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Pulsed field gel electrophoresis : a practical guide /
Pulsed Field Gel Electrophoresis: A Practical Guide is the first laboratory manual to describe the theory and practice of this technique. Based on the authors' experience developing pulsed field gel instruments and teaching procedures, this book provides everything a researcher or student needs...
| Clasificación: | Libro Electrónico |
|---|---|
| Autor principal: | |
| Otros Autores: | |
| Formato: | Electrónico eBook |
| Idioma: | Inglés |
| Publicado: |
San Diego :
Academic Press,
�1993.
|
| Temas: | |
| Acceso en línea: | Texto completo |
MARC
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| 100 | 1 | |a Birren, Bruce W. | |
| 245 | 1 | 0 | |a Pulsed field gel electrophoresis : |b a practical guide / |c Bruce Birren, Eric Lai. |
| 260 | |a San Diego : |b Academic Press, |c �1993. | ||
| 300 | |a 1 online resource (xviii, 253 pages) : |b illustrations | ||
| 336 | |a text |b txt |2 rdacontent | ||
| 337 | |a computer |b c |2 rdamedia | ||
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| 504 | |a Includes bibliographical references (pages 199-244) and index. | ||
| 505 | 0 | |a Introduction to conventional and pulsed field gel electrophoresis -- Setting up a pulsed field gel electrophoresis system -- Preparation of DNA for pulsed field analysis -- Pouring gels and handling samples -- Restriction enzyme digestion of large DNA fragments in agarose -- Switch intervals and resolution in pulsed field gels -- Field inversion gel electrophoresis -- Variables that affect pulsed field gels -- Southern blot analysis of pulsed field gels -- Special applications -- Trouble shooting -- Schematic diagram of the chef system -- Schematic diagram of the fige system -- Schematic diagram of the electrical circuit for avfige. | |
| 506 | |3 Use copy |f Restrictions unspecified |2 star |5 MiAaHDL | ||
| 533 | |a Electronic reproduction. |b [Place of publication not identified] : |c HathiTrust Digital Library, |d 2010. |5 MiAaHDL | ||
| 538 | |a Master and use copy. Digital master created according to Benchmark for Faithful Digital Reproductions of Monographs and Serials, Version 1. Digital Library Federation, December 2002. |u http://purl.oclc.org/DLF/benchrepro0212 |5 MiAaHDL | ||
| 583 | 1 | |a digitized |c 2010 |h HathiTrust Digital Library |l committed to preserve |2 pda |5 MiAaHDL | |
| 520 | |a Pulsed Field Gel Electrophoresis: A Practical Guide is the first laboratory manual to describe the theory and practice of this technique. Based on the authors' experience developing pulsed field gel instruments and teaching procedures, this book provides everything a researcher or student needs to know in order to understand and carry out pulsed field gel experiments. Clear, well-tested protocols assume only that users have a basic familiarity with molecular biology. Thorough coverage of useful data, theory, and applications ensures that this book is also a lasting resource for more advanced practitioners of pulsed field gels. Key Features * Reviews all types of pulsed field gel electrophoresis * Describes all commercially available systems and summarizes advantages and limitations of each * Includes step-by-step protocols for sample preparation and analysis * Illustrated with photographs that depict * How to run gels * What the results should look like * What they look like when they go wrong * Covers applications to a wide range of organisms * Includes bibliography of over 900 publications and cross-referenced by topic, application, and organism. | ||
| 588 | 0 | |a Print version record. | |
| 650 | 0 | |a Pulsed-field gel electrophoresis |x Technique |v Handbooks, manuals, etc. | |
| 650 | 0 | |a DNA |x Analysis |v Handbooks, manuals, etc. | |
| 650 | 0 | |a Pulsed-field gel electrophoresis. | |
| 650 | 2 | |a Electrophoresis, Gel, Pulsed-Field |0 (DNLM)D016521 | |
| 650 | 2 | |a DNA |x analysis |0 (DNLM)D004247Q000032 | |
| 650 | 6 | |a �Electrophor�ese en champ puls�e |0 (CaQQLa)201-0282363 |x Technique |0 (CaQQLa)201-0377564 |v Guides, manuels, etc. |0 (CaQQLa)201-0377046 | |
| 650 | 6 | |a ADN |0 (CaQQLa)201-0008510 |x Analyse |0 (CaQQLa)201-0374819 |v Guides, manuels, etc. |0 (CaQQLa)201-0377046 | |
| 650 | 6 | |a �Electrophor�ese en champ puls�e. |0 (CaQQLa)201-0282363 | |
| 650 | 7 | |a Pulsed-field gel electrophoresis |2 fast |0 (OCoLC)fst01083955 | |
| 650 | 7 | |a DNA |x Analysis |2 fast |0 (OCoLC)fst00886556 | |
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| 653 | 0 | |a Electrochemistry | |
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| 700 | 1 | |a Lai, Eric Hon Cheong, |d 1957- | |
| 776 | 0 | 8 | |i Print version: |a Birren, Bruce W. |t Pulsed field gel electrophoresis. |d San Diego : Academic Press, �1993 |w (DLC) 93191440 |w (OCoLC)31865767 |
| 856 | 4 | 0 | |u https://sciencedirect.uam.elogim.com/science/book/9780121012908 |z Texto completo |