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101117s1993 caua ob 001 0 eng d |
| 040 |
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|a OCLCE
|b eng
|e pn
|c OCLCE
|d OCLCQ
|d OPELS
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|a 643888121
|a 842859981
|a 974618009
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|a 987731272
|a 988556507
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|a 1299193684
|q (ebk)
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|a 9781299193680
|q (ebk)
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|z 9780123133205
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|z 0123133203
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| 035 |
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|a (OCoLC)681973039
|z (OCoLC)643888121
|z (OCoLC)842859981
|z (OCoLC)974618009
|z (OCoLC)974669495
|z (OCoLC)987731272
|z (OCoLC)988556507
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| 042 |
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|a dlr
|
| 050 |
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4 |
|a QH442.2
|b .C46 1993
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| 060 |
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4 |
|a QH 583.2
|b H141c 1993
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| 082 |
0 |
4 |
|a 575.1/0724
|2 20
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| 084 |
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|a WC 4400
|2 rvk
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| 084 |
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|a WG 3450
|2 rvk
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| 084 |
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|a BIO 750f
|2 stub
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| 084 |
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|a BIO 180f
|2 stub
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| 084 |
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|a BIO 220f
|2 stub
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| 245 |
0 |
0 |
|a Chromosome microdissection and cloning :
|b a practical guide /
|c [edited by] Nabil G. Hagig and Michael V. Viola.
|
| 260 |
|
|
|a San Diego :
|b Academic Press,
|c 1993.
|
| 300 |
|
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|a 1 online resource (x, 160 pages) :
|b illustrations
|
| 336 |
|
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|a text
|b txt
|2 rdacontent
|
| 337 |
|
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|a computer
|b c
|2 rdamedia
|
| 338 |
|
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|a online resource
|b cr
|2 rdacarrier
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| 504 |
|
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|a Includes bibliographical references and index.
|
| 505 |
0 |
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|a Introduction to chromosome microdissection -- Chromosome organization -- Cloning DNA from chromosome fragments -- Preparation of chromosomes for microdissection -- Critical aspects of chromosome preparation -- Enrichment of metaphase spreads -- Hypotonic treatment -- Chromosome fixing and spreading -- Aging, storing, and staining of metaphase spreads -- Reagents -- Equipment -- Protocols -- Protocol 1. Preparation of chromosomes from peripheral blood T lymphocytes (whole blood microculture method) -- Protocol 2. Preparation of chromosomes from monolayer tissue culture cell lines -- Protocol 3. Preparation of chromosomes from monolayer cells grown on coverslips -- Protocol 4. Preparation of chromosomes from dipteran salivary glands -- Protocol 5. Solid staining and GTG banding of metaphase chromosomes -- Methods of chromosome microdissection -- Methods -- Video microscope method -- Oil chamber method -- Laser microdissection method -- Summary of chromosome microdissection and collection for DNA cloning -- Reagents and equipment -- Protocol.
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| 505 |
0 |
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|a (cont) Molecular cloning of microdissected chromosal DNA -- Cloning of DNA from microdissected chromosomal DNA fragments -- Method 1. Direct cloning of DNA from microdissected chromosomal fragments -- Direct cloning into [gamma] phage -- Method 2. Ligation of microdissected chromosomal DNA with plasmid vector or linker-adaptor and PCR amplification -- Protocol 2.1. Ligation of microdissected DNA with plasmid vector, PCR amplification, and cloning -- Protocol 2.2. Ligation of microdissected DNA with linker-adaptor, PCR amplification, and cloning -- Method 3. PCR amplification of microdissected chromosomal DNA fragments followed by probing a complete recombinant library -- Protocol 3.1. Preparation of chromosomal DNA for amplification -- Protocol 3.2. PCR amplification of microdissected chromosomal DNA using "universal" primers -- Protocol 3.3. PCR amplification using human Alu sequence-based primers -- Analysis of recombinant clones derived from microdissected chromosomal DNA -- Determination of DNA insert size range -- Determination of the percentage of recombinant clones containing repeat and unique sequences.
|
| 505 |
0 |
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|a (cont) Protocol 4.1. Assay for repeat sequences -- Protocol 4.2. Assay for unique sequences -- Calculation of the percentage of total microdissected DNA cloned -- Determination of potential structural gene sequences -- Localization of recombinant clones using in situ hybridization -- Protocol 5.1. DNA probe labeling for fluorescence in situ hybridization -- Protocol 5.2. Fluorescent in situ hybridization to metaphase chromosome spreads -- Applications of chromosome microdissection -- Direct analysis of the PCR product of microdissected chromosome fragments -- Gene mapping -- Mapping sites of chromosome rearrangement and deletions -- Determination of coupling phase -- Recombinant DNA libraries generated from microdissected chromosome fragments -- Genetic analysis of specialized chromosome structures -- Applications in genomic sequencing projects -- Characterization of disease-related genetic loci -- Study of chromosome abnormalities in cancer cells -- Gene transfer using chromosome fragments.
|
| 506 |
|
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|3 Use copy
|f Restrictions unspecified
|2 star
|5 MiAaHDL
|
| 533 |
|
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|a Electronic reproduction.
|b [Place of publication not identified] :
|c HathiTrust Digital Library,
|d 2010.
|5 MiAaHDL
|
| 538 |
|
|
|a Master and use copy. Digital master created according to Benchmark for Faithful Digital Reproductions of Monographs and Serials, Version 1. Digital Library Federation, December 2002.
|u http://purl.oclc.org/DLF/benchrepro0212
|5 MiAaHDL
|
| 583 |
1 |
|
|a digitized
|c 2010
|h HathiTrust Digital Library
|l committed to preserve
|2 pda
|5 MiAaHDL
|
| 588 |
0 |
|
|a Print version record.
|
| 650 |
|
0 |
|a Molecular cloning
|x Technique.
|
| 650 |
|
0 |
|a Microdissection.
|
| 650 |
|
0 |
|a Chromosomes.
|
| 650 |
|
2 |
|a Chromosomes
|x ultrastructure
|0 (DNLM)D002875Q000648
|
| 650 |
|
2 |
|a Cloning, Molecular
|x methods
|0 (DNLM)D003001Q000379
|
| 650 |
|
2 |
|a Dissection
|x methods
|0 (DNLM)D004210Q000379
|
| 650 |
|
2 |
|a Chromosomes
|0 (DNLM)D002875
|
| 650 |
|
6 |
|a Clonage mol�eculaire
|0 (CaQQLa)201-0032258
|x Technique.
|0 (CaQQLa)201-0377564
|
| 650 |
|
6 |
|a Microdissection.
|0 (CaQQLa)201-0169802
|
| 650 |
|
6 |
|a Chromosomes.
|0 (CaQQLa)201-0019407
|
| 650 |
|
7 |
|a Chromosomes
|2 fast
|0 (OCoLC)fst00860066
|
| 650 |
|
7 |
|a Microdissection
|2 fast
|0 (OCoLC)fst01019726
|
| 650 |
|
7 |
|a Molecular cloning
|x Technique
|2 fast
|0 (OCoLC)fst01024769
|
| 650 |
|
7 |
|a Chromosom
|2 gnd
|0 (DE-588)4010162-9
|
| 650 |
|
7 |
|a Mikromanipulator
|2 gnd
|0 (DE-588)4169834-4
|
| 653 |
0 |
|
|a Chromosomes
|a Analysis
|
| 655 |
|
2 |
|a Laboratory Manual
|0 (DNLM)D020484
|
| 655 |
|
7 |
|a Laboratory manuals
|2 fast
|0 (OCoLC)fst01920776
|
| 655 |
|
7 |
|a Laboratory manuals.
|2 lcgft
|
| 655 |
|
7 |
|a Manuels de laboratoire.
|2 rvmgf
|0 (CaQQLa)RVMGF-000001214
|
| 700 |
1 |
|
|a Hagag, Nabil G.
|
| 700 |
1 |
|
|a Viola, Michael V.
|
| 776 |
0 |
8 |
|i Print version:
|t Chromosome microdissection and cloning.
|d San Diego : Academic Press, 1993
|w (DLC) 93007286
|w (OCoLC)27816787
|
| 856 |
4 |
0 |
|u https://sciencedirect.uam.elogim.com/science/book/9780123133205
|z Texto completo
|
