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Mass spectrometry /

This volume presents a compilation of experimental methods and principles, with detailed protocols and examples. Techniques which have demonstrated value in problem-solving are included. Intended for biochemists, cell biologists, microbiologists, pharmacologists, biomedical scientists, biophysicists...

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Detalles Bibliográficos
Clasificación:Libro Electrónico
Otros Autores: McCloskey, J. A. (James A.) (Editor )
Formato: Electrónico eBook
Idioma:Inglés
Publicado: San Diego : Academic Press, �1990.
Colección:Methods in enzymology ; v. 193.
Temas:
Acceso en línea:Texto completo
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Tabla de Contenidos:
  • Methods of ionization / Alex G. Harrison and Robert J. Cotter
  • Mass analyzers / Keith R. Jennings and Gregory G. Dolnikowski
  • Detectors / Syd Evans
  • Selected-ion measurements / J. Throck Watson
  • Liquid chromatography-mass spectrometry / Marvin L. Vestal
  • Tandem mass spectrometry: Multisector magnetic instruments / Michael L. Gross
  • Tandem mass spectrometry: Quadrupole and hybrid instruments / Richard A. Yost and Robert K. Boyd
  • Matrix selection for liquid secondary ion and fast atom bombardment mass spectrometry / Edwin de Pauw
  • Continuous-flow fast atom bombardment mass spectrometry / Richard M. Caprioli and William T. Moore
  • Collision-induced dissociation / Roger N. Hayes and Michael L. Gross
  • Principles of californium-252 plasma desorption mass spectrometry applied to protein analysis / Ronald D. Macfarlane
  • Mass spectrometry of peptides and proteins by matrix-assisted ultraviolet laser desorption/ionization / Franz Hillenkamp and Michael Karas
  • Utility of exact mass measurements / Klaus Biemann
  • Selection and use of mass reference standards / Richard M. Milberg
  • Chemical derivatization for mass spectrometry / Daniel R. Knapp
  • Introduction of deuterium by exchange for measurement by mass spectrometry / James A. McCloskey
  • Preparation of labeled molecules by exchange with oxygen-18 water / Robert C. Murphy and Keith L. Clay
  • Peptides and proteins: Overview and strategy / Klaus Biemann
  • Enzymatic and chemical digestion of proteins for mass spectrometry / Terry D. Lee and John E. Shively
  • Strategies for locating disulfide bonds in proteins / David L. Smith and Zhongrui Zhou
  • Reversed-phase high-performance liquid chromatography for fractionation of enzymatic digests and chemical cleavage products of proteins / Kathryn L. Stone [and others]
  • Electrospray ionization mass spectrometry / Charles G. Edmonds and Richard D. Smith.
  • Sample preparation for plasma desorption mass spectrometry / Peter Roepstorff
  • Molecular weight analysis of proteins / Ian Jardine
  • Sequencing of peptides by tandem mass spectrometry and high-energy collision-induced dissociation / Klaus Biemann
  • Liquid secondary ion mass spectrometry of phosphorylated and sulfated peptides and proteins / Bradford W. Gibson and Philip Cohen
  • Identification of attachment sites and structural classes of asparagine-linked carbohydrates in glycoproteins / Steven A. Carr [and others]
  • Characterization of recombinant proteins / Hubert A. Scoble and Stephen A. Martin
  • Glycoconjugates: Overview and strategy / Roger A. Laine
  • Linkage analysis using lindberg method / Carl G. Hellerqvist
  • Linkage analysis using reductive cleavage method / Gary R. Gray
  • Linkage positions in glycoconjugates by periodate oxidation and fast atom bombardment mass spectrometry / Anne-Sophie Angel and Bo Nilsson
  • Tandem mass spectrometry in structural characterization of oligosaccharide residues in glycoconjugates / Wilhelm J. Richter [and others]
  • Mass spectrometry of mixtures of intact glycosphingolipids / Bo E. Samuelsson [and others]
  • Preparation and desorption mass spectrometry of permethyl and peracetyl derivatives of oligosaccharides / Anne Dell
  • Desorption mass spectrometry of oligosaccharides coupled with hydrophobic chromophores / Linda Poulter and A.L. Burlingame
  • Oligosaccharide characterization with high-energy collision-induced dissociation mass spectrometry / Beth L. Gillece-Castro and A.L. Burlingame
  • Desorption mass spectrometry of glycosphingolipids / Jasna Peter-Katalinic and Heinz Egge
  • High-mass gas chromatography-mass spectrometry of permethylated oligosaccharides / Gunnar C. Hansson and Hasse Karlsson
  • Tandem mass spectrometry of glycolipids / Catherine E. Costello and James E. Vath
  • Constituents of nucleic acids: Overview and strategy / James A. McCloskey
  • Preparation and enzymatic hydrolysis of DNA and RNA for mass spectrometry / Pamela F. Crain
  • Preparation of trimethylsilyl derivatives of nucleic acid components for analysis by mass spectrometry / Karl H. Schram
  • Analysis of RNA hydrolyzates by liquid chromatography-mass spectrometry / Steven C. Pomerantz and James A. McCloskey
  • Electron ionization mass spectra of trimethylsilyl derivatives of nucleosides / James A. McCloskey
  • Gas chromatography--mass spectrometry of free radical-induced products of pyrimidines and purines in DNA / Miral Dizdaroglu
  • Analysis of 5-methylcytosine in DNA by isotope dilution gas chromatography--mass spectrometry / Pamela F. Crain.