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GUS protocols : using the GUS gene as a reporter of gene expression /
The gb-glucuronidase (GUS) gene is extremely useful as a reporter of the expression of introduced genes and can be used in organisms where other reporter genes are useless. Thus, the GUS gene is the reporter gene of choice for transgenic plant research. Not only can this assay be used to detect whet...
| Clasificación: | Libro Electrónico |
|---|---|
| Otros Autores: | |
| Formato: | Electrónico eBook |
| Idioma: | Inglés |
| Publicado: |
San Diego :
Academic Press,
�1992.
|
| Temas: | |
| Acceso en línea: | Texto completo |
MARC
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| 245 | 0 | 0 | |a GUS protocols : |b using the GUS gene as a reporter of gene expression / |c edited by Sean R. Gallagher. |
| 260 | |a San Diego : |b Academic Press, |c �1992. | ||
| 300 | |a 1 online resource (xxii, 221 pages, 6 unnumbered pages of plates) : |b illustrations (some color) | ||
| 336 | |a text |b txt |2 rdacontent | ||
| 337 | |a computer |b c |2 rdamedia | ||
| 338 | |a online resource |b cr |2 rdacarrier | ||
| 504 | |a Includes bibliographical references and index. | ||
| 505 | 0 | |a The Escherichia coli gus operon: introduction and expression of the gus operon in E. coli and the occurrence and use of GUS in other bacteria / Kate J. Wilson, Stephen G. Hughes, and Richard A. Jefferson -- The GUS reporter system as a tool to study plant gene expression / Thomas Martin [and others] -- Quantitation of GUS activity by fluorometry / Sean R. Gallagher -- Histochemical, spectrophotometric, and fluorometric GUS substrates / John J. Naleway -- Automated preparation of plant samples for enzymatic analysis / Thomas B. Brumback, Jr. -- Microtiter plate-based assay for [beta]-d-glucuronidase: a quantitative approach / A. Gururaj Rao and Pamela Flynn -- Histochemical localization of [beta]-glucuronidase / Anne-Marie Stomp -- The GUS reporter gene: application to light and transmission electron microscopy / Stuart Craig -- Review of the use of the GUS gene for analysis of secretory systems / Leigh B. Farrell and Roger N. Beachy -- Applications of GUS to molecular plant virology / Jane K. Osbourn and T. Michael A. Wilson. | |
| 505 | 0 | |a (cont) Sensitive assay for the excision of transposable elements using [beta]-glucuronidase reporter gene / E. Jean Finnegan -- Anthocyanin genes as visual markers in transformed maize tissues / Ben Bowen -- GUS as a useful reporter gene in animal cells / Daniel R. Gallie, John N. Feder, and Virginia Walbot -- [beta]-glucuronidase (GUS) assay in animal tissue / John W. Kyle [and others]. | |
| 506 | |3 Use copy |f Restrictions unspecified |2 star |5 MiAaHDL | ||
| 533 | |a Electronic reproduction. |b [Place of publication not identified] : |c HathiTrust Digital Library, |d 2010. |5 MiAaHDL | ||
| 538 | |a Master and use copy. Digital master created according to Benchmark for Faithful Digital Reproductions of Monographs and Serials, Version 1. Digital Library Federation, December 2002. |u http://purl.oclc.org/DLF/benchrepro0212 |5 MiAaHDL | ||
| 583 | 1 | |a digitized |c 2010 |h HathiTrust Digital Library |l committed to preserve |2 pda |5 MiAaHDL | |
| 520 | |a The gb-glucuronidase (GUS) gene is extremely useful as a reporter of the expression of introduced genes and can be used in organisms where other reporter genes are useless. Thus, the GUS gene is the reporter gene of choice for transgenic plant research. Not only can this assay be used to detect whether a gene is being expressed, but it can be used to determine the location of the gene product within cells. Low cost is another advantage of this assay. GUS Protocols provides instructions and essential background information that will enable researchers to effectively use the GUS gene as a reporter of the expression of introduced genes. Key Features * First book on the GUS reporter system * Up-to-date reference lists following each of the fourteen chapters * Comb-bound for convenient bench-top use * Written by leading authorities including R.A. Jefferson, inventor of the GUS assay * Illustrated with color * Easy-to-follow procedures for * Using the GUS system in both plants and animals * GUS detection by fluorometric, spectrophotometric, and histochemical methods * Fast, automated assays Background information on * The gb-glucuronidase reaction * Substrates * Instrumentation * Construction of GUS fusion vectors. | ||
| 588 | 0 | |a Print version record. | |
| 650 | 0 | |a Beta-glucuronidase genes. | |
| 650 | 0 | |a Gene expression. | |
| 650 | 0 | |a Biochemical markers. | |
| 650 | 0 | |a Genetic engineering |x Technique. | |
| 650 | 0 | |a Molecular cloning. | |
| 650 | 0 | |a Operons. | |
| 650 | 2 | |a Cloning, Molecular |0 (DNLM)D003001 | |
| 650 | 2 | |a Glucuronidase |x metabolism |0 (DNLM)D005966Q000378 | |
| 650 | 2 | |a Operon |0 (DNLM)D009876 | |
| 650 | 2 | |a Gene Expression |0 (DNLM)D015870 | |
| 650 | 2 | |a Biomarkers |0 (DNLM)D015415 | |
| 650 | 6 | |a Expression g�enique. |0 (CaQQLa)201-0028679 | |
| 650 | 6 | |a Marqueurs biologiques. |0 (CaQQLa)201-0177920 | |
| 650 | 6 | |a G�enie g�en�etique |0 (CaQQLa)201-0022662 |x Technique. |0 (CaQQLa)201-0377564 | |
| 650 | 6 | |a G�enes de la b�eta-glucuronidase. |0 (CaQQLa)201-0234925 | |
| 650 | 6 | |a Clonage mol�eculaire. |0 (CaQQLa)201-0032258 | |
| 650 | 6 | |a Op�erons. |0 (CaQQLa)201-0058949 | |
| 650 | 7 | |a Operons |2 fast |0 (OCoLC)fst01046427 | |
| 650 | 7 | |a Molecular cloning |2 fast |0 (OCoLC)fst01024762 | |
| 650 | 7 | |a Beta-glucuronidase genes. |2 fast |0 (OCoLC)fst00830908 | |
| 650 | 7 | |a Biochemical markers. |2 fast |0 (OCoLC)fst00831950 | |
| 650 | 7 | |a Gene expression. |2 fast |0 (OCoLC)fst00939613 | |
| 650 | 7 | |a Genetic engineering |x Technique. |2 fast |0 (OCoLC)fst00940060 | |
| 650 | 7 | |a Genetica. |2 larpcal | |
| 653 | 0 | |a Genetics | |
| 700 | 1 | |a Gallagher, Sean R. | |
| 776 | 0 | 8 | |i Print version: |t GUS protocols. |d San Diego : Academic Press, �1992 |w (DLC) 91028623 |w (OCoLC)24429114 |
| 856 | 4 | 0 | |u https://sciencedirect.uam.elogim.com/science/book/9780122740107 |z Texto completo |