Three-Dimensional Confocal Microscopy : Volume Investigation of Biological Specimens /

The integration of confocal microscopy and volume investigation has led to an unprecedented ability to examine spatial relationships between cellular structure and function. The goal of this book is to familiarize the reader with these new technologies and to demonstrate their applicability to a wid...

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Detalles Bibliográficos
Clasificación:Libro Electrónico
Otros Autores: Stevens, John K., Mills, Linda R., Trogadis, Judy E.
Formato: Electrónico eBook
Idioma:Inglés
Publicado: Burlington : Elsevier Science, 1994.
Colección:Cell biology.
Temas:
Confocal microscopy.
Three-dimensional imaging in biology.
Microscopie confocale.
Imagerie tridimensionnelle en biologie.
NATURE > Animals > Wildlife.
SCIENCE > Microscopes & Microscopy.
Confocal microscopy
Three-dimensional imaging in biology
Acceso en línea:Texto completo
Tabla de Contenidos:
  • Front Cover; Three-Dimensional Confocal Microscopy: Volume Investigation of Biological Specimens; Copyright Page; Table of Contents; Preface; PART I: Confocal Microscopy: Practical Considerations; Chapter 1. Introduction to Confocal Three-Dimensional Volume Investigation; I. INTRODUCTION TO VOLUME INVESTIGATION; II. DATA COLLECTION: THE IMAGE STACK; III. SEGMENTING THE IMAGE STACK; IV. THREE-DIMENSIONAL VISUALIZATION; V. THREE-DIMENSIONAL ANALYSIS; VI. VALUE OF VOLUME INVESTIGATION; REFERENCES; Chapter 2. Background Rejection and Optimization of Signal to Noise in Confocal Microscopy.
  • I. INTRODUCTIONII. CALCULATIONS; III. SIGNAL; IV. BACKGROUND; VI. SIGNAL-TO-NOISE RATIO; VII. DISCUSSION; ACKNOWLEDGMENTS; REFERENCES; Chapter 3. Sources of Noise in Three-Dimensional Microscopical Data Sets; I. INTRODUCTION; II. NOTIONAL SPECIMENS; III. THREE-DIMENSIONAL FLUORESCENCE MICROSCOPY; IV. SIGNAL AND CONTRAST IN THREE-DIMENSIONAL MICROSCOPY; V. NOISE SOURCES IN THREE-DIMENSIONAL MICROSCOPY; VI. NOISE IN CONFOCAL MICROSCOPY; VII. NOISE IN WIDE-FIELD METHOD; VIII. CONCLUSIONS; REFERENCES; Chapter 4. Simultaneous Ultraviolet and Visible Wavelength Confocal Microscopy; I. INTRODUCTION.
  • II. DESIGN AND PRINCIPLEIII. SUMMARY; ACKNOWLEDGMENTS; REFERENCES; Chapter 5. Fluorescent Labels for Confocal Microscopy; I. INTRODUCTION; II. CRITERIA FOR FLUORESCENT DYE SELECTION; III. EXCITATION; IV. PHOTOBLEACHING; V. SIGNAL DETECTION AND ISOLATION; VI. INTERMOLECULAR PERTURBATIONS: ENVIRONMENTAL EFFECTS; VII. CONCLUSIONS; ACKNOWLEDGMENTS; REFERENCES; Chapter 6. Display Methods for Gray-Scale, Voxel-Based Data Sets; I. INDIRECT METHODS; II. DIRECT METHODS FOR DISPLAY OF SURFACES IN BINARY DATA; III. DIRECT BINARY METHODS FOR DISPLAY OF SURFACES IN GRAY-SCALE DATA.
  • IV� DIRECT BINARY METHODS FOR DISPLAY OF INTENSITY DISTRIBUTIONS IN GRAY-SCALE DATAV. DIRECT NONBINARY METHODS FOR DISPLAY OF GRAY-SCALE DATA; VI. OBJECT DEFINITION AND SEGMENTATION; VII. CONCLUSIONS; ACKNOWLEDGMENTS; REFERENCES; Chapter 7. Three-Dimensional Volume Reconstruction in Confocal Microscopy: Practical Considerations; I. INTRODUCTION; II. THEORY OF CONFOCAL MICROSCOPY; III. SPECIMEN PREPARATION; IV. OPTIMIZATION OF FIXATION PROTOCOLS; V. VOLUME RECONSTRUCTIONS; VI. EXAMPLES OF THREE-DIMENSIONAL RECONSTRUCTION; VII. CONCLUSION; ACKNOWLEDGMENTS; REFERENCES.
  • PART II: Biological Applications of Confocal MicroscopyChapter 8. Imaging Ion Channels in Live Central Neurons Using Fluorescent Ligands Ligand Construction; I. INTRODUCTION; II. DESIGN OF PROBES; III. INTRODUCTION OF LABELS; IV. PURIFICATION OF LABELS; V. SOLVENT REMOVAL AND STORAGE OF PURIFIED CONJUGATES; VI. CHARACTERIZATION OF PROBES; ACKNOWLEDGMENTS; REFERENCES; Chapter 9. Imaging Ion Channels in Live Central Neurons Using Fluorescent Ligands Labeling of Cells and Tissues; I. LABELING OF CELLS AND TISSUES; II. PROBLEMS; III. LIMITATIONS OF LABELING ION CHANNELS WITH FLUORESCENT LIGANDS.

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