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Methods in methane metabolism. Volume 495, Part B, Methanotrophy /
Produced by microbes on a large scale, methane is an important alternative fuel as well as a potent greenhouse gas. This volume focuses on microbial methane metabolism, which is central to the global carbon cycle. Both methanotrophy and methanogenesis are covered in detail. Topics include isolation...
| Clasificación: | Libro Electrónico |
|---|---|
| Otros Autores: | , |
| Formato: | Electrónico eBook |
| Idioma: | Inglés |
| Publicado: |
Amsterdam [Netherlands] ; Boston [Mass.] :
Elsevier/Academic Press,
2011.
|
| Colección: | Methods in enzymology ;
volume 495. |
| Temas: | |
| Acceso en línea: | Texto completo Texto completo |
MARC
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| 003 | OCoLC | ||
| 005 | 20231117044601.0 | ||
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| 008 | 110403s2011 ne af ob 001 0 eng d | ||
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| 019 | |a 704379301 | ||
| 020 | |a 9780123869050 |q (electronic bk.) | ||
| 020 | |a 0123869056 |q (electronic bk.) | ||
| 035 | |a (OCoLC)710966446 |z (OCoLC)704379301 | ||
| 050 | 4 | |a QP601 |b .C733 v.495 | |
| 082 | 0 | 4 | |a 574.1925 |2 22 |
| 245 | 0 | 0 | |a Methods in methane metabolism. |n Volume 495, |p Part B, |p Methanotrophy / |c edited by Amy C. Rosenzweig, Stephen W. Ragsdale. |
| 260 | |a Amsterdam [Netherlands] ; |a Boston [Mass.] : |b Elsevier/Academic Press, |c 2011. | ||
| 300 | |a 1 online resource (li, 591 pages, 16 unnumbered pages of plates) : |b illustrations. | ||
| 336 | |a text |b txt |2 rdacontent | ||
| 337 | |a computer |b c |2 rdamedia | ||
| 338 | |a online resource |b cr |2 rdacarrier | ||
| 347 | |a data file |2 rda | ||
| 490 | 1 | |a Methods in enzymology ; |v v. 495 | |
| 504 | |a Includes bibliographical references and indexes. | ||
| 588 | 0 | |a Print version record. | |
| 520 | |a Produced by microbes on a large scale, methane is an important alternative fuel as well as a potent greenhouse gas. This volume focuses on microbial methane metabolism, which is central to the global carbon cycle. Both methanotrophy and methanogenesis are covered in detail. Topics include isolation and classification of microorganisms, metagenomics approaches, biochemistry of key metabolic enzymes, gene regulation and genetic systems, and field measurements. The state of the art techniques described here will both guide researchers in specific pursuits and educate the wider scientific community about this exciting and rapidly developing field. Topics include isolation and classification of microorganisms, metagenomics approaches, biochemistry of key metabolic enzymes, gene regulation and genetic systems, and field measurements The state-of-the-art techniques described here will both guide researchers in specific pursuits and educate the wider scientific community about this exciting and rapidly developing field. | ||
| 505 | 0 | |a 1.Characterization of the Recombinant Pyrophosphate-Dependent 6-Phosphofructokinases from Methylomicrobium alcaliphilum 20Z and Methylococcus capsulatus Bath / Yuri A. Trotsenko -- 1.Introduction -- 2.Methods -- 3.Brief Overview of Current Knowledge of PPi-PFK in Microorganisms -- 4.Properties of PPi-PFK from Methanotrophs -- 5.Conclusion -- Acknowledgments -- References -- 2.Genes and Enzymes of Ectoine Biosynthesis in Halotolerant Methanotrophs / Yuri A. Trotsenko -- 1.Introduction -- 2.PCR-Based Approach for Identification of the Ectoine Biosynthesis Genes in Methanotrophs -- 3.Transcriptional Regulation of the Ectoine Biosynthesis Genes -- 4.Key Enzymes of Ectoine Biosynthesis -- 5.Conclusion -- Acknowledgments -- References -- 3.Facultative and Obligate Methanotrophs: How to Identify and Differentiate Them / Peter F. Dunfield -- 1.Introduction -- 2.Identification of Methanotrophic Capabilities in Novel Isolates -- 3.Substrate Utilization Tests -- 4.Tests for Culture Purity -- References -- 4.Approaches for the Characterization and Description of Novel Methanotrophic Bacteria / John P. Bowman -- 1.Introduction to Aerobic Methanotroph Characterization for Systematic Research and Other Purposes -- 2.Broad Level Systematic Diversity -- 3.Methodological Approaches -- 4.Concluding Remarks -- References -- 5.Methylococcus capsulatus (Bath): From Genome to Protein Function, and Vice Versa / Harald B. Jensen -- 1.Introduction -- 2.Mapping of the Outer Membrane Proteome of M. capsulatus -- 3.Inner Membrane Protein Complexes Studied with Blue-Native Polyacrylamide Gel Electrophoresis (BN-PAGE) -- 4.Visualization and Identification of M. capsulatus c-type Heme Proteins -- Acknowledgments -- References -- 6.Functional Metagenomics of Methylotrophs / Ludmila Chistoserdova -- 1.Introduction -- 2.Enrichment for Specific Functional Types Using SIP -- 3.Creating a Metagenomic Database and Linking It to Functionality -- 4.Ultrashort Read-Based Metatranscriptomics -- 5.Conclusions and Future Perspectives -- Acknowledgments -- References -- 7.Genetic Systems for Moderately Halo(alkali)philic Bacteria of the Genus Methylomicrobium / Marina G. Kalyuzhnaya -- 1.Introduction -- 2.Methods -- 3.Outlook -- Acknowledgments -- References -- 8.Development of a System for Genetic Manipulation of the Facultative Methanotroph Methylocella silvestris BL2 / J. Colin Murrell -- 1.Introduction -- 2.Growth of M. silvestris -- 3.Introduction of Plasmid DNA by Conjugation and Electroporation -- 4.Gene Deletion by Electroporation of Linear DNA -- 5.Preparation of Competent Cells for Electroporation of M. silvestris -- 6.Construction of Linear DNA for Gene Deletion by Homologous Recombination -- 7.Electroporation -- 8.Efficiency of Gene Deletion -- 9.Case Study: Deletion of Isocitrate Lyase -- 10.Complementation -- 11.Phenotype of the Isocitrate Lyase Deletion Mutant -- 12.Conclusions -- References -- 9.Mutagenesis of Soluble Methane Monooxygenase / J. Colin Murrell -- 1.Introduction -- 2.Bacteriological Growth Media and Antibiotics -- 3.Mutagenesis and Subcloning -- 4.Expression Hosts and Conjugation -- 5.Analysis of Mutants -- Acknowledgments -- References -- 10.Single Cell Methods for Methane Oxidation Analysis / Mary E. Lidstrom -- 1.Introduction -- 2.Methods -- Acknowledgment -- References -- 11.Methanotroph Outer Membrane Preparation / Anne Fjellbirkeland -- 1.Introduction -- 2.Isolation of OMs -- 3.Enrichment of Integral and Tightly Associated OMPs -- 4.Extraction of Surface-Associated Proteins -- 5.Biotin Labeling of Surface-Exposed OMPs -- References -- 12.Overexpression and Purification of the Particulate Methane Monooxygenase from Methylococcus capsulatus (Bath) / Steve S.-F. Yu -- 1.Introduction -- 2.Overproduction of pMMO -- 3.Isolation and Purification of pMMO from the pMMO-Enriched Membranes -- 4.Characterization of the Purified pMMO-Detergent Complex -- Acknowledgments -- References -- 13.Metal Reconstitution of Particulate Methane Monooxygenase and Heterologous Expression of the pmoB Subunit / Amy C. Rosenzweig -- 1.Introduction -- 2.Preparation of apo pMMO Membranes and Metal Reconstitution -- 3.Soluble Domain Constructs of the pmoB Subunit -- Acknowledgments -- References -- 14.Particulate Methane Monooxygenase from Methylosinus trichosporium OB3b / Akimitsu Miyaji -- 1.Introduction -- 2.Bacterial Cells Expressing pMMO -- 3.Membrane-Bound Form of pMMO -- 4.Purification of Detergent-Solubilized pMMO -- 5.Summary -- Acknowledgments -- References -- 15.Production, Isolation, Purification, and Functional Characterization of Methanobactins / Hyung J. Kim -- 1.Introduction: Copper, Siderophores, Chalkophores, and Methanobactin -- 2.Methanotroph Growth and Optimizing Methanobactin Production -- 3.Methanobactin Isolation and Purification -- 4.Studying Methanobactins in Pseudonatural Environments -- Acknowledgments -- References -- 16.A Simple Assay for Screening Microorganisms for Chalkophore Production / Jeremy D. Semrau -- 1.Introduction -- 2.Cu-CAS Assay for Chalkophore Detection -- 3.Fe-CAS Assay for Detecting Nonspecific Binding of Copper from Cu-CAS by a Siderophore -- 4.Conclusions -- Acknowledgments -- References -- 17.Isolation of Methanobactin from the Spent Media of Methane-Oxidizing Bacteria / Alan A. DiSpirito -- 1.Introduction -- 2.Isolation of Methanobactin from the Spent Media of MOB -- 3.Purification of mb -- 4.Sample Variability -- Acknowledgments. | |
| 650 | 0 | |a Methanobacteriaceae. | |
| 650 | 0 | |a Methane |x Metabolism. | |
| 650 | 2 | |a Methanobacteriaceae |0 (DNLM)D017013 | |
| 650 | 6 | |a M�ethanobact�eriac�ees. |0 (CaQQLa)201-0278020 | |
| 650 | 6 | |a M�ethane |0 (CaQQLa)201-0023203 |x M�etabolisme. |0 (CaQQLa)201-0379985 | |
| 650 | 7 | |a Methane |x Metabolism. |2 fast |0 (OCoLC)fst01018574 | |
| 650 | 7 | |a Methanobacteriaceae. |2 fast |0 (OCoLC)fst01018586 | |
| 700 | 1 | |a Rosenzweig, Amy C. |q (Amy Claire) | |
| 700 | 1 | |a Ragsdale, Stephen W. | |
| 776 | 0 | 8 | |i Print version: |t Methods in methane metabolism. Part B, Methanotrophy. |d Amsterdam, Netherlands ; Boston, Mass. : Elsevier/ Academic Press, �2011 |z 9780123869050 |w (OCoLC)704379301 |
| 830 | 0 | |a Methods in enzymology ; |v volume 495. | |
| 856 | 4 | 0 | |u https://sciencedirect.uam.elogim.com/science/book/9780123869050 |z Texto completo |
| 856 | 4 | 0 | |u https://sciencedirect.uam.elogim.com/science/bookseries/00766879/495 |z Texto completo |
| 856 | 4 | 0 | |u https://sciencedirect.uam.elogim.com/science/bookseries/00766879/495 |z Texto completo |