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Transgenic animal technology : a laboratory handbook /
Transgenic animal technologies and the ability to introduce functional genes into animals have revolutionized our ability to address complex biomedical and biological questions. This well-illustrated handbook covers the technical aspects of gene transfer - from molecular methods to whole animal cons...
| Clasificación: | Libro Electrónico |
|---|---|
| Otros Autores: | |
| Formato: | Electrónico eBook |
| Idioma: | Inglés |
| Publicado: |
Amsterdam ; Boston :
Academic Press,
�2002.
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| Edición: | 2nd ed. |
| Temas: | |
| Acceso en línea: | Texto completo |
Tabla de Contenidos:
- Front Cover; Transgenic Animal Technology: A Laboratory Handbook; Copyright Page; Table of Contents; Contributors; Preface; PART 1: Overview; Chapter 1. Introduction to Transgenic Animal Technology; I. Introduction; II. Historical Background; III. Applications and Overview of Text; References; PART 2: Transgenic Animal Production Focusing on the Mouse Model; Chapter 2. DNA Microinjection and Transgenic Animal Production; I. Introduction; II. General Methods; III. Summary; Acknowledgments; References; Appendix; Chapter 3. Factors Affecting Transgenic Animal Production; I. Introduction.
- II. Getting StartedIII. Troubleshooting; IV. Transgenic Phenomenology; V. Summary; Acknowledgments; References; Chapter 4. Gene Targeting in Embryonic Stem Cells:; I. Stem Cells of the Embryo; II. Gene Targeting in Embryonic Stem Cells; III. Methods; Acknowledgments; References; Chapter 5. Gene Targeting in Embryonic Stem Cells:; I. Introduction; II. Conditional Technologies; III. Cre-loxP System Review, Development and Testing; IV. Cre-loxP and Transgenic Models; V. Methods; VI. Summary; Acknowledgments; References; Chapter 6. Retrovirus-Mediated Gene Transfer Teoan Kim.
- I. Introduction and DiscussionII. Methods; Acknowledgment; References; Chapter 7. Nuclear Transfer Technologies Yukio Tsunoda and Yoko Kato; I. Introduction and Discussion; II. Materials and Equipment; III. Preparation of Microtools; IV. Nuclear Transfer of Preimplantation Embryos; V. Nuclear Transfer of Embryonic Stem Cells and Somatic Cells; Acknowledgment; References; PART 3: Production of Transgenic Laboratory and Domestic Animal Species; Chapter 8. Production of Transgenic Rats; I. Introduction; II. Importance of Rats; III. Contributions from Transgenic Rats; IV. Production.
- V. ConclusionsReferences; Chapter 9. Production of Transgenic Rabbits; I. Applications of Transgenic Rabbits; II. Manipulation of Rabbit Embryos for Production of Transgenics; III. Materials and Methods; References; Chapter 10. Production of Transgenic Fish; I. Introduction; II. Discussion; III. Methods of Gene Transfer; IV. Establishing and Maintaining Transgenic Lineages; V. Care and Containment of Transgenic Fish; VI. Future of the Transgenic Fish; Acknowledgments; References; Chapter 11. Production of Transgenic Poultry; I. Introduction and Discussion; II. Methods; III. Summary.