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Recombinant DNA laboratory manual /
The latest edition of this introductory benchtop manual is up-to-date, affordable, and easy-to-follow. This text is perfect for your two-quarter or one semester course in Recombinant DNA Techniques and is specifically designed to lead your student or technician, who is a newcomer to molecular biolog...
| Clasificación: | Libro Electrónico |
|---|---|
| Autor principal: | |
| Otros Autores: | |
| Formato: | Electrónico eBook |
| Idioma: | Inglés |
| Publicado: |
San Diego :
Academic Press,
�1992.
|
| Edición: | Rev. ed. |
| Temas: | |
| Acceso en línea: | Texto completo |
MARC
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| 100 | 1 | |a Zyskind, Judith W. | |
| 245 | 1 | 0 | |a Recombinant DNA laboratory manual / |c Judith W. Zyskind, Sanford I. Bernstein. |
| 250 | |a Rev. ed. | ||
| 260 | |a San Diego : |b Academic Press, |c �1992. | ||
| 300 | |a 1 online resource (xiv, 224 pages) : |b illustrations | ||
| 336 | |a text |b txt |2 rdacontent | ||
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| 504 | |a Includes bibliographical references and index. | ||
| 506 | |3 Use copy |f Restrictions unspecified |2 star |5 MiAaHDL | ||
| 533 | |a Electronic reproduction. |b [Place of publication not identified] : |c HathiTrust Digital Library, |d 2010. |5 MiAaHDL | ||
| 538 | |a Master and use copy. Digital master created according to Benchmark for Faithful Digital Reproductions of Monographs and Serials, Version 1. Digital Library Federation, December 2002. |u http://purl.oclc.org/DLF/benchrepro0212 |5 MiAaHDL | ||
| 583 | 1 | |a digitized |c 2010 |h HathiTrust Digital Library |l committed to preserve |2 pda |5 MiAaHDL | |
| 588 | 0 | |a Print version record. | |
| 520 | |a The latest edition of this introductory benchtop manual is up-to-date, affordable, and easy-to-follow. This text is perfect for your two-quarter or one semester course in Recombinant DNA Techniques and is specifically designed to lead your student or technician, who is a newcomer to molecular biology, from the basic skills of growing and maintaining bacterial colonies through plasmid DNA isolation, cloning, DNA sequencing, and hybrid detection. The most useful features of this manual for your classroom and laboratory are: * Comb-bound, three-column, large 9-1/4" x 7-1/2" format * Exercises contain explanatory material and margin notes that pinpoint critical steps and important concepts * Necessary reagents and equipment are presented in a checklist at the beginning of each protocol * Techniques for bacteria are complemented with those for Drosophila * Each experiment has been tested in the laboratory by students for five years * Features a complete chapter on computers in the molecular biology laboratory * Presents helpful appendixes on safety in the laboratory, frequently used ancillary techniques, and recipes for buffers, media, and strains | ||
| 505 | 0 | |a Front Cover; Copyright Page; Recombinant DNA Laboratory Manual; Dedication; Table of Contents; PREFACE; SCHEDULE OF LABORATORY EXERCISES; Lab I. BACTERIAL GROWTH PARAMETERS; Day 1. Measuring Bacterial Cell Growth; Day 2. Plotting Cell Growth Data; Addendum; Lab II. Isolation and Analysis of Bacterial and Drosophila Chromosomal DNA; Day 1. Isolation and Purification of E. coli Chromosomal DNA; Day 2. Determination of the Concentration and Purity of DNA by UV Spectroscopy; Day 3. Restriction Endonuclease Digestion of Chromosomal DNA | |
| 505 | 8 | |a Day 4. Agarose Gel of Chromosomal DNA Restriction Endonuclease DigestionsDay 5. Staining and Photography of Agarose Gel of Chromosomal DNA Restriction Endonuclease Digestions; Lab III. Plasmid DNA Isolation and Agarose Gel Analysis; Day 1. Isolation of Plasmid DNA by the Alkaline-Detergent Method- A Miniprep Procedure; Day 2. Agarose Gel Electrophoresis of Undigested Plasmid DNA; Lab IV. Introduction of DNA into Cells; Day 1. Production of Frozen Competent Cells; Day 2. Transformation of LE392 with pBR329 DNA Isolated from HB101 ::Tn5; Lab V. Tn5 Mutagenesis of PBR329 | |
| 505 | 8 | |a Day 1. Marker Screening: Divide Transformants into Tcs and Tcr ClassesDay 2. Purification of Tcs and Tcr Clones; Day 3. Isolation of Plasmid DNA by the Alkaline-Detergent Method and Determination of Recovery by Agarose Gel Electrophoresis; Day 4. Restriction Mapping of the Tn5 Inserts using Pstl and EcoRl; Day 5. Agarose Gel of Plasmid DNA Restriction Endonuclease Digestions; Lab VI. DNA Cloning in M13; Day 1. Isolation of Restriction Fragment from an Agarose Gel; Day 2. Estimation of Recovery of Restriction Fragment and Isolation of M13mp 19 RF DNA | |
| 505 | 8 | |a Day 3. EcoRl Digestion of M13mpl9 RF DNA and Treatment with Alkaline PhosphataseDay 4. Removing the Phosphatase and EcoRl and Analysis of the EcoRl Digest of M13mpl9 RF DNA; Day 5. Ligation; Ligation of EcoRl Digested M13mpl9 RF DNA and the Purified pRSG192 EcoRl Fragment; Day 6. Transfection of XL 1-Blue with Ligation Mixtures; Day 7. Plaque Purification; Day 8. Isolation of Colorless Plaques That Contain the chb Gene and Growth of Recombinant Bacteriophage; Lab VII. DNA Sequencing; Day 1. Isolation of Recombinant M13mpl9 RF and ss DNA | |
| 505 | 8 | |a Day 2. Restriction Digestion and Gel Electrophoresis of Recombinant Phage to Determine Orientation of InsertEstimating the Recovery of ss DNA using Agarose Gel Electrophoresis; Day 3. Staining Gel of Pstl Restriction Fragments; Template Annealing and Dideoxy Sequencing; Day 4. Electrophoretic Separation of Sequencing Reactions; Day 5. Developing Autoradiogram and Reading DNA Sequence; Lab VIII. DNA Gel Blotting, Probe Preparation, Hybridization, and Hybrid Detection; Day 0. Agarose Gel Electrophoresis; Day 1. Gel Blotting; Day 2. Baking the Blot, Nick Translation, and Biotinylation of DNA | |
| 546 | |a English. | ||
| 650 | 0 | |a Recombinant DNA |v Laboratory manuals. | |
| 650 | 0 | |a Recombinant DNA. | |
| 650 | 2 | |a DNA, Recombinant |0 (DNLM)D004274 | |
| 650 | 6 | |a ADN recombinant |0 (CaQQLa)201-0024828 |v Manuels de laboratoire. |0 (CaQQLa)201-0376994 | |
| 650 | 6 | |a ADN recombinant. |0 (CaQQLa)201-0024828 | |
| 650 | 7 | |a Recombinant DNA |2 fast |0 (OCoLC)fst01091466 | |
| 650 | 7 | |a Methode |2 gnd |0 (DE-588)4038971-6 | |
| 650 | 7 | |a Molekularbiologie |2 gnd |0 (DE-588)4039983-7 | |
| 650 | 7 | |a Rekombinante DNS |2 gnd |0 (DE-588)4225578-8 | |
| 650 | 1 | 7 | |a Recombinant DNA. |2 gtt |
| 650 | 1 | 7 | |a Laboratoriumonderzoek. |2 gtt |
| 650 | 1 | 7 | |a Genetische manipulatie. |2 gtt |
| 653 | 0 | |a DNA | |
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| 700 | 1 | |a Bernstein, Sanford I. | |
| 776 | 0 | 8 | |i Print version: |a Zyskind, Judith W. |t Recombinant DNA laboratory manual. |b Rev. ed. |d San Diego : Academic Press, �1992 |w (DLC) 91040989 |w (OCoLC)24699974 |
| 856 | 4 | 0 | |u https://sciencedirect.uam.elogim.com/science/book/9780127844015 |z Texto completo |