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Pulsed field gel electrophoresis : a practical guide /
Pulsed Field Gel Electrophoresis: A Practical Guide is the first laboratory manual to describe the theory and practice of this technique. Based on the authors' experience developing pulsed field gel instruments and teaching procedures, this book provides everything a researcher or student needs...
| Clasificación: | Libro Electrónico |
|---|---|
| Autor principal: | |
| Otros Autores: | |
| Formato: | Electrónico eBook |
| Idioma: | Inglés |
| Publicado: |
San Diego :
Academic Press,
�1993.
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| Temas: | |
| Acceso en línea: | Texto completo |
Tabla de Contenidos:
- Introduction to conventional and pulsed field gel electrophoresis
- Setting up a pulsed field gel electrophoresis system
- Preparation of DNA for pulsed field analysis
- Pouring gels and handling samples
- Restriction enzyme digestion of large DNA fragments in agarose
- Switch intervals and resolution in pulsed field gels
- Field inversion gel electrophoresis
- Variables that affect pulsed field gels
- Southern blot analysis of pulsed field gels
- Special applications
- Trouble shooting
- Schematic diagram of the chef system
- Schematic diagram of the fige system
- Schematic diagram of the electrical circuit for avfige.