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RNA methodologies : a laboratory guide for isolation and characterization /

This book is a collection of tried and tested laboratory protocols for the isolation and characterization of mammalian RNA. It studies cellular regulation using RNA as a parameter of gene express, offers RNA isolation strategies, and explains proper handling, storage, and manipulation of RNA. * Stud...

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Detalles Bibliográficos
Clasificación:Libro Electrónico
Autor principal: Farrell, Robert E., Jr
Formato: Electrónico eBook
Idioma:Inglés
Publicado: San Diego : Academic Press, �1993.
Temas:
Acceso en línea:Texto completo
Tabla de Contenidos:
  • RNA and the cellular biochemistry revisited
  • Transcription and the organization of eukaryotic gene sequences
  • Messenger RNA
  • Resilient ribonucleases
  • RNA isolation strategies
  • Isolation of polyadenylated RNA
  • Electrophoresis : principles and parameters
  • Electrophoresis of RNA
  • The northern blot
  • Nucleic acid probe technology
  • Practical nucleic acid hybridization
  • Principles of detection
  • Quantitation of specific messenger RNAs by the S1 nuclease assay
  • Analysis of nuclear RNA
  • An RNA paradigm.