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Gel electrophoresis of proteins /
Gel Electrophoresis of Proteins.
| Clasificación: | Libro Electrónico |
|---|---|
| Otros Autores: | |
| Formato: | Electrónico eBook |
| Idioma: | Inglés |
| Publicado: |
Bristol :
Wright,
1986.
|
| Temas: | |
| Acceso en línea: | Texto completo |
MARC
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| 245 | 0 | 0 | |a Gel electrophoresis of proteins / |c edited by M.J. Dunn. |
| 260 | |a Bristol : |b Wright, |c 1986. | ||
| 300 | |a 1 online resource (xiii, 407 pages) : |b illustrations | ||
| 336 | |a text |b txt |2 rdacontent | ||
| 337 | |a computer |b c |2 rdamedia | ||
| 338 | |a online resource |b cr |2 rdacarrier | ||
| 504 | |a Includes bibliographical references and indexes. | ||
| 506 | |3 Use copy |f Restrictions unspecified |2 star |5 MiAaHDL | ||
| 533 | |a Electronic reproduction. |b [Place of publication not identified] : |c HathiTrust Digital Library, |d 2010. |5 MiAaHDL | ||
| 538 | |a Master and use copy. Digital master created according to Benchmark for Faithful Digital Reproductions of Monographs and Serials, Version 1. Digital Library Federation, December 2002. |u http://purl.oclc.org/DLF/benchrepro0212 |5 MiAaHDL | ||
| 583 | 1 | |a digitized |c 2010 |h HathiTrust Digital Library |l committed to preserve |2 pda |5 MiAaHDL | |
| 588 | 0 | |a Print version record. | |
| 505 | 0 | |a Front Cover; Gel Electrophoresis of Proteins; Copyright Page; Preface; List of Contributors; Table of Contents; List of Abbreviations; Chapter 1. Steady-state Gel Electrophoresis Systems; 1.1 Introduction; 1.2 Historical Developments; 1.3 Fundamental Steady-state Electrophoresis Systems; 1.4 Fundamental Properties of Steady-state Electrophoresis Systems; 1.5 Nomenclature and Definitions; 1.6 Conductivity; 1.7 Equations for Calculation of System Composition; 1.8 Isotachophoresis; 1.9 Moving Boundary Electrophoresis; 1.10 Isoelectric Focusing Systems; Acknowledgements; References | |
| 505 | 8 | |a Chapter 2. One-dimensional PAA-gel Electrophoretic Techniques to Separate Functional and Denatured Proteins2.1 Introduction; 2.2 Structure and Physico-chemical Properties of Polyacrylamide Gels; 2.3 Analytical Polyacrylamide Gel Electrophoresis; 2.4 Affinity Electrophoresis; Acknowledgments; References; Chapter 3. Conventional Isoelectric Focusing and Immobilised pH Gradients; 3.1 Introduction; 3.2 The Principles of IEF; 3.3 IEF in Agarose Matrices; 3.4 IEF in Polyacrylamide Matrices; 3.5 Titration Curves; 3.6 Production of Narrow pH Gradients; 3.7 Measurements of pH Gradients | |
| 505 | 8 | |a 3.8 Trouble Shooting3.9 Artefacts: a Unified View; 3.10 The Chemicals; the Immobiline Matrix; 3.11 Narrow and Ultra-narrow pH Gradients; 3.12 Extended pH Gradients; 3.13 On Buffering Capacity and Ionic Strength; 3.14 On Electro-endosmosis; 3.15 Polymerisation Kinetics; 3.16 Methodology: Casting an Immobiline Gel; 3.17 Artefacts; 3.18 Conclusions; Acknowledgments; References; Chapter 4. High Resolution Two-dimensional Polyacrylamide-gel Electrophoresis; 4.1 Introduction; 4.2 Two-dimensional Electrophoresis under Non-denaturing Conditions | |
| 505 | 8 | |a 4.3 Denaturing 2D Systems using Electrophoresis in both Dimensions4.4 The O'Farrell System; 4.5 Sample Preparation and Solubilisation; 4.6 IEF Dimension; 4.7 New Alternatives for the First Dimension of 2D PAGE; 4.8 Equilibration; 4.9 SDS Dimension; 4.10 Visualisation, Detection and Analysis; 4.11 Heterogeneity and Artefacts; 4.12 Estimates of Resolution; 4.13 Applications; 4.14 2D-PAGE and the Study of Human Genetic Disorders; Acknowledgments; References; Chapter 5. Immunoelectrophoretic Methods; 5.1 Introduction; 5.2 Historical Background; 5.3 Theory; 5.4 General Conditions and Guidelines | |
| 505 | 8 | |a 5.5 Contemporary Methods5.6 Applicability of Immunoelectrophoretic Methods; 5.7 Advantages and Drawbacks; References; Chapter 6. In situ Peptide Mapping of Proteins and Polypeptides Separated by Polyacrylamide Gel Electrophoresis; 6.1 Introduction; 6.2 Primary Gel Systems; 6.3 Storage, Rehydration and Equilibration; 6.4 Protein Cleavage; 6.5 Secondary Gel Systems; 6.6 Detection; 6.7 Evaluation; References; Chapter 7. Protein Staining and Detection Methods; 7.1 Historical Introduction; 7.2 Post-Electrophoretic Organic Protein Stains; 7.3 Pre-electrophoretic Organic Stains; 7.4 Silver Stains | |
| 520 | |a Gel Electrophoresis of Proteins. | ||
| 650 | 0 | |a Proteins |x Purification. | |
| 650 | 0 | |a Gel electrophoresis. | |
| 650 | 0 | |a Electrophoresis. | |
| 650 | 0 | |a Proteins |x Analysis. | |
| 650 | 2 | |a Electrophoresis, Polyacrylamide Gel |x methods |0 (DNLM)D004591Q000379 | |
| 650 | 2 | |a Proteins |x analysis |0 (DNLM)D011506Q000032 | |
| 650 | 2 | |a Electrophoresis |0 (DNLM)D004586 | |
| 650 | 6 | |a �Electrophor�ese. |0 (CaQQLa)201-0007233 | |
| 650 | 6 | |a Prot�eines |x Analyse. |0 (CaQQLa)201-0031951 | |
| 650 | 7 | |a SCIENCE |x Life Sciences |x Biology. |2 bisacsh | |
| 650 | 7 | |a Proteins |x Analysis |2 fast |0 (OCoLC)fst01079714 | |
| 650 | 7 | |a Electrophoresis |2 fast |0 (OCoLC)fst00907684 | |
| 650 | 7 | |a Gel electrophoresis |2 fast |0 (OCoLC)fst00939503 | |
| 650 | 7 | |a Proteins |x Purification |2 fast |0 (OCoLC)fst01079750 | |
| 650 | 7 | |a Gelelektrophorese |2 gnd |0 (DE-588)4123267-7 | |
| 650 | 7 | |a Proteine |2 gnd |0 (DE-588)4076388-2 | |
| 650 | 7 | |a Prot�eines |x �Electrophor�ese. |2 ram | |
| 650 | 7 | |a �Electrophor�ese sur gel. |2 ram | |
| 653 | |a Proteins |a Chemical analysis |a Gel electrophoresis | ||
| 700 | 1 | |a Dunn, M. J. | |
| 776 | 0 | 8 | |i Print version: |t Gel electrophoresis of proteins. |d Bristol : Wright, 1986 |w (DLC) 86219615 |w (OCoLC)21906637 |
| 856 | 4 | 0 | |u https://sciencedirect.uam.elogim.com/science/book/9780723608820 |z Texto completo |