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Immunology of infection /
| Clasificación: | Libro Electrónico |
|---|---|
| Otros Autores: | , |
| Formato: | Electrónico eBook |
| Idioma: | Inglés |
| Publicado: |
San Diego :
Academic Press,
�1998.
|
| Colección: | Methods in microbiology ;
v. 25. |
| Temas: | |
| Acceso en línea: | Texto completo Texto completo |
Tabla de Contenidos:
- Contributors
- Preface
- Introduction: The immune response to infectious agents
- I. General Methods
- 1. Phenotyping and separation of leukocyte populations based on affinity labelling
- 2. Measurement of cellular proliferation
- 3. Prediction and determination of MHC ligands and T-cell epitopes
- II. Murine Models
- 1. Management of immunocompromised and infected animals
- 2. In vitro analysis
- 2.1. Isolation and preparation of lymphocytes from infected animals
- 2.2. Establishment of murine T-cell lines and clones, hybridomas and transfectomas
- 2.3. Killer cell assays
- 2.4. Measuring cytokine responses by ELISA, ELISPOT and RT-PCR methods
- 2.5. Isolation of and measuring the function of professional phagocytes: Murine macrophages
- 3. In vivo analysis
- 3.1. The immune response in mice challenged with bacterial infections
- 3.2. Measuring immune responses
- 4. Specific models
- 4.1. Murine models of tuberculosis
- 4.2. The leishmaniasis model
- 5. Art and science of DNA vaccines
- 6. Preparation and use of adjuvants
- III. Human System
- 1. Isolation and propagation of human dendritic cells
- 2. Isolation of T cells and establishment of T-cell lines and clones
- 3. Growth transformation of human T cells
- 4. Generation and characterization of killer cells
- 5. Measuring human cytokine responses
- 6. Measuring immune responses in situ: Immunohistology and in situ hybridization
- 7. Isolation, characterization and cultivation of human monocytes and macrophages
- Index.