Biochemistry and cell culture /

Cell biology spans among the widest diversity of methods in the biological sciences. From physical chemistry to microscopy, cells have given up with secrets only when the questions are asked in the right way! This new volume of Methods in Cell Biology covers laboratory methods in cell biology, and i...

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Detalles Bibliográficos
Clasificación:Libro Electrónico
Autor principal: Conn, P. Michael
Formato: Electrónico eBook
Idioma:Inglés
Publicado: Amsterdam ; Boston : Elsevier/Academic Press, 2012.
Edición:1st ed.
Colección:Laboratory methods in cell biology, v. 112
Temas:
Cell culture.
Cytology > Technique.
Cellules > Culture.
Cytologie > Technique.
SCIENCE > Life Sciences > Cell Biology.
Acceso en línea:Texto completo
Texto completo
Tabla de Contenidos:
  • Front Cover; Series Editors; Laboratory Methods in Cell Biology: Biochemistry and Cell Culture; Copyright; Contents; Contributors; Preface: Laboratory Methods in CellBiology; CHAPTER1
  • Extraction/Characterization of DNA; 1 PURPOSE; 2 THEORY; 3 EQUIPMENT (IN ORDER OF APPEARANCE); 4 MATERIALS (IN ORDER OF APPEARANCE); 5 PROTOCOL; CHAPTER2
  • CO-IMMUNOPRECIPITATION: ISOLATION OF PROTEIN SIGNALING COMPLEXES FROM NATIVE TISSUES; 1 PURPOSE; 2 THEORY; 3 EQUIPMENT; 4 MATERIALS; 5 PROTOCOL; References; CHAPTER 3
  • REAL-TIME PCR; 1 PURPOSE; 2 THEORY; 3 EQUIPMENTS; 4 MATERIALS; 5 PREPARE WORKSPACE
  • 6 PROTOCOLReferences; CHAPTER4
  • SINGLE-CELL GEL ELECTROPHORESIS COMBINED WITH LESION-SPECIFIC ENZYMES TO MEASURE OXIDATIVE DAMAGE TO DNA; 1 PURPOSE; 2 THEORY; 3 EQUIPMENTS; 4 MATERIALS; 5 PROTOCOL; References; CHAPTER5
  • HIGH-THROUGHPUT METHODS FOR THE IDENTIFICATION OF PROTEIN PURIFICATION CONDITIONS USING A CLEAVABLE TAG SYSTEM; 1 PURPOSE; 2 THEORY; 3 EQUIPMENT; 4 MATERIALS; 5 PROTOCOL; References; CHAPTER6
  • TWO-DIMENSIONAL DIFFERENCE GEL ELECTROPHORESIS (2D DIGE); 1 PURPOSE; 2 THEORY; 3 EQUIPMENT; 4 MATERIALS; 5 PROTOCOL; References
  • CHAPTER7
  • OPTIMIZATION OF CELL CYCLE MEASUREMENT BY TIME-LAPSE MICROSCOPY1 PURPOSE; 2 THEORY; 3 DETERMINATION OF CELL CYCLE LENGTH; 4 EQUIPMENTS; 5 MATERIALS; 6 PROTOCOL; Video; References; CHAPTER8
  • TRANSFECTION METHODS; 1 PURPOSE; 2 THEORY; 3 EQUIPMENT; 4 MATERIALS; 5 PROTOCOL; References; CHAPTER 9
  • CELL ENRICHMENT FROM HUMAN BLOOD THROUGH RED CELL LYSIS; 1 PURPOSE; 2 THEORY; 3 EQUIPMENTS; 4 MATERIALS; 5 PROTOCOL; References; CHAPTER10
  • MAGNETIC, BATCH SEPARATION; 1 PURPOSE; 2 THEORY; 3 EQUIPMENT; 4 MATERIALS; 5 PROTOCOL; References
  • CHAPTER11
  • LATERAL FLOW SANDWICH ASSAY UTILIZING UPCONVERTING PHOSPHOR (UCP) REPORTERS1 PURPOSE; 2 THEORY; 3 EQUIPMENTS; 4 MATERIALS; 5 PROTOCOL; References; CHAPTER 12
  • IN VIVO MODULATION OF MICRORNA LEVELS DURING SPINAL CORD REGENERATION; 1 PURPOSE; 2 THEORY; 3 EQUIPMENTS; 4 MATERIALS; 5 PROTOCOL; References; CHAPTER 13
  • COMETCHIP: SINGLE-CELL MICROARRAY FOR HIGH-THROUGHPUT DETECTION OF DNA DAMAGE; 1 PURPOSE; 2 THEORY; 3 EQUIPMENTS; 4 MATERIALS; 5 PROTOCOL; References; CHAPTER 14
  • PURIFICATION OF CARDIAC CELLS; 1 THEORY; 2 EQUIPMENTS; 3 MATERIALS; 4 PROTOCOL; Reference
  • CHAPTER 15
  • TUBE FORMATION IN VITRO ANGIOGENESIS ASSAY1 PURPOSE; 2 THEORY; 3 EQUIPMENTS; 4 MATERIALS; 5 PROTOCOL; References; CHAPTER 16
  • PROCESSING OF TRANSMISSION ELECTRON MICROSCOPY IMAGES FOR SINGLE-PARTICLE ANALYSIS OF MACROMOLECULAR COMPLEXES; 1 PURPOSE; 2 THEORY; 3 EQUIPMENT; 4 MATERIALS; 5 PROTOCOL; References; CHAPTER 17
  • AN ASYMMETRIC 3D IN VITRO ASSAY FOR THE STUDY OF TUMOR CELL INVASION; 1 PURPOSE; 2 THEORY; 3 EQUIPMENT; 4 MATERIALS; 5 PROTOCOL; References; CHAPTER 18
  • ISOLATION OF MYOBLASTS FROM MUSCLE TISSUE BY PREPLATING; 1 PURPOSE; 2 THEORY; 3 EQUIPMENT; 4 MATERIALS

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