Cargando…
Maximizing gene expression /
Maximizing Gene Expression focuses on prokaryotic and eukaryotic gene expression.
| Clasificación: | Libro Electrónico |
|---|---|
| Otros Autores: | , |
| Formato: | Electrónico eBook |
| Idioma: | Inglés |
| Publicado: |
Boston :
Butterworths,
�1986.
|
| Colección: | Biotechnology (Reading, Mass.) ;
9. |
| Temas: | |
| Acceso en línea: | Texto completo |
Tabla de Contenidos:
- Front Cover; Maximizing Gene Expression; Copyright Page; Contributors; Table of Contents; Preface; Chapter 1. E. Coli Promoters; 1.1 Defining Promoters; 1.2 Structure Analysis; 1.3 Steps in Transcription Initiation; 1.4 Structure-Function Correlation; 1.5 Regulation of Transcription Initiation; 1.6 Conclusion; References; Chapter 2. Yeast Promoters; 2.1 Transcription in Yeast; 2.2 Methods for Studying Yeast Promoters; 2.3 Upstream Promoter Elements; 2.4 The TATA Promoter Element; 2.5 Elements that Select Initiation Sites; 2.6 Transcription Regulation; 2.7 Regulatory Proteins
- 2.8 Other Aspects of Regulation2.9 Complex Promoter Organization; 2.10 Molecular Mechanisms: Inferences and Speculations; References; Chapter 3. Protein Coding Genes of Higher Eukaryotes: Promoter Elements and trans-Acting Factors; 3.1 The TATA Box and the Cap Site; 3.2 The Upstream Promoter Elements; 3.3 Enhancer Elements; 3.4 Other trans-Acting Factors; 3.5 Conclusion and Prospects; References; Chapter 4. The Instability of Messenger RNA in Bacteria; 4.1 Some Fundamental Observations and Their Significance; 4.2 Mechanistic Models; 4.3 Search for Specific Enzymes for mRNA Degradation
- 4.4 The Search for Targets4.5 Searching for New Ends; 4.6 Searching for a Model; 4.7 Conclusions; References; Chapter 5. Replication Control of the ColE1-Type Plasmids; 5.1 Incompatibility; 5.2 Replication of ColE1 DNA in Vitro; 5.3 RNA I and Primer Processing; 5.4 RNA I Secondary Structure; 5.5 Mutations in RNA I and Primer That Define Domains of Interaction; 5.6 Analysis of the RNA I-Primer Interaction; 5.7 The Replication Primer; 5.8 Replication-Defective Mutants; 5.9 Temperature-Sensitive Replication Mutants; 5.10 The rop Function; 5.11 Partition and Stability Functions
- Chapter 8. Biased Codon Usage: An Exploration of Its Role in Optimization of Translation8.1 Codon Usage; 8.2 Physiological Aspects of Codon Usage; 8.3 Codon Context and tRNA-tRNA Interaction; 8.4 Evolutionary Aspects of Codon Usage; 8.5 Altering Codon Bias Experimentally; References; Chapter 9. The Selective Degradation of Abnormal Proteins in Bacteria; 9.1 Proteins Rapidly Hydrolyzed in E. Coli; 9.2 Intracellular Aggregates of Abnormal Polypeptides; 9.3 The Energy Requirement and Pathway for Protein Breakdown; 9.4 ATP-Stimulated Proteolysis in Cell-Free Extracts