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Immunochemistry : labfax /
LABFAX volumes are purpose-designed data reference books for practicing scientists. Each book presents key information for a major subject in one place and so saves hours of searching. The authors and editors of each LABFAX volume have searched the original literature for the accurate data which the...
| Clasificación: | Libro Electrónico |
|---|---|
| Otros Autores: | , |
| Formato: | Electrónico eBook |
| Idioma: | Inglés |
| Publicado: |
Oxford :
BIOS Scientific Publishers,
1994.
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| Colección: | Labfax series.
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| Temas: | |
| Acceso en línea: | Texto completo |
Tabla de Contenidos:
- Front Cover; Immunochemistry Lab Fax; Copyright Page; Table of Contents; Contributors; Abbreviations; CHAPTER 1. PLASMA PROTEINS; Plasma and serum; Anticoagulants; Serum; Plasma/serum proteins; Factors affecting the concentration of plasma proteins; The effect of age; The acute phase response; Plasma proteins in other body fluids; Antibodies recognizing plasma/serum proteins; Absorption of unwanted specificities; Anti-whole sera antibodies; Purified antibodies; Storage of commercial antibodies; References; CHAPTER 2. PROPERTIES OF IMMUNOGLOBULINS; Introduction; Diversity of immunoglobulins
- Human immunoglobulin allotypesImmunoglobulins from other species; Immunoglobulin receptors (Fc receptors); References; CHAPTER 3. ANTIBODIES RECOGNIZING IMMUNOGLOBULINS; The specificity of antibodies recognizing immunoglobulins of other species; References; CHAPTER 4. PRODUCING ANTIBODIES; Polyclonal antisera; Monoclonal antibodies; rDNA-produced antibodies; References; CHAPTER 5. PURIFICATION AND FRAGMENTATION OF IMMUNOGLOBULINS; General properties of antibodies; Sources of immunoglobulins; Methods of purification of IgG; Purification of IgM; Purification of serum IgA
- Other techniques for immunoglobulin or antibody purificationProperties of bacterial IgG-binding proteins: protein A and protein G; Properties of IgA-binding proteins; Purified immunoglobulins available commercially; Preparation of immunoglobulin fragments; References; CHAPTER 6. PREPARATION AND USE OF RADIOLABELED ANTIBODIES AND ANTIGENS; Labeling with radioisotopes; Labeling with other isotopes; Radioimmunoassay; Immunoradiometric assay; References; CHAPTER 7. ENZYME-CONJUGATED ANTIBODIES: PREPARATION AND USE; Introduction; Enhanced enzyme immunodetection systems
- Enzymes commonly used in immunoconjugatesMethods for the conjugation of enzymes to antibodies; Properties of enzyme substrates commonly used in immunoassay; References; CHAPTER 8. DERIVATIZATION OF ANTIBODIES AND ANTIGENS: FLUORESCENT LABELING, BIOTINYLATION, IMMUNOGOLD DERIVATIVES; Fluorescently labeled antibodies; Biotinylation of proteins; Colloidal gold derivatives; References; CHAPTER 9. COMMON IMMUNOLOGICAL TECHNIQUES: ELISA, BLOTTING, IMMUNOHISTOCHEMISTRY AND IMMUNOCYTOCHEMISTRY; ELISA; Immunoblotting; Immunohistochemistry and immunocytochemistry; References; CHAPTER 10. COMPLEMENT
- IntroductionNomenclature; General properties of complement; Proteins of the complement system; Control of the complement system; The fragmentation of C3; Complement receptors; Detection and estimation of complement components; Antigenic assays for complement components; Functional assays and enzyme assays; Detection of activation products; Biological assays; Complement measurement in disease; References; CHAPTER 11. SAFETY; Chemical safety; Radiochemical safety; Microbiological safety; Viral safety; References; INDEX