |
| 520 | |a Why a Second Edition? The Second Edition provides practical answers to the general question, "How can I obtain useful sequence information from my protein or peptide?" rather than the more specific question asked in the first edition, "How can I obtain the N-terminal sequence?" Important new methods include ways of dealing with blocked N termini, computer analysis of protein sequences, and the recent revolution in mass spectrometry. Key Features Highlights of the Second Edition:. Mass spectrophotometric characterization of proteins and peptides. N-terminal sequencing of proteins with blocked N termini. Internal amino acid sequence analysis after protease digestion in-gel and on-blot. Improved microscale peptide purification methods. Computer analysis of protein sequences. New protocols tested and refined through everyday use in authors' laboratories. Updated reference chapter covering all aspects of protein microsequencing | ||
| 546 | |a English. | ||
| 650 | 0 | |a Proteins |x Purification. | |
| 650 | 0 | |a Peptides |x Purification. | |
| 650 | 0 | |a Amino acid sequence. | |
| 650 | 2 | |a Amino Acid Sequence |0 (DNLM)D000595 | |
| 650 | 2 | |a Peptides |x isolation & purification |0 (DNLM)D010455Q000302 | |
| 650 | 2 | |a Proteins |x isolation & purification |0 (DNLM)D011506Q000302 | |
| 650 | 6 | |a S�equence des acides amin�es. |0 (CaQQLa)201-0160535 | |
| 650 | 7 | |a SCIENCE |x Life Sciences |x Biochemistry. |2 bisacsh | |
| 650 | 7 | |a Amino acid sequence |2 fast |0 (OCoLC)fst00807613 | |
| 650 | 7 | |a Peptides |x Purification |2 fast |0 (OCoLC)fst01057574 | |
| 650 | 7 | |a Proteins |x Purification |2 fast |0 (OCoLC)fst01079750 | |
| 650 | 7 | |a Aminos�aurensequenz |2 gnd |0 (DE-588)4192666-3 | |
| 650 | 7 | |a Methode |2 gnd |0 (DE-588)4038971-6 | |
| 650 | 7 | |a Peptide |2 gnd |0 (DE-588)4045125-2 | |
| 650 | 7 | |a Proteine |2 gnd |0 (DE-588)4076388-2 | |
| 650 | 7 | |a Reinigung |2 gnd |0 (DE-588)4049250-3 | |
| 650 | 7 | |a Sequenzanalyse |g Chemie |2 gnd |0 (DE-588)4132277-0 | |
| 650 | 7 | |a Proteinas. |2 larpcal | |
| 650 | 7 | |a S�equence des acides amin�es. |2 ram | |
| 650 | 7 | |a Peptides |x Purification. |2 ram | |
| 650 | 7 | |a Prot�eines |x Purification. |2 ram | |
| 650 | 7 | |a S�equen�cage des acides amin�es. |2 ram | |
| 700 | 1 | |a Matsudaira, Paul T. | |
| 776 | 0 | 8 | |i Print version: |t Practical guide to protein and peptide purification for microsequencing. |b 2nd ed. |d San Diego : Academic Press, �1993 |z 0124802826 |w (DLC) 92048384 |w (OCoLC)27187775 |
| 856 | 4 | 0 | |u https://sciencedirect.uam.elogim.com/science/book/9780124802827 |z Texto completo |