Biochemistry and cell culture /

Cell biology spans among the widest diversity of methods in the biological sciences. From physical chemistry to microscopy, cells have given up with secrets only when the questions are asked in the right way! This new volume of Methods in Cell Biology covers laboratory methods in cell biology, and i...

Descripción completa

Detalles Bibliográficos
Clasificación:Libro Electrónico
Autor principal: Conn, P. Michael
Formato: Electrónico eBook
Idioma:Inglés
Publicado: Amsterdam ; Boston : Elsevier/Academic Press, 2012.
Edición:1st ed.
Colección:Laboratory methods in cell biology, v. 112
Temas:
Cell culture.
Cytology > Technique.
Cellules > Culture.
Cytologie > Technique.
SCIENCE > Life Sciences > Cell Biology.
Acceso en línea:Texto completo
Texto completo

MARC

LEADER 00000cam a2200000Ma 4500
001 SCIDIR_ocn823738967
003 OCoLC
005 20231117044822.0
006 m o d
007 cr cn|||||||||
008 121206s2012 ne af ob 001 0 eng d
040 |a E7B  |b eng  |e pn  |c E7B  |d OCLCQ  |d OCLCO  |d YDXCP  |d N$T  |d OPELS  |d OCLCF  |d OCLCQ  |d OCLCA  |d D6H  |d ESU  |d OCLCQ  |d LEAUB  |d UKBTH  |d S2H  |d OCLCO  |d OCLCQ 
019 |a 1113013498 
020 |a 9780124055490  |q (electronic bk.) 
020 |a 0124055494  |q (electronic bk.) 
020 |z 9780124059146 
020 |a 1283836963 
020 |a 9781283836968 
035 |a (OCoLC)823738967  |z (OCoLC)1113013498 
050 4 |a QH585.2  |b .C66 2012eb 
072 7 |a SCI  |x 017000  |2 bisacsh 
082 0 4 |a 571.6/38  |2 23 
100 1 |a Conn, P. Michael. 
245 1 0 |a Biochemistry and cell culture /  |c P. Michael Conn. 
250 |a 1st ed. 
260 |a Amsterdam ;  |a Boston :  |b Elsevier/Academic Press,  |c 2012. 
300 |a 1 online resource (xix, 474 pages, 10 unnumbered pages of plates) :  |b illustrations. 
336 |a text  |b txt  |2 rdacontent 
337 |a computer  |b c  |2 rdamedia 
338 |a online resource  |b cr  |2 rdacarrier 
490 0 |a Laboratory methods in cell biology,  |x 0091-679X ;  |v v. 112 
504 |a Includes bibliographical references and index. 
520 |a Cell biology spans among the widest diversity of methods in the biological sciences. From physical chemistry to microscopy, cells have given up with secrets only when the questions are asked in the right way! This new volume of Methods in Cell Biology covers laboratory methods in cell biology, and includes methods that are among the most important and elucidating in the discipline, such as transfection, cell enrichment and magnetic batch separation. Covers the most important laboratory methods in cell biology Chapters written by experts in their fields. 
505 0 |a Front Cover; Series Editors; Laboratory Methods in Cell Biology: Biochemistry and Cell Culture; Copyright; Contents; Contributors; Preface: Laboratory Methods in CellBiology; CHAPTER1 -- Extraction/Characterization of DNA; 1 PURPOSE; 2 THEORY; 3 EQUIPMENT (IN ORDER OF APPEARANCE); 4 MATERIALS (IN ORDER OF APPEARANCE); 5 PROTOCOL; CHAPTER2 -- CO-IMMUNOPRECIPITATION: ISOLATION OF PROTEIN SIGNALING COMPLEXES FROM NATIVE TISSUES; 1 PURPOSE; 2 THEORY; 3 EQUIPMENT; 4 MATERIALS; 5 PROTOCOL; References; CHAPTER 3 -- REAL-TIME PCR; 1 PURPOSE; 2 THEORY; 3 EQUIPMENTS; 4 MATERIALS; 5 PREPARE WORKSPACE 
505 8 |a 6 PROTOCOLReferences; CHAPTER4 -- SINGLE-CELL GEL ELECTROPHORESIS COMBINED WITH LESION-SPECIFIC ENZYMES TO MEASURE OXIDATIVE DAMAGE TO DNA; 1 PURPOSE; 2 THEORY; 3 EQUIPMENTS; 4 MATERIALS; 5 PROTOCOL; References; CHAPTER5 -- HIGH-THROUGHPUT METHODS FOR THE IDENTIFICATION OF PROTEIN PURIFICATION CONDITIONS USING A CLEAVABLE TAG SYSTEM; 1 PURPOSE; 2 THEORY; 3 EQUIPMENT; 4 MATERIALS; 5 PROTOCOL; References; CHAPTER6 -- TWO-DIMENSIONAL DIFFERENCE GEL ELECTROPHORESIS (2D DIGE); 1 PURPOSE; 2 THEORY; 3 EQUIPMENT; 4 MATERIALS; 5 PROTOCOL; References 
505 8 |a CHAPTER7 -- OPTIMIZATION OF CELL CYCLE MEASUREMENT BY TIME-LAPSE MICROSCOPY1 PURPOSE; 2 THEORY; 3 DETERMINATION OF CELL CYCLE LENGTH; 4 EQUIPMENTS; 5 MATERIALS; 6 PROTOCOL; Video; References; CHAPTER8 -- TRANSFECTION METHODS; 1 PURPOSE; 2 THEORY; 3 EQUIPMENT; 4 MATERIALS; 5 PROTOCOL; References; CHAPTER 9 -- CELL ENRICHMENT FROM HUMAN BLOOD THROUGH RED CELL LYSIS; 1 PURPOSE; 2 THEORY; 3 EQUIPMENTS; 4 MATERIALS; 5 PROTOCOL; References; CHAPTER10 -- MAGNETIC, BATCH SEPARATION; 1 PURPOSE; 2 THEORY; 3 EQUIPMENT; 4 MATERIALS; 5 PROTOCOL; References 
505 8 |a CHAPTER11 -- LATERAL FLOW SANDWICH ASSAY UTILIZING UPCONVERTING PHOSPHOR (UCP) REPORTERS1 PURPOSE; 2 THEORY; 3 EQUIPMENTS; 4 MATERIALS; 5 PROTOCOL; References; CHAPTER 12 -- IN VIVO MODULATION OF MICRORNA LEVELS DURING SPINAL CORD REGENERATION; 1 PURPOSE; 2 THEORY; 3 EQUIPMENTS; 4 MATERIALS; 5 PROTOCOL; References; CHAPTER 13 -- COMETCHIP: SINGLE-CELL MICROARRAY FOR HIGH-THROUGHPUT DETECTION OF DNA DAMAGE; 1 PURPOSE; 2 THEORY; 3 EQUIPMENTS; 4 MATERIALS; 5 PROTOCOL; References; CHAPTER 14 -- PURIFICATION OF CARDIAC CELLS; 1 THEORY; 2 EQUIPMENTS; 3 MATERIALS; 4 PROTOCOL; Reference 
505 8 |a CHAPTER 15 -- TUBE FORMATION IN VITRO ANGIOGENESIS ASSAY1 PURPOSE; 2 THEORY; 3 EQUIPMENTS; 4 MATERIALS; 5 PROTOCOL; References; CHAPTER 16 -- PROCESSING OF TRANSMISSION ELECTRON MICROSCOPY IMAGES FOR SINGLE-PARTICLE ANALYSIS OF MACROMOLECULAR COMPLEXES; 1 PURPOSE; 2 THEORY; 3 EQUIPMENT; 4 MATERIALS; 5 PROTOCOL; References; CHAPTER 17 -- AN ASYMMETRIC 3D IN VITRO ASSAY FOR THE STUDY OF TUMOR CELL INVASION; 1 PURPOSE; 2 THEORY; 3 EQUIPMENT; 4 MATERIALS; 5 PROTOCOL; References; CHAPTER 18 -- ISOLATION OF MYOBLASTS FROM MUSCLE TISSUE BY PREPLATING; 1 PURPOSE; 2 THEORY; 3 EQUIPMENT; 4 MATERIALS 
546 |a English. 
650 0 |a Cell culture. 
650 0 |a Cytology  |x Technique. 
650 6 |a Cellules  |x Culture.  |0 (CaQQLa)201-0005038 
650 6 |a Cytologie  |x Technique.  |0 (CaQQLa)201-0005040 
650 7 |a SCIENCE  |x Life Sciences  |x Cell Biology.  |2 bisacsh 
650 7 |a Cell culture.  |2 fast  |0 (OCoLC)fst00850172 
650 7 |a Cytology  |x Technique.  |2 fast  |0 (OCoLC)fst00886306 
856 4 0 |u https://sciencedirect.uam.elogim.com/science/book/9780124059146  |z Texto completo 
856 4 0 |u https://sciencedirect.uam.elogim.com/science/bookseries/0091679X/112  |z Texto completo 

Ejemplares similares