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Angiogenesis. in vitro systems / Volume 443 :
Angiogenesis is the growth of new blood vessels and is an important natural process in the body. A healthy body maintains a perfect balance of angiogenesis modulators. In many serious disease states, however, the body loses control over antiogenesis. Diseases that are angiogensis-dependent result wh...
| Clasificación: | Libro Electrónico |
|---|---|
| Otros Autores: | |
| Formato: | Electrónico eBook |
| Idioma: | Inglés |
| Publicado: |
Amsterdam ; San Diego :
Elsevier/Academic Press,
�2008.
|
| Colección: | Methods in enzymology ;
volume 443. |
| Temas: | |
| Acceso en línea: | Texto completo Texto completo |
MARC
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| 245 | 0 | 0 | |a Angiogenesis. |n Volume 443 : |b in vitro systems / |c edited by David A. Cheresh. |
| 260 | |a Amsterdam ; |a San Diego : |b Elsevier/Academic Press, |c �2008. | ||
| 300 | |a 1 online resource (xlii, 356 pages, 4 unnumbered pages of plates) : |b illustrations (some color) | ||
| 336 | |a text |b txt |2 rdacontent | ||
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| 490 | 1 | |a Methods in enzymology, |x 0076-6879 ; |v v. 443 | |
| 504 | |a Includes bibliographical references and indexes. | ||
| 588 | 0 | |a Print version record. | |
| 520 | |a Angiogenesis is the growth of new blood vessels and is an important natural process in the body. A healthy body maintains a perfect balance of angiogenesis modulators. In many serious disease states, however, the body loses control over antiogenesis. Diseases that are angiogensis-dependent result when blood vessels either grow excessively or insufficiently. * Tried-and-tested techniques written by researchers that developed them, used them, and brought them to fruition * Provides the "builder's manual" for essential techniques--a one-stop shop that eliminates needless searching among untested techniques * Includes step-by-step methods for understanding the cell and molecular basis of wound healing, vascular integrin signaling, mechanical signaling in blood vessels, and vascular proteomics | ||
| 505 | 0 | |a Cover; Copyright Page; TOCContents; Contributors; Preface; Volumes in Series; CHChapter 1: Approaches for Studying Angiogenesis-Related Signal Transduction; 1. Introduction; 2. Angiogenesis; 3. Phosphorylation of eNOS on Ser1179; 4. Measuring Released NO by No-Specific Chemiluminescence; 5. Concluding Remarks; References; CHChapter 2: Physiologic Stress-Mediated Signaling in the Endothelium; 1. Introduction; 2. Parallel Plate Flow System; 3. Cone and Plate Flow System; 4. Cyclic Stretching of the Endothelium; Acknowledgments; References. | |
| 505 | 8 | |a CHChapter 3: Endothelial Cell Adhesion and Migration1. Introduction; 2. Cell Preparation; 3. Preparation of Well-Defined Surfaces; 4. Preparation of Polyacrylamide Substrates for Cell Adhesion Studies; 5. Quantifying Cell Adhesion; 6. Quantifying Endothelial Cell Migration; References; CHChapter 4: An Optimized Three-Dimensional In Vitro Model for the Analysis of Angiogenesis; 1. Overview; 2. Fibrin Bead Assay; 3. Immunocytochemistry of Angiogenic Sprouts In Vitro; 4. Retroviral Transduction of EC; 5. Laser Capture Microdissection; 6. Discussion; References. | |
| 505 | 8 | |a CHChapter 5: In Vitro Three Dimensional Collagen Matrix Models of Endothelial Lumen Formation During Vasculogenesis and Angiogenesis1. Introduction; 2. In Vitro Models of Vasculogenesis and Angiogenesis in 3D Collagen Matrices; 3. Molecular/Genetic Manipulation of ECs; 4. Rho GTPase Activation Assay During EC Tube Morphogenesis in 3D Collagen Matrices; 5. Biochemical Analysis of Interactions Between Cdc42 and its Downstream Effectors During EC Lumen and Tube Formation in 3D Colla; 6. Conclusions; Acknowledgments; References. | |
| 505 | 8 | |a CHChapter 6: In Vitro Differentiation of Mouse Embryonic Stem Cells Into Primitive Blood Vessels1. Introduction; 2. Maintenance of ES Cells; 3. Generation of Transgenic ES Cell Lines; 2. Concluding Remarks; Acknowledgments; References; CHChapter 7: The Aortic Ring Model of Angiogenesis; 1. Introduction; 2. Aortic Ring Angiogenesis Model: Basic Protocol; 3. Protocols for Analysis of Aortic Cultures; 4. Modifications of Basic Protocol; 5. Troubleshooting; 6. Concluding Remarks; References; CHChapter 8: An Assay System for In Vitro Detection of Permeability in Human ""Endothelium"" | |
| 505 | 8 | |a 1. Introduction2. Generation of a Human Endothelium Model for Permeability Studies; 3. Conclusion; Acknowledgments; References; CHChapter 9: Assays of Transendothelial Migration In Vitro; 1. Introduction; 2. Methods for Investigating Leukocyte Transendothelial Migration Under Static or Flow Conditions In Vitro; Acknowledgments; References; CHChapter 10: Cell Mechanics at Multiple Scales; 1. Introduction; 2. Large-Scale Cell Stretching; 3. Magnetic Micromanipulation; 4. Fluorescence Laser Tracking Microrheology; 5. General Summary and Conclusion; Acknowledgments; References. | |
| 650 | 0 | |a Neovascularization. | |
| 650 | 1 | 2 | |a Neovascularization, Physiologic |0 (DNLM)D018919 |
| 650 | 2 | 2 | |a Cell Culture Techniques |x methods |0 (DNLM)D018929Q000379 |
| 650 | 2 | |a Neovascularization, Pathologic |0 (DNLM)D009389 | |
| 650 | 6 | |a N�eovascularisation. |0 (CaQQLa)201-0162856 | |
| 650 | 7 | |a MEDICAL |x Physiology. |2 bisacsh | |
| 650 | 7 | |a Neovascularization |2 fast |0 (OCoLC)fst01035916 | |
| 650 | 7 | |a Angiogenese |2 gnd |0 (DE-588)4142449-9 | |
| 653 | 0 | 0 | |a angiogenese |
| 653 | 0 | 0 | |a angiogenesis |
| 653 | 0 | 0 | |a bloedvaten |
| 653 | 0 | 0 | |a blood vessels |
| 653 | 1 | 0 | |a Biochemistry |
| 653 | 1 | 0 | |a Biochemie |
| 700 | 1 | |a Cheresh, David A. | |
| 776 | 0 | 8 | |i Print version: |t Angiogenesis. |d Amsterdam ; San Diego : Elsevier/Academic Press, �2008 |z 9780123743152 |w (DLC) 2009285146 |w (OCoLC)422748054 |
| 830 | 0 | |a Methods in enzymology ; |v volume 443. | |
| 856 | 4 | 0 | |u https://sciencedirect.uam.elogim.com/science/book/9780123743152 |z Texto completo |
| 856 | 4 | 0 | |u https://sciencedirect.uam.elogim.com/science/bookseries/00766879/443 |z Texto completo |