GUS protocols : using the GUS gene as a reporter of gene expression /

The gb-glucuronidase (GUS) gene is extremely useful as a reporter of the expression of introduced genes and can be used in organisms where other reporter genes are useless. Thus, the GUS gene is the reporter gene of choice for transgenic plant research. Not only can this assay be used to detect whet...

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Detalles Bibliográficos
Clasificación:Libro Electrónico
Otros Autores: Gallagher, Sean R.
Formato: Electrónico eBook
Idioma:Inglés
Publicado: San Diego : Academic Press, �1992.
Temas:
Beta-glucuronidase genes.
Gene expression.
Biochemical markers.
Genetic engineering > Technique.
Molecular cloning.
Operons.
Cloning, Molecular
Glucuronidase > metabolism
Operon
Gene Expression
Biomarkers
Expression g�enique.
Marqueurs biologiques.
G�enie g�en�etique > Technique.
G�enes de la b�eta-glucuronidase.
Clonage mol�eculaire.
Op�erons.
Operons
Molecular cloning
Genetica.
Genetics
Acceso en línea:Texto completo
Tabla de Contenidos:
  • The Escherichia coli gus operon: introduction and expression of the gus operon in E. coli and the occurrence and use of GUS in other bacteria / Kate J. Wilson, Stephen G. Hughes, and Richard A. Jefferson
  • The GUS reporter system as a tool to study plant gene expression / Thomas Martin [and others]
  • Quantitation of GUS activity by fluorometry / Sean R. Gallagher
  • Histochemical, spectrophotometric, and fluorometric GUS substrates / John J. Naleway
  • Automated preparation of plant samples for enzymatic analysis / Thomas B. Brumback, Jr.
  • Microtiter plate-based assay for [beta]-d-glucuronidase: a quantitative approach / A. Gururaj Rao and Pamela Flynn
  • Histochemical localization of [beta]-glucuronidase / Anne-Marie Stomp
  • The GUS reporter gene: application to light and transmission electron microscopy / Stuart Craig
  • Review of the use of the GUS gene for analysis of secretory systems / Leigh B. Farrell and Roger N. Beachy
  • Applications of GUS to molecular plant virology / Jane K. Osbourn and T. Michael A. Wilson.
  • (cont) Sensitive assay for the excision of transposable elements using [beta]-glucuronidase reporter gene / E. Jean Finnegan
  • Anthocyanin genes as visual markers in transformed maize tissues / Ben Bowen
  • GUS as a useful reporter gene in animal cells / Daniel R. Gallie, John N. Feder, and Virginia Walbot
  • [beta]-glucuronidase (GUS) assay in animal tissue / John W. Kyle [and others].

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