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Methods in methane metabolism. Volume 495, Part B, Methanotrophy /
Produced by microbes on a large scale, methane is an important alternative fuel as well as a potent greenhouse gas. This volume focuses on microbial methane metabolism, which is central to the global carbon cycle. Both methanotrophy and methanogenesis are covered in detail. Topics include isolation...
| Clasificación: | Libro Electrónico |
|---|---|
| Otros Autores: | , |
| Formato: | Electrónico eBook |
| Idioma: | Inglés |
| Publicado: |
Amsterdam [Netherlands] ; Boston [Mass.] :
Elsevier/Academic Press,
2011.
|
| Colección: | Methods in enzymology ;
volume 495. |
| Temas: | |
| Acceso en línea: | Texto completo Texto completo |
Tabla de Contenidos:
- 1.Characterization of the Recombinant Pyrophosphate-Dependent 6-Phosphofructokinases from Methylomicrobium alcaliphilum 20Z and Methylococcus capsulatus Bath / Yuri A. Trotsenko
- 1.Introduction
- 2.Methods
- 3.Brief Overview of Current Knowledge of PPi-PFK in Microorganisms
- 4.Properties of PPi-PFK from Methanotrophs
- 5.Conclusion
- Acknowledgments
- References
- 2.Genes and Enzymes of Ectoine Biosynthesis in Halotolerant Methanotrophs / Yuri A. Trotsenko
- 1.Introduction
- 2.PCR-Based Approach for Identification of the Ectoine Biosynthesis Genes in Methanotrophs
- 3.Transcriptional Regulation of the Ectoine Biosynthesis Genes
- 4.Key Enzymes of Ectoine Biosynthesis
- 5.Conclusion
- Acknowledgments
- References
- 3.Facultative and Obligate Methanotrophs: How to Identify and Differentiate Them / Peter F. Dunfield
- 1.Introduction
- 2.Identification of Methanotrophic Capabilities in Novel Isolates
- 3.Substrate Utilization Tests
- 4.Tests for Culture Purity
- References
- 4.Approaches for the Characterization and Description of Novel Methanotrophic Bacteria / John P. Bowman
- 1.Introduction to Aerobic Methanotroph Characterization for Systematic Research and Other Purposes
- 2.Broad Level Systematic Diversity
- 3.Methodological Approaches
- 4.Concluding Remarks
- References
- 5.Methylococcus capsulatus (Bath): From Genome to Protein Function, and Vice Versa / Harald B. Jensen
- 1.Introduction
- 2.Mapping of the Outer Membrane Proteome of M. capsulatus
- 3.Inner Membrane Protein Complexes Studied with Blue-Native Polyacrylamide Gel Electrophoresis (BN-PAGE)
- 4.Visualization and Identification of M. capsulatus c-type Heme Proteins
- Acknowledgments
- References
- 6.Functional Metagenomics of Methylotrophs / Ludmila Chistoserdova
- 1.Introduction
- 2.Enrichment for Specific Functional Types Using SIP
- 3.Creating a Metagenomic Database and Linking It to Functionality
- 4.Ultrashort Read-Based Metatranscriptomics
- 5.Conclusions and Future Perspectives
- Acknowledgments
- References
- 7.Genetic Systems for Moderately Halo(alkali)philic Bacteria of the Genus Methylomicrobium / Marina G. Kalyuzhnaya
- 1.Introduction
- 2.Methods
- 3.Outlook
- Acknowledgments
- References
- 8.Development of a System for Genetic Manipulation of the Facultative Methanotroph Methylocella silvestris BL2 / J. Colin Murrell
- 1.Introduction
- 2.Growth of M. silvestris
- 3.Introduction of Plasmid DNA by Conjugation and Electroporation
- 4.Gene Deletion by Electroporation of Linear DNA
- 5.Preparation of Competent Cells for Electroporation of M. silvestris
- 6.Construction of Linear DNA for Gene Deletion by Homologous Recombination
- 7.Electroporation
- 8.Efficiency of Gene Deletion
- 9.Case Study: Deletion of Isocitrate Lyase
- 10.Complementation
- 11.Phenotype of the Isocitrate Lyase Deletion Mutant
- 12.Conclusions
- References
- 9.Mutagenesis of Soluble Methane Monooxygenase / J. Colin Murrell
- 1.Introduction
- 2.Bacteriological Growth Media and Antibiotics
- 3.Mutagenesis and Subcloning
- 4.Expression Hosts and Conjugation
- 5.Analysis of Mutants
- Acknowledgments
- References
- 10.Single Cell Methods for Methane Oxidation Analysis / Mary E. Lidstrom
- 1.Introduction
- 2.Methods
- Acknowledgment
- References
- 11.Methanotroph Outer Membrane Preparation / Anne Fjellbirkeland
- 1.Introduction
- 2.Isolation of OMs
- 3.Enrichment of Integral and Tightly Associated OMPs
- 4.Extraction of Surface-Associated Proteins
- 5.Biotin Labeling of Surface-Exposed OMPs
- References
- 12.Overexpression and Purification of the Particulate Methane Monooxygenase from Methylococcus capsulatus (Bath) / Steve S.-F. Yu
- 1.Introduction
- 2.Overproduction of pMMO
- 3.Isolation and Purification of pMMO from the pMMO-Enriched Membranes
- 4.Characterization of the Purified pMMO-Detergent Complex
- Acknowledgments
- References
- 13.Metal Reconstitution of Particulate Methane Monooxygenase and Heterologous Expression of the pmoB Subunit / Amy C. Rosenzweig
- 1.Introduction
- 2.Preparation of apo pMMO Membranes and Metal Reconstitution
- 3.Soluble Domain Constructs of the pmoB Subunit
- Acknowledgments
- References
- 14.Particulate Methane Monooxygenase from Methylosinus trichosporium OB3b / Akimitsu Miyaji
- 1.Introduction
- 2.Bacterial Cells Expressing pMMO
- 3.Membrane-Bound Form of pMMO
- 4.Purification of Detergent-Solubilized pMMO
- 5.Summary
- Acknowledgments
- References
- 15.Production, Isolation, Purification, and Functional Characterization of Methanobactins / Hyung J. Kim
- 1.Introduction: Copper, Siderophores, Chalkophores, and Methanobactin
- 2.Methanotroph Growth and Optimizing Methanobactin Production
- 3.Methanobactin Isolation and Purification
- 4.Studying Methanobactins in Pseudonatural Environments
- Acknowledgments
- References
- 16.A Simple Assay for Screening Microorganisms for Chalkophore Production / Jeremy D. Semrau
- 1.Introduction
- 2.Cu-CAS Assay for Chalkophore Detection
- 3.Fe-CAS Assay for Detecting Nonspecific Binding of Copper from Cu-CAS by a Siderophore
- 4.Conclusions
- Acknowledgments
- References
- 17.Isolation of Methanobactin from the Spent Media of Methane-Oxidizing Bacteria / Alan A. DiSpirito
- 1.Introduction
- 2.Isolation of Methanobactin from the Spent Media of MOB
- 3.Purification of mb
- 4.Sample Variability
- Acknowledgments.