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Guide to electroporation and electrofusion /
Electroporation is an efficient method to introduce macromolecules such as DNA into a wide variety of cells. Electrofusion results in the fusion of cells and can be used to produce genetic hybrids or hybridoma cells. Guide to Electroporation and Electrofusion is designed to serve the needs of studen...
| Clasificación: | Libro Electrónico |
|---|---|
| Otros Autores: | |
| Formato: | Electrónico eBook |
| Idioma: | Inglés |
| Publicado: |
San Diego :
Academic Press,
�1992.
|
| Temas: | |
| Acceso en línea: | Texto completo Texto completo |
MARC
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| 042 | |a dlr | ||
| 050 | 4 | |a QH585.5.E48 |b G85 1992eb | |
| 060 | 4 | |a QH 585.5.E48 |b G946 1992 | |
| 070 | |a QH585.5.E48G85 |b 1992 | ||
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| 072 | 7 | |a SCI |x 008000 |2 bisacsh | |
| 082 | 0 | 4 | |a 574.8 |2 22 |
| 084 | |a 42.15 |2 bcl | ||
| 245 | 0 | 0 | |a Guide to electroporation and electrofusion / |c Donald C. Chang [and others]. |
| 260 | |a San Diego : |b Academic Press, |c �1992. | ||
| 300 | |a 1 online resource (x, 581 pages) : |b illustrations | ||
| 336 | |a text |b txt |2 rdacontent | ||
| 337 | |a computer |b c |2 rdamedia | ||
| 338 | |a online resource |b cr |2 rdacarrier | ||
| 504 | |a Includes bibliographical references and index. | ||
| 520 | |a Electroporation is an efficient method to introduce macromolecules such as DNA into a wide variety of cells. Electrofusion results in the fusion of cells and can be used to produce genetic hybrids or hybridoma cells. Guide to Electroporation and Electrofusion is designed to serve the needs of students, experienced researchers, and newcomers to the field. It is a comprehensive manual that presents, in one source, up-to-date, easy-to-follow protocols necessary for efficient electroporation and electrofusion of bacteria, yeast, and plant and animal cells, as well as background information to help users optimize their results through comprehension of the principles behind these techniques. Key Features. Covers fundamentals of electroporation and electrofusion in detail. Molecular events. Mechanisms. Kinetics. Gives extensive practical information. The latest applications. Controlling parameters to maximize efficiency. Available instrumentation. Presents applications of electroporation and electrofusion in current research situations. State-of-the-art modifications to electrical pulses and generators. Application of electroporation and electrofusion to unique, alternative cell and tissue types. Gives straightforward, detailed, easy-to-follow protocols for. Formation of human hybridomas. Introduction of genetic material into plant cells and pollen. Transfection of mammalian cells. Transformation of bacteria, plants, and yeast. Production of altered embryos. Optimization of electroporation by using reporter genes. Comprehensive and up-to-date. Convenient bench-top format. Approximately 125 illustrations complement the text. Complete references with article titles. Written by leading authorities in electroporation and electrofusion. | ||
| 538 | |a Master and use copy. Digital master created according to Benchmark for Faithful Digital Reproductions of Monographs and Serials, Version 1. Digital Library Federation, December 2002. |u http://purl.oclc.org/DLF/benchrepro0212 |5 MiAaHDL | ||
| 583 | 1 | |a digitized |c 2010 |h HathiTrust Digital Library |l committed to preserve |5 MiAaHDL |2 pda | |
| 588 | 0 | |a Print version record. | |
| 505 | 0 | |a pt. 1. Mechanisms and fundamental processes in electroporation and electrofusion -- pt. 2. Applications of electroporation and electrofusion in current research -- pt. 3. Practical protocols for electroporation and electrofusion -- pt. 4. Instrumentation for electroporation and electrofusion. | |
| 650 | 0 | |a Electroporation. | |
| 650 | 0 | |a Electrofusion. | |
| 650 | 0 | |a Cell hybridization. | |
| 650 | 0 | |a Membrane fusion. | |
| 650 | 2 | |a Cell Fusion |0 (DNLM)D002459 | |
| 650 | 2 | |a Electricity |0 (DNLM)D004560 | |
| 650 | 2 | |a Membrane Fusion |0 (DNLM)D008561 | |
| 650 | 6 | |a �Electroporation. |0 (CaQQLa)201-0193278 | |
| 650 | 6 | |a �Electrofusion. |0 (CaQQLa)201-0193276 | |
| 650 | 6 | |a Cellules |x Hybridation. |0 (CaQQLa)201-0005044 | |
| 650 | 6 | |a �Electricit�e. |0 (CaQQLa)201-0005619 | |
| 650 | 6 | |a Fusion membranaire. |0 (CaQQLa)201-0026139 | |
| 650 | 7 | |a electricity. |2 aat |0 (CStmoGRI)aat300055232 | |
| 650 | 7 | |a SCIENCE |x Life Sciences |x Biology. |2 bisacsh | |
| 650 | 7 | |a Membrane fusion. |2 fast |0 (OCoLC)fst01015841 | |
| 650 | 7 | |a Cell hybridization. |2 fast |0 (OCoLC)fst00850188 | |
| 650 | 7 | |a Electrofusion. |2 fast |0 (OCoLC)fst00906457 | |
| 650 | 7 | |a Electroporation. |2 fast |0 (OCoLC)fst00907734 | |
| 650 | 7 | |a Elektroporation |2 gnd |0 (DE-588)4237755-9 | |
| 650 | 7 | |a Aufsatzsammlung |2 gnd |0 (DE-588)4143413-4 | |
| 650 | 7 | |a Elektrofusion |2 gnd |0 (DE-588)4263948-7 | |
| 650 | 7 | |a Bio�electrochimie. |2 ram | |
| 650 | 7 | |a Cytologie |x Technique. |2 ram | |
| 650 | 7 | |a �Electroporation. |2 ram | |
| 650 | 7 | |a �Electrofusion. |2 ram | |
| 653 | 0 | |a Cytology | |
| 700 | 1 | |a Chang, Donald C. | |
| 776 | 0 | 8 | |i Print version: |t Guide to electroporation and electrofusion. |d San Diego : Academic Press, �1992 |z 1299193528 |w (DLC) 91018976 |w (OCoLC)23868123 |
| 856 | 4 | 0 | |u https://sciencedirect.uam.elogim.com/science/book/9780080917276 |z Texto completo |
| 856 | 4 | 0 | |u https://sciencedirect.uam.elogim.com/science/book/9780121680404 |z Texto completo |